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The map label for this gene is proP [H]

Identifier: 209551979

GI number: 209551979

Start: 44137

End: 45795

Strand: Reverse

Name: proP [H]

Synonym: Rleg2_6117

Alternate gene names: 209551979

Gene position: 45795-44137 (Counterclockwise)

Preceding gene: 209551980

Following gene: 209551978

Centisome position: 14.83

GC content: 50.39

Gene sequence:

>1659_bases
ATGGTACAAGTAGACATACTGCCAAAAACAGAGGGGATCTCCTCAAACGAGCGACGCGTCATTGTTGCTGCATCACTCGG
AACAGTTTTTGAATTCTACGACTTTTTTCTCATTGGATTGTTAGCTAATGAAATTTCGAAAGCATTTTTTTCCGGCGTAA
ACCCAACAGCTGGTTTCATCTTTACGCTTCTCGGCTTTGCGGCAGGCTTTTTGTTAAGGCCGTTCGGGGCGATCGTGTTT
GGTCGCCTTGGTGACATGGCAGGGAGAAAATATACGTTTCTGGTGACGATATTGCTGATGGGCATATCGACTTTCACAAT
AGGTCTACTACCGGCCTATTCTACGATAGGCCTTGCGGCACCTCTTGGGTTTGTGGCGATGCGGATGCTGCAAGGCCTCG
CTCTTGGTGGAGAGTTCGGCGGTGCGCTAATCTATGTTGCCGAACACGCGCCTGCGAACCGAAGGGCGGCCTGGACGGCC
TGGGTGATATTGACGGCCGCGCTTGGATTTCTCTTGGCAGTCGCTGTCATCATTCCTCTAAGGCTGGCAATTGGCGCTGA
TGCCTTCTCTCTTTGGGGATGGCGCGCCCCCTTTCTTGTTTCAATCCTACTGCTCGGAGTTTCTTTGTGGATTCGATTGA
AATTGGACGAAACTCCCGAGTTCATAAGGATGAAGGCGGAGGGAAAGGCATCTAAAGCCCCAATCTCGGAAACGCTTGGA
ACGTGGAAAAACCTCCGCCTTGTGCTAATCGCTGCGCTCTGCATCGTTCCGGGGCAGGCGGTTGTATGGTACACTGGCCA
ATTCTACTCGTTGTTCTTTTTAACTAAAGTGTTGCGGATCGAAAATCTGACAGCAAATTTTCTGCTGATCGCTGCGACGA
TCATCACGGCCCCTCTTTACGTTGTCTTTGGTGCGTTGTCTGACCGTATCGGTCGCCGGCCAGTTTACGTGGCTGGTTTC
CTGCTTGCAGCTGTATTTACGGTCCCCCTTTTCAAAGCTCTTACGCACTACGGCAACCCGACACTCGAACAAGCGCAAAT
TAATGCGCCCATCACAATTGTATCAGGGAGTGACGCTTGTTCAGTACAATTCAATCCGCTGGGCACCGCAAAACCAATCA
CATCTTGCGACATCGTGGTCGACGCGATCGCAAAACTTGGTCTGAATTACAATAGTGCACACTCAGCAGAGTCAGCGACT
ACAATCGTGAAGATCGGCGACCGTGAGGTTCCTGGATACTCCGCCGATACATCCGACGTTTCCGTTAAAAAAACACGGTT
TGAATCGGAACTGAAGACAGCATTGACCGATATGGGCTATCCCTTAGGAGAAGCCGCCCATGAAGACATCAATCAAACTA
TGATCGTCGTTCTATTGTCCATCCTTTTATGCTTTGGAACGATGACGTTCGCGCCTTCGACAACTGCTCTACTCGAAATG
TTCCCTTCGCGGATACGGTATACTGCCATGTCATTTCCCTATCACCTAAGTGCAGCGTGGTTTGGTGGGTTCCTACCCGC
GACAGCGTTTGCCATCGTTGCGTCCACCGGCAACATTTATTCTGGGCTTTATTATCCGGCGTGCATCGCTGCAGCTTGTA
TAGTCTTGAGCACTATCTTTGCGAACGAGACAAAAGGAGCGGATCTCTCCGGAGATTGA

Upstream 100 bases:

>100_bases
CCCACGCCACCGCTTTTTGGCGTTTTGAAAAGCAAAATCAGGAACGTGGCAGAAATGCCGCGACGAACGACTGCGCTAAA
TATCGAACTGGGAGGAAATC

Downstream 100 bases:

>100_bases
TTGCGATTACCCGCGATCAAGCACTTCACGCCCGGTGAGATACGGCGCAGAGCACAATAAAGGCTTACGGAGCTATACAT
GAGAAGAGATTTTTATATGC

Product: major facilitator superfamily MFS_1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MVQVDILPKTEGISSNERRVIVAASLGTVFEFYDFFLIGLLANEISKAFFSGVNPTAGFIFTLLGFAAGFLLRPFGAIVF
GRLGDMAGRKYTFLVTILLMGISTFTIGLLPAYSTIGLAAPLGFVAMRMLQGLALGGEFGGALIYVAEHAPANRRAAWTA
WVILTAALGFLLAVAVIIPLRLAIGADAFSLWGWRAPFLVSILLLGVSLWIRLKLDETPEFIRMKAEGKASKAPISETLG
TWKNLRLVLIAALCIVPGQAVVWYTGQFYSLFFLTKVLRIENLTANFLLIAATIITAPLYVVFGALSDRIGRRPVYVAGF
LLAAVFTVPLFKALTHYGNPTLEQAQINAPITIVSGSDACSVQFNPLGTAKPITSCDIVVDAIAKLGLNYNSAHSAESAT
TIVKIGDREVPGYSADTSDVSVKKTRFESELKTALTDMGYPLGEAAHEDINQTMIVVLLSILLCFGTMTFAPSTTALLEM
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVASTGNIYSGLYYPACIAAACIVLSTIFANETKGADLSGD

Sequences:

>Translated_552_residues
MVQVDILPKTEGISSNERRVIVAASLGTVFEFYDFFLIGLLANEISKAFFSGVNPTAGFIFTLLGFAAGFLLRPFGAIVF
GRLGDMAGRKYTFLVTILLMGISTFTIGLLPAYSTIGLAAPLGFVAMRMLQGLALGGEFGGALIYVAEHAPANRRAAWTA
WVILTAALGFLLAVAVIIPLRLAIGADAFSLWGWRAPFLVSILLLGVSLWIRLKLDETPEFIRMKAEGKASKAPISETLG
TWKNLRLVLIAALCIVPGQAVVWYTGQFYSLFFLTKVLRIENLTANFLLIAATIITAPLYVVFGALSDRIGRRPVYVAGF
LLAAVFTVPLFKALTHYGNPTLEQAQINAPITIVSGSDACSVQFNPLGTAKPITSCDIVVDAIAKLGLNYNSAHSAESAT
TIVKIGDREVPGYSADTSDVSVKKTRFESELKTALTDMGYPLGEAAHEDINQTMIVVLLSILLCFGTMTFAPSTTALLEM
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVASTGNIYSGLYYPACIAAACIVLSTIFANETKGADLSGD
>Mature_552_residues
MVQVDILPKTEGISSNERRVIVAASLGTVFEFYDFFLIGLLANEISKAFFSGVNPTAGFIFTLLGFAAGFLLRPFGAIVF
GRLGDMAGRKYTFLVTILLMGISTFTIGLLPAYSTIGLAAPLGFVAMRMLQGLALGGEFGGALIYVAEHAPANRRAAWTA
WVILTAALGFLLAVAVIIPLRLAIGADAFSLWGWRAPFLVSILLLGVSLWIRLKLDETPEFIRMKAEGKASKAPISETLG
TWKNLRLVLIAALCIVPGQAVVWYTGQFYSLFFLTKVLRIENLTANFLLIAATIITAPLYVVFGALSDRIGRRPVYVAGF
LLAAVFTVPLFKALTHYGNPTLEQAQINAPITIVSGSDACSVQFNPLGTAKPITSCDIVVDAIAKLGLNYNSAHSAESAT
TIVKIGDREVPGYSADTSDVSVKKTRFESELKTALTDMGYPLGEAAHEDINQTMIVVLLSILLCFGTMTFAPSTTALLEM
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVASTGNIYSGLYYPACIAAACIVLSTIFANETKGADLSGD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=324, Percent_Identity=34.5679012345679, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788292, Length=327, Percent_Identity=34.8623853211009, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788942, Length=364, Percent_Identity=33.5164835164835, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789941, Length=333, Percent_Identity=35.1351351351351, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87082231, Length=232, Percent_Identity=28.448275862069, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87082404, Length=328, Percent_Identity=25, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59185; Mature: 59185

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVDILPKTEGISSNERRVIVAASLGTVFEFYDFFLIGLLANEISKAFFSGVNPTAGFI
CEEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FTLLGFAAGFLLRPFGAIVFGRLGDMAGRKYTFLVTILLMGISTFTIGLLPAYSTIGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLGFVAMRMLQGLALGGEFGGALIYVAEHAPANRRAAWTAWVILTAALGFLLAVAVIIPL
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLAIGADAFSLWGWRAPFLVSILLLGVSLWIRLKLDETPEFIRMKAEGKASKAPISETLG
HHHHCCCHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHEEEECCCCCCCCCHHHHHH
TWKNLRLVLIAALCIVPGQAVVWYTGQFYSLFFLTKVLRIENLTANFLLIAATIITAPLY
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VVFGALSDRIGRRPVYVAGFLLAAVFTVPLFKALTHYGNPTLEQAQINAPITIVSGSDAC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCEE
SVQFNPLGTAKPITSCDIVVDAIAKLGLNYNSAHSAESATTIVKIGDREVPGYSADTSDV
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
SVKKTRFESELKTALTDMGYPLGEAAHEDINQTMIVVLLSILLCFGTMTFAPSTTALLEM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVASTGNIYSGLYYPACIAAACIVLSTIF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHH
ANETKGADLSGD
HCCCCCCCCCCC
>Mature Secondary Structure
MVQVDILPKTEGISSNERRVIVAASLGTVFEFYDFFLIGLLANEISKAFFSGVNPTAGFI
CEEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FTLLGFAAGFLLRPFGAIVFGRLGDMAGRKYTFLVTILLMGISTFTIGLLPAYSTIGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLGFVAMRMLQGLALGGEFGGALIYVAEHAPANRRAAWTAWVILTAALGFLLAVAVIIPL
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLAIGADAFSLWGWRAPFLVSILLLGVSLWIRLKLDETPEFIRMKAEGKASKAPISETLG
HHHHCCCHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHEEEECCCCCCCCCHHHHHH
TWKNLRLVLIAALCIVPGQAVVWYTGQFYSLFFLTKVLRIENLTANFLLIAATIITAPLY
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VVFGALSDRIGRRPVYVAGFLLAAVFTVPLFKALTHYGNPTLEQAQINAPITIVSGSDAC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCEE
SVQFNPLGTAKPITSCDIVVDAIAKLGLNYNSAHSAESATTIVKIGDREVPGYSADTSDV
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
SVKKTRFESELKTALTDMGYPLGEAAHEDINQTMIVVLLSILLCFGTMTFAPSTTALLEM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVASTGNIYSGLYYPACIAAACIVLSTIF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHH
ANETKGADLSGD
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]