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The map label for this gene is hemF [H]

Identifier: 218548124

GI number: 218548124

Start: 754946

End: 755845

Strand: Reverse

Name: hemF [H]

Synonym: EFER_0736

Alternate gene names: 218548124

Gene position: 755845-754946 (Counterclockwise)

Preceding gene: 218548125

Following gene: 218548123

Centisome position: 16.47

GC content: 54.67

Gene sequence:

>900_bases
ATGAAACCCGACGCACACCAGGTTAAACAGTTCCTGCTCAACCTTCAGGATACAATTTGTCAGCAGTTGACCGCTGTCGA
TGGCGCAGAGTTTGTCGAAGATAGCTGGCAGCGCGAAGCTGGCGGCGGTGGGCGTAGTCGGGTGTTGCGTAATGGTGGTG
TTTTCGAACAGGCGGGCGTCAACTTTTCACATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACTGCTCATCGCCCGGAA
CTTGCCGGACGCAGTTTCGAGGCGATGGGCGTTTCACTGGTAGTGCATCCGCATAATCCGTATGTTCCCACCAGCCACGC
GAATGTGCGCTTTTTTATTGCCGAAAAACCAGGTGCCGAGCCCGTCTGGTGGTTTGGCGGCGGCTTCGATTTAACCCCTT
TCTATGGTTTTGAAGAAGACGCCATTCACTGGCACCGCACCGCCCGTGACCTGTGCCTGCCATTTGGCGAAGACGTTTAT
CCGCGTTACAAAAAGTGGTGCGACGAATACTTCTACCTCAAACATCGTAACGAACAGCGCGGTATTGGCGGGCTTTTCTT
TGATGACCTGAACACGCCAGACTTTGACCACTGTTTTGCCTTTATGCAGGCGGTAGGTAAAGGCTACACCGACGCTTATT
TACCGATTGTTGAACGACGCAAAGCGATGGAATACGGCGAGCACGAGCGCAATTTCCAGCTCTACCGTCGCGGTCGCTAT
GTCGAGTTCAACCTGGTCTGGGATCGCGGCACGCTGTTTGGCCTGCAAACTGGCGGGCGCACCGAGTCTATCCTGATGTC
AATGCCGCCGCTGGTACGCTGGGAGTATGATTATCAGCCAGAAGCAGGCAGCCCGGAAGCGGCGTTAAGTGAGTTTATTA
AGGTCAGGGATTGGGTTTAA

Upstream 100 bases:

>100_bases
GGCATTTCGCCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCATTGGTTCGACAACCAGAAAGCAC
ATTCGAAAAAGCGATAAGTT

Downstream 100 bases:

>100_bases
TATCATGAAAATAAAAGAAAAATAACCCTATGTTTGTGGCTTGAAATTAATTCTGTTTAATTTGTGTGCCATAGTGACAC
CCTAATATCTAATAATAATC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMEYGEHERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPEAGSPEAALSEFIKVRDWV

Sequences:

>Translated_299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMEYGEHERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPEAGSPEAALSEFIKVRDWV
>Mature_299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMEYGEHERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPEAGSPEAALSEFIKVRDWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=303, Percent_Identity=46.8646864686469, Blast_Score=282, Evalue=3e-76,
Organism=Escherichia coli, GI1788777, Length=299, Percent_Identity=97.9933110367893, Blast_Score=612, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6320249, Length=317, Percent_Identity=43.8485804416404, Blast_Score=258, Evalue=6e-70,
Organism=Drosophila melanogaster, GI17864388, Length=304, Percent_Identity=48.0263157894737, Blast_Score=288, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 34314; Mature: 34314

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCHHCCC
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAE
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMEYGEHERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPEAGSPEAALSEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCHHCCC
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAE
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMEYGEHERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPEAGSPEAALSEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA