Click here to switch to the map view.

The map label for this gene is yfhD [H]

Identifier: 218547915

GI number: 218547915

Start: 514206

End: 515624

Strand: Reverse

Name: yfhD [H]

Synonym: EFER_0517

Alternate gene names: 218547915

Gene position: 515624-514206 (Counterclockwise)

Preceding gene: 218547923

Following gene: 218547912

Centisome position: 11.24

GC content: 50.39

Gene sequence:

>1419_bases
GTGAGCACTATTCATACCCCACTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACA
GTTTGCCGAGTACCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTCGATAACG
GCAACGCCGATCTGCTGGCTGCAGGACTGGTTTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTACTAT
TCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCGCGTACGCTGGGCAATCTGACAGCGGAGCAACTTAC
CATCGCACCAGGCCATGTAGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAG
ACGACAAAAAAGGCTCAGCAGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCC
ATCAGCCTGTTTCAGCGCGTTCACCCGGAACTCGCTGTAGCGCTTGATATCACCGACGAACAGCCGGTGACCTGGTTTAG
CCGATTAGATGGCGATAATACCCTTTCTGCCGCTCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGCACGCTGGCAC
GCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACGTTTTTACGCGCCGTCGATACC
GTACTGCCTCAGTTGAAACCGCTGTTTGAGAAGTACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCA
GGAATCGCACTGGGATGCGCAGGCCACTTCACCGACGGGCGTGCGCGGCATGATGATGTTAACCAAAAATACCGCGCAAA
GCCTCGGCATTACGGATCGTACCGATGCCGAACAGAGCATCAGCGGCGGTGTGCGTTATTTGCAGGATATGATGAGTAAA
GTGCCGGAAAGTGTGCCGGAGAACGAACGGATCTGGTTTGCCCTCGCCGCGTACAATATGGGCTATGCGCATATGCTGGA
TGCCCGCGCCCTGACGGCAAAAACTAAAGGTAATCCTGACAGTTGGGCTGACGTGAAACAGCGCCTGCCTTTACTTAGCC
AGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCACGTGGGCATGAAGCCTACGCTTATGTCGAAAATATTCGTAAG
TATCAGATTAGCCTGGTGGGGTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATACAACTGGCGCAGGATTA
TCCGGCAGTATCGCCAACGGAGCTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATT
TGACCCACTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAA

Upstream 100 bases:

>100_bases
TCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTC
AAGCGCGGGGAGAGTTGCGT

Downstream 100 bases:

>100_bases
TCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGTCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCA
CTCATCCGCCAGTATTCCTT

Product: transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTIHTPLTYNEINGKPFGLDYELAKQFAEYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYY
SVSQQLVYKVGQYRPRTLGNLTAEQLTIAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVA
ISLFQRVHPELAVALDITDEQPVTWFSRLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDT
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSK
VPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRK
YQISLVGYLQEKEKQATEAAIQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Sequences:

>Translated_472_residues
MSTIHTPLTYNEINGKPFGLDYELAKQFAEYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYY
SVSQQLVYKVGQYRPRTLGNLTAEQLTIAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVA
ISLFQRVHPELAVALDITDEQPVTWFSRLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDT
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSK
VPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRK
YQISLVGYLQEKEKQATEAAIQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
>Mature_471_residues
STIHTPLTYNEINGKPFGLDYELAKQFAEYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYS
VSQQLVYKVGQYRPRTLGNLTAEQLTIAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAI
SLFQRVHPELAVALDITDEQPVTWFSRLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDTV
LPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKV
PESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKY
QISLVGYLQEKEKQATEAAIQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=472, Percent_Identity=98.728813559322, Blast_Score=963, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 53257; Mature: 53126

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIHTPLTYNEINGKPFGLDYELAKQFAEYLGVKLKVTVRQNISQLFDDLDNGNADLLA
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEE
AGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTIAPGHVVVNDLQT
EHHEECHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHH
LKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDE
HHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCC
QPVTWFSRLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDT
CCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHCCCCCC
TDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHEECCCCCCC
SWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAA
HHHHHHHHCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
HHHHHHCCCCCHHHCCCHHCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC
>Mature Secondary Structure 
STIHTPLTYNEINGKPFGLDYELAKQFAEYLGVKLKVTVRQNISQLFDDLDNGNADLLA
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEE
AGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTIAPGHVVVNDLQT
EHHEECHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHH
LKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDE
HHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCC
QPVTWFSRLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDT
CCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHCCCCCC
TDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHEECCCCCCC
SWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAA
HHHHHHHHCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
HHHHHHCCCCCHHHCCCHHCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA