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The map label for this gene is amiA [H]

Identifier: 218548125

GI number: 218548125

Start: 755849

End: 756718

Strand: Reverse

Name: amiA [H]

Synonym: EFER_0737

Alternate gene names: 218548125

Gene position: 756718-755849 (Counterclockwise)

Preceding gene: 218548130

Following gene: 218548124

Centisome position: 16.49

GC content: 50.57

Gene sequence:

>870_bases
ATGAGCACTTTTAAACCACTAAAAACACTCACTTCGCGCCGCCAGGTGCTGAAAGCAGGGCTTGCAGCTCTGACGCTGTC
AGGGATGTCGCAAGCCATCGCCAAAGAAGAACCTTTAAAAACCAGTAACGGACACAGTAAGCCGAAAGCCAAAAAAGCCG
GGAGCAAACGGGTAGTCGTTCTCGATCCAGGCCACGGCGGAATTGATACCGGAGCGATTGGTCGCAACGGCTCGAAAGAA
AAACACGTGGTGCTGGCGATTGCCAAAAATGTTCGTTCCATTTTGCGCAATCACGGCATTGATGCGCGTTTAACGCGTTC
TGGCGATACGTTTATCCCGCTGTACGATCGCGTTGAAATTGCCCATAAGCATCGCGCAGATCTGTTTATGTCAATTCACG
CCGACGGTTTTACTAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCG
AAGTACCTGTCTGAACGCGAAAACCGCGCTGATGAAGTTGCCGGAAAAAAAGCGACTGACAAGGATCACCTATTGCAGCA
AGTGCTGTTTGATCTGGTGCAAACCGATACCATTAAAAACAGCCTGACGCTTGGCTCGCATATTCTGAAAAAGATTAAGC
CGGTGCATAAGCTGCACAGTCGCAATACCGAACAAGCCGCGTTTGTGGTGTTGAAATCGCCGTCTGTCCCTTCTGTGCTG
GTGGAAACCTCGTTTATCACCAACCCGGAAGAAGAGCGGCTGTTAGGCACGGCGGCATTTCGCCAGAAAATCGCCACAGC
GATTGCTGAAGGCGTGATCAGTTATTTCCATTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAA

Upstream 100 bases:

>100_bases
ATTGAAATGAAAAGTAAAACAATTCTCAACATCAAGCGGTCGTAACGGAATACGCGATACGATATAACATCTGGAACTTT
ATTATTACAACTCAGGCCGT

Downstream 100 bases:

>100_bases
GTTATGAAACCCGACGCACACCAGGTTAAACAGTTCCTGCTCAACCTTCAGGATACAATTTGTCAGCAGTTGACCGCTGT
CGATGGCGCAGAGTTTGTCG

Product: N-acetylmuramoyl-l-alanine amidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNGHSKPKAKKAGSKRVVVLDPGHGGIDTGAIGRNGSKE
KHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHRADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR

Sequences:

>Translated_289_residues
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNGHSKPKAKKAGSKRVVVLDPGHGGIDTGAIGRNGSKE
KHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHRADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
>Mature_288_residues
STFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNGHSKPKAKKAGSKRVVVLDPGHGGIDTGAIGRNGSKEK
HVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHRADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAK
YLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVLV
ETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=289, Percent_Identity=98.2698961937716, Blast_Score=575, Evalue=1e-166,
Organism=Escherichia coli, GI87082163, Length=235, Percent_Identity=48.5106382978723, Blast_Score=227, Evalue=5e-61,
Organism=Escherichia coli, GI1790611, Length=244, Percent_Identity=38.1147540983607, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31524; Mature: 31392

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNGHSKPKAKKAGSKRVVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEE
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHH
AHKHRADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
STFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNGHSKPKAKKAGSKRVVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEE
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHH
AHKHRADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]