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The map label for this gene is ompF [H]

Identifier: 218548445

GI number: 218548445

Start: 1113208

End: 1114278

Strand: Reverse

Name: ompF [H]

Synonym: EFER_1073

Alternate gene names: 218548445

Gene position: 1114278-1113208 (Counterclockwise)

Preceding gene: 218548446

Following gene: 218548444

Centisome position: 24.28

GC content: 47.43

Gene sequence:

>1071_bases
ATGATGAAGCGCAATATTCTGGCAGTGGTCGTCCCTGCTCTGTTGGTAGCAGGTACCGCAAACGCTGCGGAAATCTATAA
CAAAGATGGCAACAAACTGGATCTGTACGGTAAAGCTGTCGGTTTGCATTATTTCTCCAAGGGTAACGGTGAAAACAGTT
ACGGTGGCAATGGCGATAAGACCTATGCCCGTCTTGGTTTTAAAGGGGAAACTCAGATTAACTCCGATCTGACCGGCTAC
GGTCAGTGGGAATACAACTTCCAGGGTAATAACTCTGAAGGTGATGATGCTCAGAATGGCAATAAAACGCGTCTGGCATT
TGCTGGTCTGAAATTCGCTGATGCGGGTTCTTTCGATTATGGTCGTAACTACGGCGTGGTTTATGATGCCCTGGGTTATA
CCGATATGCTGCCTGAGTTTGGTGGTGATACCGCTTACAGCGATGACTTCTTCGTAGGTCGTGTTGGCGGCGTTGCAACT
TACCGTAACTCCAACTTCTTTGGTCTGGTTGACGGTCTGAATTTCGCTGTTCAGTACCTGGGTAAAAACGAGCGTGACAC
TGCACGTCGTTCTAACGGCGACGGTGTTGGCGGCTCTATCAGCTATGAATTTGAAGGCTTTGGTATCGTTGGTGCTTATG
GTGCTGCAGACCGTACCAACCTGCAGGAAGACTCTACCCTTGGCAAAGGCAAAAAAGCTGAACAGTGGGCTACTGGCCTG
AAGTATGATGCTAACAACATCTATCTGGCAGCTAACTACGGTGAAACCCGTAATGCTACGCCGATCACTGGTGGCTTTGC
CAACAAAACACAAGATATCCTGTTAGTTGCGCAATACCAGTTCGACTTCGGTCTGCGTCCGTCCATCGCCTATACCAAAT
CTAAAGCGAAAGATGTAGAAGGTATCGGTGATGTTGATCTGGTGAACTACATTGAAGTGGGCGCAACCTACTACTTCAAC
AAAAACATGTCTACCTATGTTGACTATGCGATTAACCAAATCGATTCTGACAACAAGCTGGGCGTAGGTTCTGACGATAC
AGTTGCTGTTGGTATCGTTTACCAGTTCTAA

Upstream 100 bases:

>100_bases
AGACACCAAACTTTCATCAATAGTTCCGTGAATATTTATTTACAGAATTTATTGGCGGCAGTGGCAGGTGTTATAAAAAA
AACCATGAGGGTAATAAATA

Downstream 100 bases:

>100_bases
TAGCATACCCCTTTGTTAAATGCCTAAAAAACAGGACGTTGGTCCTGTTTTTTTATACCTGATAGAGAAATCTTGCATCT
GAAAAAAACAGTCAGAGTTT

Product: outer membrane protein F

Products: NA

Alternate protein names: Outer membrane protein 1A; Outer membrane protein B; Outer membrane protein IA; Porin OmpF [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MMKRNILAVVVPALLVAGTANAAEIYNKDGNKLDLYGKAVGLHYFSKGNGENSYGGNGDKTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGDDAQNGNKTRLAFAGLKFADAGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEFEGFGIVGAYGAADRTNLQEDSTLGKGKKAEQWATGL
KYDANNIYLAANYGETRNATPITGGFANKTQDILLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYIEVGATYYFN
KNMSTYVDYAINQIDSDNKLGVGSDDTVAVGIVYQF

Sequences:

>Translated_356_residues
MMKRNILAVVVPALLVAGTANAAEIYNKDGNKLDLYGKAVGLHYFSKGNGENSYGGNGDKTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGDDAQNGNKTRLAFAGLKFADAGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEFEGFGIVGAYGAADRTNLQEDSTLGKGKKAEQWATGL
KYDANNIYLAANYGETRNATPITGGFANKTQDILLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYIEVGATYYFN
KNMSTYVDYAINQIDSDNKLGVGSDDTVAVGIVYQF
>Mature_356_residues
MMKRNILAVVVPALLVAGTANAAEIYNKDGNKLDLYGKAVGLHYFSKGNGENSYGGNGDKTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGDDAQNGNKTRLAFAGLKFADAGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEFEGFGIVGAYGAADRTNLQEDSTLGKGKKAEQWATGL
KYDANNIYLAANYGETRNATPITGGFANKTQDILLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYIEVGATYYFN
KNMSTYVDYAINQIDSDNKLGVGSDDTVAVGIVYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1787160, Length=362, Percent_Identity=93.9226519337017, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1786436, Length=358, Percent_Identity=62.2905027932961, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1788544, Length=376, Percent_Identity=59.8404255319149, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1787641, Length=363, Percent_Identity=58.1267217630854, Blast_Score=383, Evalue=1e-107,

Paralogues:

None

Copy number: 1980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 17600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12744 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 38551; Mature: 38551

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRNILAVVVPALLVAGTANAAEIYNKDGNKLDLYGKAVGLHYFSKGNGENSYGGNGDK
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCC
TYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGDDAQNGNKTRLAFAGLKFADAGSFDY
EEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCEEECCCCCCC
GRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYL
CCCCCEEEECCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCEEEEEHHHHHHHHHH
GKNERDTARRSNGDGVGGSISYEFEGFGIVGAYGAADRTNLQEDSTLGKGKKAEQWATGL
CCCCHHHHHCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCC
KYDANNIYLAANYGETRNATPITGGFANKTQDILLVAQYQFDFGLRPSIAYTKSKAKDVE
EECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCC
GIGDVDLVNYIEVGATYYFNKNMSTYVDYAINQIDSDNKLGVGSDDTVAVGIVYQF
CCCCCHHHHHEEECEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MMKRNILAVVVPALLVAGTANAAEIYNKDGNKLDLYGKAVGLHYFSKGNGENSYGGNGDK
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCC
TYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGDDAQNGNKTRLAFAGLKFADAGSFDY
EEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCEEECCCCCCC
GRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYL
CCCCCEEEECCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCEEEEEHHHHHHHHHH
GKNERDTARRSNGDGVGGSISYEFEGFGIVGAYGAADRTNLQEDSTLGKGKKAEQWATGL
CCCCHHHHHCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCC
KYDANNIYLAANYGETRNATPITGGFANKTQDILLVAQYQFDFGLRPSIAYTKSKAKDVE
EECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCC
GIGDVDLVNYIEVGATYYFNKNMSTYVDYAINQIDSDNKLGVGSDDTVAVGIVYQF
CCCCCHHHHHEEECEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6294623; 8905232; 9278503; 7037455; 7049161; 2997131; 9298646; 9629924; 1380671; 7524100; 9843370 [H]