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The map label for this gene is lcfB [H]

Identifier: 209551963

GI number: 209551963

Start: 24966

End: 26504

Strand: Reverse

Name: lcfB [H]

Synonym: Rleg2_6098

Alternate gene names: 209551963

Gene position: 26504-24966 (Counterclockwise)

Preceding gene: 209551970

Following gene: 209551955

Centisome position: 8.58

GC content: 58.02

Gene sequence:

>1539_bases
ATGCAGGTATCGGAATACGAACTGAAATCACGGTTGAAACGTCACGACATCGCCGACCTGCTGCAATTCCCGCAGGAGGG
ATTTGGCGCGAAGACGTGTTTTCACTTCGAAGGCGTTGATCTGTCTTATCGGGAGCTTCGCCGTAGCTCACGCGCGCTGG
GCGCGGGTTTGATGTCCCGCGGATTGAAGCATCAGGAACGGGTAACGACACTTCTTCCGAACTGCCTGGAGATCTGGCAG
CTCTATTTCGCGACAGCCGCGGCGCGAGGCATCTTCATGCCTCTCAACCGCGATCTTGCTCCGTCTGAAATCGCATACAT
GCTGAACCATGCCTCTCCCCGGTTCATATTCACATGCTCCGCATTGCTGCCGAAGCTCGAAGAGGCGCTGGCGGCCCTGG
ACTATGAGCCGACGGTGATCATGATCGACGAAGCCAAACCCCATGGCCGGCGGGTCGACTTTCTGGAAGCGGCGGATGTC
GCTTCGCTGCCGGCCGTCGATCCCGAAGACGTGGTCATCATTTCCTACACGTCCGGCTCCACCTCCAATCCGAAGGCCAT
GGCGTTCTCGCATCGCGTGGAGTTGGACGGCGCGAAACTCTATGAGGACGCATGGGGCATCACGTCCAAGGACAAGGTCC
TGATCGCTATGTCGCTTGGCTGGACCTTCGGCGTCAATCCGGGGTCGTTTCCCGAGCTCCGCGCCGGCGGTTCGATTCTG
CTTCTCGAGAAATTCAATCCGGTAAAGGTGCTCGAGGCGATCGAAAAGCACGGGATTACCGTAATGAAGGGTGTGCCTAC
CATGTACGCCATGATGCTGGCACACGTCGAAGAGACTGGAAAGGAATACGACCTTTCGTCACTGCGCGTGGCCCTCTGTG
CCGGAGCGGATCTCCCTCTCACGCTTGCGCAGAAATTCAAACAGCGCTTTGGTCTGCAGCTCACGAACTTTCTTGGCATG
TCGGAGGTGAAGCTGGTTGCCTCGCCGCGTTGCGAGAACGATTTCTTTGCACCAGACGGCTCCGTGGGAACGCCTCCTCC
GGGCATGGAAGTTCGGTTCGTCGACGAGAACATGCAGGATGTGCCGACGGGGCAAACCGGCGAGTTTCTGGTGCGGTCCG
TCGCTTGGATGACAGGATACTTCGGCGACCCGGAGAAGACTGCAGCCTGTAACTTAGGCGGTTGGTACGTATCCGGGGAT
CTGGGGCGGCGAGACGAAGCCGGCTACATCTATTACGTGGGCCGACTACGCGAACAAATCATCCGCGGCGGCGCGAAAAT
TGCTCCGGCGGAAGTCGAGGAAGTGCTCATGTCGCGTCCCGATATCGCACTTTGTGCTGTCATTGGCGTGCCGGACGAGA
TGTACGGCGAAGCGGTAAAAGCGTTCATCGTCTCGAAAGACGCCCGGCTGGACACGGAGGCCCTAAAAGCCTACTGCGCC
GAAAGATTGGCAAACTTCAAGGTGCCGACGATATTCGAGTTTGTCCCTGATTTGCCGATTGGCCCGACAGGGAAGGTGCA
AAAGAAGCTGCTGAGATAA

Upstream 100 bases:

>100_bases
TAAGCTGGCTTGTTCGTATCTCGGGACCGAGCGGTGCGGCGATTCCGCGGCAAAGACCGAATTAGGGTCGTCAAGATTAA
TGGCATCACGGAGCGTTGAA

Downstream 100 bases:

>100_bases
TCTCGGTGTCTGCTATACGAAGCGGCTTTGCCCGGGTCGAGACTGCTTTCTAGCGCCTTGGCTAATTGTCACCAGCTGGG
GGATACTGCTGGGCGAGCGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQVSEYELKSRLKRHDIADLLQFPQEGFGAKTCFHFEGVDLSYRELRRSSRALGAGLMSRGLKHQERVTTLLPNCLEIWQ
LYFATAAARGIFMPLNRDLAPSEIAYMLNHASPRFIFTCSALLPKLEEALAALDYEPTVIMIDEAKPHGRRVDFLEAADV
ASLPAVDPEDVVIISYTSGSTSNPKAMAFSHRVELDGAKLYEDAWGITSKDKVLIAMSLGWTFGVNPGSFPELRAGGSIL
LLEKFNPVKVLEAIEKHGITVMKGVPTMYAMMLAHVEETGKEYDLSSLRVALCAGADLPLTLAQKFKQRFGLQLTNFLGM
SEVKLVASPRCENDFFAPDGSVGTPPPGMEVRFVDENMQDVPTGQTGEFLVRSVAWMTGYFGDPEKTAACNLGGWYVSGD
LGRRDEAGYIYYVGRLREQIIRGGAKIAPAEVEEVLMSRPDIALCAVIGVPDEMYGEAVKAFIVSKDARLDTEALKAYCA
ERLANFKVPTIFEFVPDLPIGPTGKVQKKLLR

Sequences:

>Translated_512_residues
MQVSEYELKSRLKRHDIADLLQFPQEGFGAKTCFHFEGVDLSYRELRRSSRALGAGLMSRGLKHQERVTTLLPNCLEIWQ
LYFATAAARGIFMPLNRDLAPSEIAYMLNHASPRFIFTCSALLPKLEEALAALDYEPTVIMIDEAKPHGRRVDFLEAADV
ASLPAVDPEDVVIISYTSGSTSNPKAMAFSHRVELDGAKLYEDAWGITSKDKVLIAMSLGWTFGVNPGSFPELRAGGSIL
LLEKFNPVKVLEAIEKHGITVMKGVPTMYAMMLAHVEETGKEYDLSSLRVALCAGADLPLTLAQKFKQRFGLQLTNFLGM
SEVKLVASPRCENDFFAPDGSVGTPPPGMEVRFVDENMQDVPTGQTGEFLVRSVAWMTGYFGDPEKTAACNLGGWYVSGD
LGRRDEAGYIYYVGRLREQIIRGGAKIAPAEVEEVLMSRPDIALCAVIGVPDEMYGEAVKAFIVSKDARLDTEALKAYCA
ERLANFKVPTIFEFVPDLPIGPTGKVQKKLLR
>Mature_512_residues
MQVSEYELKSRLKRHDIADLLQFPQEGFGAKTCFHFEGVDLSYRELRRSSRALGAGLMSRGLKHQERVTTLLPNCLEIWQ
LYFATAAARGIFMPLNRDLAPSEIAYMLNHASPRFIFTCSALLPKLEEALAALDYEPTVIMIDEAKPHGRRVDFLEAADV
ASLPAVDPEDVVIISYTSGSTSNPKAMAFSHRVELDGAKLYEDAWGITSKDKVLIAMSLGWTFGVNPGSFPELRAGGSIL
LLEKFNPVKVLEAIEKHGITVMKGVPTMYAMMLAHVEETGKEYDLSSLRVALCAGADLPLTLAQKFKQRFGLQLTNFLGM
SEVKLVASPRCENDFFAPDGSVGTPPPGMEVRFVDENMQDVPTGQTGEFLVRSVAWMTGYFGDPEKTAACNLGGWYVSGD
LGRRDEAGYIYYVGRLREQIIRGGAKIAPAEVEEVLMSRPDIALCAVIGVPDEMYGEAVKAFIVSKDARLDTEALKAYCA
ERLANFKVPTIFEFVPDLPIGPTGKVQKKLLR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=497, Percent_Identity=27.1629778672032, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI122937307, Length=494, Percent_Identity=28.3400809716599, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI157311624, Length=490, Percent_Identity=27.3469387755102, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI157311622, Length=490, Percent_Identity=27.3469387755102, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=27.7227722772277, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI156151445, Length=513, Percent_Identity=27.4853801169591, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI58082049, Length=490, Percent_Identity=26.9387755102041, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=28.6549707602339, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI187761345, Length=511, Percent_Identity=27.0058708414873, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI187761343, Length=511, Percent_Identity=27.0058708414873, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI28416953, Length=512, Percent_Identity=25.390625, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI13375727, Length=520, Percent_Identity=23.8461538461538, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI8923896, Length=378, Percent_Identity=24.6031746031746, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI253970489, Length=378, Percent_Identity=24.6031746031746, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42544134, Length=326, Percent_Identity=23.6196319018405, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI195947378, Length=376, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=27.4253731343284, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI145693145, Length=494, Percent_Identity=27.9352226720648, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1786810, Length=495, Percent_Identity=28.0808080808081, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=25.1020408163265, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1786801, Length=469, Percent_Identity=25.3731343283582, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=23.0769230769231, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789201, Length=506, Percent_Identity=25.098814229249, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786529, Length=519, Percent_Identity=24.2774566473988, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788595, Length=350, Percent_Identity=25.4285714285714, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=509, Percent_Identity=28.4872298624754, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71994694, Length=522, Percent_Identity=27.3946360153257, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71994703, Length=522, Percent_Identity=27.3946360153257, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71994690, Length=534, Percent_Identity=27.3408239700375, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17559526, Length=501, Percent_Identity=26.7465069860279, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17557194, Length=494, Percent_Identity=26.3157894736842, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538037, Length=497, Percent_Identity=26.7605633802817, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=478, Percent_Identity=26.3598326359833, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=497, Percent_Identity=26.7605633802817, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=431, Percent_Identity=26.4501160092807, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71996755, Length=497, Percent_Identity=24.5472837022133, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71985884, Length=362, Percent_Identity=28.4530386740331, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=429, Percent_Identity=24.9417249417249, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71982997, Length=562, Percent_Identity=24.1992882562278, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=562, Percent_Identity=24.0213523131673, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=351, Percent_Identity=29.6296296296296, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319264, Length=374, Percent_Identity=24.0641711229947, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=29.5880149812734, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355181, Length=510, Percent_Identity=26.2745098039216, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI21356947, Length=486, Percent_Identity=26.1316872427983, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI18859661, Length=501, Percent_Identity=25.5489021956088, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24656500, Length=489, Percent_Identity=22.9038854805726, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24653035, Length=487, Percent_Identity=23.6139630390144, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356441, Length=488, Percent_Identity=24.5901639344262, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161076582, Length=494, Percent_Identity=22.8744939271255, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24648260, Length=425, Percent_Identity=25.4117647058824, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21358303, Length=462, Percent_Identity=24.8917748917749, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648257, Length=502, Percent_Identity=25.4980079681275, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20130357, Length=491, Percent_Identity=22.6069246435845, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648253, Length=443, Percent_Identity=23.4762979683973, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648255, Length=443, Percent_Identity=23.7020316027088, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667955, Length=550, Percent_Identity=22.9090909090909, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI62472339, Length=527, Percent_Identity=23.1499051233397, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281365686, Length=398, Percent_Identity=23.8693467336683, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922652, Length=491, Percent_Identity=21.9959266802444, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24667959, Length=379, Percent_Identity=25.5936675461741, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355225, Length=524, Percent_Identity=21.1832061068702, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648676, Length=516, Percent_Identity=23.062015503876, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221329755, Length=398, Percent_Identity=20.6030150753769, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56454; Mature: 56454

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSEYELKSRLKRHDIADLLQFPQEGFGAKTCFHFEGVDLSYRELRRSSRALGAGLMSR
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GLKHQERVTTLLPNCLEIWQLYFATAAARGIFMPLNRDLAPSEIAYMLNHASPRFIFTCS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCEEEEHH
ALLPKLEEALAALDYEPTVIMIDEAKPHGRRVDFLEAADVASLPAVDPEDVVIISYTSGS
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCCC
TSNPKAMAFSHRVELDGAKLYEDAWGITSKDKVLIAMSLGWTFGVNPGSFPELRAGGSIL
CCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCEEEEEEECEEECCCCCCCCHHCCCCCEE
LLEKFNPVKVLEAIEKHGITVMKGVPTMYAMMLAHVEETGKEYDLSSLRVALCAGADLPL
EEECCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCCH
TLAQKFKQRFGLQLTNFLGMSEVKLVASPRCENDFFAPDGSVGTPPPGMEVRFVDENMQD
HHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
VPTGQTGEFLVRSVAWMTGYFGDPEKTAACNLGGWYVSGDLGRRDEAGYIYYVGRLREQI
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCCCCCCCCEEEEHHHHHHHH
IRGGAKIAPAEVEEVLMSRPDIALCAVIGVPDEMYGEAVKAFIVSKDARLDTEALKAYCA
HHCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ERLANFKVPTIFEFVPDLPIGPTGKVQKKLLR
HHHHCCCCCHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MQVSEYELKSRLKRHDIADLLQFPQEGFGAKTCFHFEGVDLSYRELRRSSRALGAGLMSR
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GLKHQERVTTLLPNCLEIWQLYFATAAARGIFMPLNRDLAPSEIAYMLNHASPRFIFTCS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCEEEEHH
ALLPKLEEALAALDYEPTVIMIDEAKPHGRRVDFLEAADVASLPAVDPEDVVIISYTSGS
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCCC
TSNPKAMAFSHRVELDGAKLYEDAWGITSKDKVLIAMSLGWTFGVNPGSFPELRAGGSIL
CCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCEEEEEEECEEECCCCCCCCHHCCCCCEE
LLEKFNPVKVLEAIEKHGITVMKGVPTMYAMMLAHVEETGKEYDLSSLRVALCAGADLPL
EEECCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCCH
TLAQKFKQRFGLQLTNFLGMSEVKLVASPRCENDFFAPDGSVGTPPPGMEVRFVDENMQD
HHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
VPTGQTGEFLVRSVAWMTGYFGDPEKTAACNLGGWYVSGDLGRRDEAGYIYYVGRLREQI
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCCCCCCCCEEEEHHHHHHHH
IRGGAKIAPAEVEEVLMSRPDIALCAVIGVPDEMYGEAVKAFIVSKDARLDTEALKAYCA
HHCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ERLANFKVPTIFEFVPDLPIGPTGKVQKKLLR
HHHHCCCCCHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]