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The map label for this gene is paaF [C]

Identifier: 209551989

GI number: 209551989

Start: 62808

End: 63614

Strand: Reverse

Name: paaF [C]

Synonym: Rleg2_6129

Alternate gene names: 209551989

Gene position: 63614-62808 (Counterclockwise)

Preceding gene: 209551990

Following gene: 209551988

Centisome position: 20.6

GC content: 60.59

Gene sequence:

>807_bases
ATGACCGAACCTGCGATCCTGTTCGACGTTGACCGGACGGCAAAACGCCTCAACGAGATATCCGAGCGAAGCGGCAAGAT
CGTTCGGATCGAATTATCCCGACCGGAGCGTGCCAACGCGCTGAATTCCGAGACTTTGGAACTCATTCTCGATGCTATCG
AGAAGGCGGAGGTTGAGCCAGGCGTGGAGGCCATCGTCCTGCATGGAGCAGGACAGCACTTCTGCGCGGGCGCAGACCTA
AGGGAGCTTCATTCCGGCGGTCCCGCAGGCATCAGGAGGCTGCTCGATCTGTTCCGGCGTGTCACGATCAGGATTGAACG
GTCCCATCTCGTGGTCATCGCCGCAGTTCACGGCGCCGCCCGGGCCGGCGGGCTGGAGATCTCGCTCGCATGTGACGTCG
TGCTCGCGGGAAAGTCCGCGACGTTCGGGGATGCGCATCTCTCGAAGGGGCTGCTGCCCGGCGGGGGATCGACTGTTCGC
CTTCCCCGCGCGATCGGCTGGCAGAGAAGCAAATGGATGATCCTCAGCGCCGAGGCGATCGATGCCGAGACTGCCAAACA
GTGGGGACTCGTTTTCGACGTCGTCGATGACGTAGATCTTCTCCAGGCCGCTAAGGATCAGGCTTTGCGAATGACGCGCG
GAGACACCGAAGCAATGGGGCGAGTGAAGAAACTGATTTCCATGGTCGGCGAGCAATTCTTCAGCGACGGCCTGGAAGCC
GAAATTGCGACGCTGGAGACGCATTCGCAGTCGGAGTCCTTTCAAAACGGCGTCGGCAGCTTTTTGACGCGTAACAAGCG
CGGCTAA

Upstream 100 bases:

>100_bases
GGTAAGGCATATTGTCGAGAACCCATATCTGAACGGGTCTCTCATTCGGCTGGATGGCGGCGCCCGCCTTCCTGCCTGAC
GCCCGGAAAAGGAAAAACAT

Downstream 100 bases:

>100_bases
CCAGAACCGTCTAAATTGCAGGAGTATGAATATGCAAGAAAACACAAATCCTGTTGTCATTGTTTCGGCGGCTCGCACAC
CGATCGGCTCGTTTCAGGGT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTEPAILFDVDRTAKRLNEISERSGKIVRIELSRPERANALNSETLELILDAIEKAEVEPGVEAIVLHGAGQHFCAGADL
RELHSGGPAGIRRLLDLFRRVTIRIERSHLVVIAAVHGAARAGGLEISLACDVVLAGKSATFGDAHLSKGLLPGGGSTVR
LPRAIGWQRSKWMILSAEAIDAETAKQWGLVFDVVDDVDLLQAAKDQALRMTRGDTEAMGRVKKLISMVGEQFFSDGLEA
EIATLETHSQSESFQNGVGSFLTRNKRG

Sequences:

>Translated_268_residues
MTEPAILFDVDRTAKRLNEISERSGKIVRIELSRPERANALNSETLELILDAIEKAEVEPGVEAIVLHGAGQHFCAGADL
RELHSGGPAGIRRLLDLFRRVTIRIERSHLVVIAAVHGAARAGGLEISLACDVVLAGKSATFGDAHLSKGLLPGGGSTVR
LPRAIGWQRSKWMILSAEAIDAETAKQWGLVFDVVDDVDLLQAAKDQALRMTRGDTEAMGRVKKLISMVGEQFFSDGLEA
EIATLETHSQSESFQNGVGSFLTRNKRG
>Mature_267_residues
TEPAILFDVDRTAKRLNEISERSGKIVRIELSRPERANALNSETLELILDAIEKAEVEPGVEAIVLHGAGQHFCAGADLR
ELHSGGPAGIRRLLDLFRRVTIRIERSHLVVIAAVHGAARAGGLEISLACDVVLAGKSATFGDAHLSKGLLPGGGSTVRL
PRAIGWQRSKWMILSAEAIDAETAKQWGLVFDVVDDVDLLQAAKDQALRMTRGDTEAMGRVKKLISMVGEQFFSDGLEAE
IATLETHSQSESFQNGVGSFLTRNKRG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=29.4117647058824, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI4502327, Length=199, Percent_Identity=32.6633165829146, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI70995211, Length=187, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI68989263, Length=221, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=25.462962962963, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=25.462962962963, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI20127408, Length=136, Percent_Identity=30.8823529411765, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI157694520, Length=201, Percent_Identity=26.865671641791, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=32.6530612244898, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787660, Length=245, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI221142681, Length=217, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=33.5078534031414, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=25.2, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788597, Length=181, Percent_Identity=29.2817679558011, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=28.8659793814433, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=31.9502074688797, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=171, Percent_Identity=30.4093567251462, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=236, Percent_Identity=28.8135593220339, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=25, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=206, Percent_Identity=30.0970873786408, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=203, Percent_Identity=31.5270935960591, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=238, Percent_Identity=23.9495798319328, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653139, Length=183, Percent_Identity=35.5191256830601, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20129971, Length=232, Percent_Identity=28.8793103448276, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653477, Length=232, Percent_Identity=28.8793103448276, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24650670, Length=176, Percent_Identity=31.8181818181818, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19920382, Length=262, Percent_Identity=24.4274809160305, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=29.1208791208791, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654903, Length=249, Percent_Identity=22.0883534136546, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28985; Mature: 28853

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPAILFDVDRTAKRLNEISERSGKIVRIELSRPERANALNSETLELILDAIEKAEVEP
CCCCCEEEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
GVEAIVLHGAGQHFCAGADLRELHSGGPAGIRRLLDLFRRVTIRIERSHLVVIAAVHGAA
CCEEEEEECCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHEEEECCEEEEEEEECCCC
RAGGLEISLACDVVLAGKSATFGDAHLSKGLLPGGGSTVRLPRAIGWQRSKWMILSAEAI
CCCCEEEEEEEEEEEECCCCCCCHHHHHCCCCCCCCCEEECCHHHCCCCCCEEEEEEHHH
DAETAKQWGLVFDVVDDVDLLQAAKDQALRMTRGDTEAMGRVKKLISMVGEQFFSDGLEA
CHHHHHHHCCCEEHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
EIATLETHSQSESFQNGVGSFLTRNKRG
EEEEECCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TEPAILFDVDRTAKRLNEISERSGKIVRIELSRPERANALNSETLELILDAIEKAEVEP
CCCCEEEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
GVEAIVLHGAGQHFCAGADLRELHSGGPAGIRRLLDLFRRVTIRIERSHLVVIAAVHGAA
CCEEEEEECCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHEEEECCEEEEEEEECCCC
RAGGLEISLACDVVLAGKSATFGDAHLSKGLLPGGGSTVRLPRAIGWQRSKWMILSAEAI
CCCCEEEEEEEEEEEECCCCCCCHHHHHCCCCCCCCCEEECCHHHCCCCCCEEEEEEHHH
DAETAKQWGLVFDVVDDVDLLQAAKDQALRMTRGDTEAMGRVKKLISMVGEQFFSDGLEA
CHHHHHHHCCCEEHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
EIATLETHSQSESFQNGVGSFLTRNKRG
EEEEECCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA