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The map label for this gene is phoA [H]

Identifier: 218232770

GI number: 218232770

Start: 2879324

End: 2880997

Strand: Reverse

Name: phoA [H]

Synonym: BCB4264_A3003

Alternate gene names: 218232770

Gene position: 2880997-2879324 (Counterclockwise)

Preceding gene: 218235230

Following gene: 218234704

Centisome position: 53.16

GC content: 38.05

Gene sequence:

>1674_bases
ATGTTGAATTATGGTGTGAAGATGAAAAAGTTGCTATTAGTGGCAGCTATAATAGTAAGCGTATTAAGTACGGAAAGTAT
GATGAATTATCATGCAGCCAAAGCTAAGGTGAAAAAAGTAGAACATCAGCCTAAAAATATAATTATGATGGTTATGGACG
GAACGAGTTCAACGGCGACAACATTGGCTCGCTGGTATAAAGGGGCACCGCTTACGTTAGATCAAATTGTAACAGGAGGA
GTTCGTACGTTTTCGGCGGAATCAGCTATTACAGACTCAGCGCCAGCAGCAACAGCATTAGCAACTGGAAATAAATCAAA
TTCAGGATATGTAGGTGTATTACCTTCTATTGTAAGTTCGCCTAGCTTAGAGCCAATAAAAGAAGAGGACAAGTTACGGC
CGGTCGCAAACGTATTAGAAGGTGCAAAACGTACTGGTCGTGCAACTGGAATTGTTGCTACATCTGAAATTCAGCATGCT
ACTCCGGCGGGATTCTCGGCTCACCATGTCAATCGCAAGCAATTTGATGTAATTGCGAAGCAGCAAGTATATCAAAATAT
AGATGTCGTATTAGGCGGGGGAAAAGCGGCGCTGTTACCTATGCAAAGTAATGGAATCCGAAAAGATGGTGAAAATTTAG
TAAAGGTGATTCAAAACAAAGGATACGATTTTGTTGAAACGAAAGATGCGTTATTGAACTCCAAATCTAATAAAATTTGG
GGCTCTTTCTCACATCATGCTCTTGCGTTTGATACGGATCGTATAGCAACAAACCCAGAACAACCAACACTTTCTCAAAT
GACAGAAAAAGCAATTCAAACATTATCAAAAGATAAAGATGGCTTCTTTTTATTCATAGAAGGTAGTAAGCCTGATTGGG
CAGCCCATGCGAATGATCCAATTGGGATGATTAGTGATATATTGGCATTTGATGAAGCGGTAGCAAAGGCGTTACAATTT
GCGAAGAAAGATGGGGATACGATGCTCATCGCTTTAACTGATCATGGTAACAGCGGCATCTCTATAGGGAATAGGAATAC
GACAAAAGGATACAATACTACACCGGTTTCTGCATACATTGATCCATTGAAAAAGGCGAAAATGACACTTGAAGGAACTA
TAAATAAACTGAAATCTGATTTATCAAATGTAGAAGATGTAGCTAAACTATACGGTTTGGATAATCTAACTCATGATGAA
AAAGAAAAATTAAAAGCGGCAAAAAAGAAAATGGATGTAGGACGAATTCTTACTACATTATTGGCTAATCGTGCAAATAT
CGGCTTTACGACAGGAGGACATACAGGTGAGGATGTATTCTTACATTCTTATGGACCTCAAAAACCAGAAGGCTTAATCC
AAAATACAGACATTGCGAAGACGATGGCGAAAGCGATGGGCTTTAACTTAGAAGAAGTAACGAATAAAATATTTTTAGAG
AGTGAACAAGCCTTTAAGAAGATCGGTGCAACTGTAACAGCCAACAAAACCGAAGGAGCAAACCCGTTACTGGTAGTAAA
ACATAATAAAGTAGAAGCACAATTATTTGGTAACAAAAATATAATTCGTATCAATGGTAAAGGGTATGAATTAAGAAGTA
TTATTGTAGAGAGTAATGGAAAGTGCTATGTACCAGAAGAAGCAATTCAACTATTTGTAAAGCATTCTCGTTAA

Upstream 100 bases:

>100_bases
CTTTATGAGAATTGGGAACTAATAAAACATATGTAGGTTTTGGTATGGAAAAGGAAATAGTGTGTATTTAAATGAATGCA
AAGTTTATGGAGGAACAATC

Downstream 100 bases:

>100_bases
AAAATTGCCAACCAGTTAAGCTAACTGGTGGCAATTTTTTGTGTTTATTCTAAATGTAAGTGTTCGAATTGTTTTGTAAA
GTTTTCCATATGAGACTGGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MLNYGVKMKKLLLVAAIIVSVLSTESMMNYHAAKAKVKKVEHQPKNIIMMVMDGTSSTATTLARWYKGAPLTLDQIVTGG
VRTFSAESAITDSAPAATALATGNKSNSGYVGVLPSIVSSPSLEPIKEEDKLRPVANVLEGAKRTGRATGIVATSEIQHA
TPAGFSAHHVNRKQFDVIAKQQVYQNIDVVLGGGKAALLPMQSNGIRKDGENLVKVIQNKGYDFVETKDALLNSKSNKIW
GSFSHHALAFDTDRIATNPEQPTLSQMTEKAIQTLSKDKDGFFLFIEGSKPDWAAHANDPIGMISDILAFDEAVAKALQF
AKKDGDTMLIALTDHGNSGISIGNRNTTKGYNTTPVSAYIDPLKKAKMTLEGTINKLKSDLSNVEDVAKLYGLDNLTHDE
KEKLKAAKKKMDVGRILTTLLANRANIGFTTGGHTGEDVFLHSYGPQKPEGLIQNTDIAKTMAKAMGFNLEEVTNKIFLE
SEQAFKKIGATVTANKTEGANPLLVVKHNKVEAQLFGNKNIIRINGKGYELRSIIVESNGKCYVPEEAIQLFVKHSR

Sequences:

>Translated_557_residues
MLNYGVKMKKLLLVAAIIVSVLSTESMMNYHAAKAKVKKVEHQPKNIIMMVMDGTSSTATTLARWYKGAPLTLDQIVTGG
VRTFSAESAITDSAPAATALATGNKSNSGYVGVLPSIVSSPSLEPIKEEDKLRPVANVLEGAKRTGRATGIVATSEIQHA
TPAGFSAHHVNRKQFDVIAKQQVYQNIDVVLGGGKAALLPMQSNGIRKDGENLVKVIQNKGYDFVETKDALLNSKSNKIW
GSFSHHALAFDTDRIATNPEQPTLSQMTEKAIQTLSKDKDGFFLFIEGSKPDWAAHANDPIGMISDILAFDEAVAKALQF
AKKDGDTMLIALTDHGNSGISIGNRNTTKGYNTTPVSAYIDPLKKAKMTLEGTINKLKSDLSNVEDVAKLYGLDNLTHDE
KEKLKAAKKKMDVGRILTTLLANRANIGFTTGGHTGEDVFLHSYGPQKPEGLIQNTDIAKTMAKAMGFNLEEVTNKIFLE
SEQAFKKIGATVTANKTEGANPLLVVKHNKVEAQLFGNKNIIRINGKGYELRSIIVESNGKCYVPEEAIQLFVKHSR
>Mature_557_residues
MLNYGVKMKKLLLVAAIIVSVLSTESMMNYHAAKAKVKKVEHQPKNIIMMVMDGTSSTATTLARWYKGAPLTLDQIVTGG
VRTFSAESAITDSAPAATALATGNKSNSGYVGVLPSIVSSPSLEPIKEEDKLRPVANVLEGAKRTGRATGIVATSEIQHA
TPAGFSAHHVNRKQFDVIAKQQVYQNIDVVLGGGKAALLPMQSNGIRKDGENLVKVIQNKGYDFVETKDALLNSKSNKIW
GSFSHHALAFDTDRIATNPEQPTLSQMTEKAIQTLSKDKDGFFLFIEGSKPDWAAHANDPIGMISDILAFDEAVAKALQF
AKKDGDTMLIALTDHGNSGISIGNRNTTKGYNTTPVSAYIDPLKKAKMTLEGTINKLKSDLSNVEDVAKLYGLDNLTHDE
KEKLKAAKKKMDVGRILTTLLANRANIGFTTGGHTGEDVFLHSYGPQKPEGLIQNTDIAKTMAKAMGFNLEEVTNKIFLE
SEQAFKKIGATVTANKTEGANPLLVVKHNKVEAQLFGNKNIIRINGKGYELRSIIVESNGKCYVPEEAIQLFVKHSR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=357, Percent_Identity=28.8515406162465, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI94721246, Length=347, Percent_Identity=28.8184438040346, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI157266296, Length=345, Percent_Identity=29.2753623188406, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI116734717, Length=336, Percent_Identity=27.6785714285714, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI294660770, Length=332, Percent_Identity=27.1084337349398, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI294660772, Length=288, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI48994877, Length=348, Percent_Identity=33.0459770114943, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=530, Percent_Identity=30.377358490566, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24657827, Length=490, Percent_Identity=28.3673469387755, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24657835, Length=467, Percent_Identity=28.6937901498929, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21355981, Length=325, Percent_Identity=29.2307692307692, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI85725200, Length=330, Percent_Identity=28.7878787878788, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI85815833, Length=330, Percent_Identity=28.7878787878788, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921912, Length=345, Percent_Identity=28.1159420289855, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21355151, Length=372, Percent_Identity=27.4193548387097, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24657842, Length=467, Percent_Identity=28.2655246252677, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21358067, Length=389, Percent_Identity=28.0205655526992, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859923, Length=427, Percent_Identity=27.6346604215457, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355149, Length=339, Percent_Identity=28.023598820059, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585213, Length=340, Percent_Identity=27.9411764705882, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24651554, Length=360, Percent_Identity=26.1111111111111, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24651556, Length=303, Percent_Identity=26.0726072607261, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 60381; Mature: 60381

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYGVKMKKLLLVAAIIVSVLSTESMMNYHAAKAKVKKVEHQPKNIIMMVMDGTSSTAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
TLARWYKGAPLTLDQIVTGGVRTFSAESAITDSAPAATALATGNKSNSGYVGVLPSIVSS
HHHHHHCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCCCCEEEECHHHHCC
PSLEPIKEEDKLRPVANVLEGAKRTGRATGIVATSEIQHATPAGFSAHHVNRKQFDVIAK
CCCCCCCHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHCCCHHHHHHHHH
QQVYQNIDVVLGGGKAALLPMQSNGIRKDGENLVKVIQNKGYDFVETKDALLNSKSNKIW
HHHHCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
GSFSHHALAFDTDRIATNPEQPTLSQMTEKAIQTLSKDKDGFFLFIEGSKPDWAAHANDP
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
IGMISDILAFDEAVAKALQFAKKDGDTMLIALTDHGNSGISIGNRNTTKGYNTTPVSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCHHHHH
DPLKKAKMTLEGTINKLKSDLSNVEDVAKLYGLDNLTHDEKEKLKAAKKKMDVGRILTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LANRANIGFTTGGHTGEDVFLHSYGPQKPEGLIQNTDIAKTMAKAMGFNLEEVTNKIFLE
HHCCCCCCEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCC
SEQAFKKIGATVTANKTEGANPLLVVKHNKVEAQLFGNKNIIRINGKGYELRSIIVESNG
CHHHHHHHCCEEEECCCCCCCCEEEEECCCEEEEEECCCCEEEECCCCEEEEEEEEECCC
KCYVPEEAIQLFVKHSR
CEECCHHHHHHHHHCCC
>Mature Secondary Structure
MLNYGVKMKKLLLVAAIIVSVLSTESMMNYHAAKAKVKKVEHQPKNIIMMVMDGTSSTAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
TLARWYKGAPLTLDQIVTGGVRTFSAESAITDSAPAATALATGNKSNSGYVGVLPSIVSS
HHHHHHCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCCCCEEEECHHHHCC
PSLEPIKEEDKLRPVANVLEGAKRTGRATGIVATSEIQHATPAGFSAHHVNRKQFDVIAK
CCCCCCCHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHCCCHHHHHHHHH
QQVYQNIDVVLGGGKAALLPMQSNGIRKDGENLVKVIQNKGYDFVETKDALLNSKSNKIW
HHHHCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
GSFSHHALAFDTDRIATNPEQPTLSQMTEKAIQTLSKDKDGFFLFIEGSKPDWAAHANDP
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
IGMISDILAFDEAVAKALQFAKKDGDTMLIALTDHGNSGISIGNRNTTKGYNTTPVSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCHHHHH
DPLKKAKMTLEGTINKLKSDLSNVEDVAKLYGLDNLTHDEKEKLKAAKKKMDVGRILTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LANRANIGFTTGGHTGEDVFLHSYGPQKPEGLIQNTDIAKTMAKAMGFNLEEVTNKIFLE
HHCCCCCCEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCC
SEQAFKKIGATVTANKTEGANPLLVVKHNKVEAQLFGNKNIIRINGKGYELRSIIVESNG
CHHHHHHHCCEEEECCCCCCCCEEEEECCCEEEEEECCCCEEEECCCCEEEEEEEEECCC
KCYVPEEAIQLFVKHSR
CEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]