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The map label for this gene is potH [H]

Identifier: 209550736

GI number: 209550736

Start: 3243265

End: 3244092

Strand: Direct

Name: potH [H]

Synonym: Rleg2_3160

Alternate gene names: 209550736

Gene position: 3243265-3244092 (Clockwise)

Preceding gene: 209550735

Following gene: 209550737

Centisome position: 71.47

GC content: 62.92

Gene sequence:

>828_bases
ATGTTCCAGAACCGCGCAGAAGCCCTGGCGCTCGCCTTGCCGGCAGCGGTCTTTGCAACCGTGGTCTTCCTGCTGCCCGT
CGCGGTCCTGCTGTCGGAGGGCTTCCGTGCCGGCGGCGGCTGGACACTGTCGGCCTATGCCGGTTTCTTCTCCGAGACGC
TGAACCGCACCGTTTTCCTGCGCAGCTTCCGGCTCGGCCTCGAAGTCACCGCCGTCTCGGCGGTCATCGGTTATGCCGCA
GCCTTTGCCATCGTCAATCTGCCGCCGAATGGCAAGGGCCGCATGATCGGCCTCGTCACCCTGCCGCTGATGATCTCTCC
GGTGGCGCGCACCTATGCCTGGATCGTCATCCTCGGCCGCACCGGCATCGTCAATCAGGCGATAACGGGCCTCGGTCTCA
GCGCCGAGCCGCTGCGGCTGCTGTTCACCGAAACCGCCGTCTTCATCGGCCTCCTGCAGCTCTTTCTGCCGCTGATGATC
CTTTCCTTGATCAGTGCGCTCGAAAACATGCCGAAGGATGCGATCCCCGCTGCCCGCGTTCTCGGCGCCAACTGGTTCCA
GGTCTTCTGGAAGGTCATCCTGCCGCTGACCAGGGAAGGCCTCGTCGTCGGTGGCACGCTTGTCTTCACCGGATCGCTGA
CCGCCTATATCACGCCCGCCGTGCTTGGCGGCTCCAAGGTGCTGATGCTGGAAACGCTGCTATATCAGCAGGTTTCCGTC
GCCAATGATTTCGTCTCGGCAAGCGTCATCGCCTTCATCCTGATCATCATGAGCTTTGCCGCCAACATCCTGCTGAAACG
CCTTGCGACGGCGAGGAACCGCCGATGA

Upstream 100 bases:

>100_bases
CTGAAGACCTGGAACGAAAAGGTCGGCCAGTAAGGCCTGCGTGAGAACCGGTCCCGGCGCCTCTGCCGGGACCATCCTCT
GTCCGCTCGAAAGGCGCCTC

Downstream 100 bases:

>100_bases
CCCTGCGCCTGTTTTCGCCCGTGGTGCTTTTCCTGCTGCTGGTCTTTCTGATCGGCCCGTTCCTGATCATCATCGCCGCT
TCGCTGTCATCAGGCGATAC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MFQNRAEALALALPAAVFATVVFLLPVAVLLSEGFRAGGGWTLSAYAGFFSETLNRTVFLRSFRLGLEVTAVSAVIGYAA
AFAIVNLPPNGKGRMIGLVTLPLMISPVARTYAWIVILGRTGIVNQAITGLGLSAEPLRLLFTETAVFIGLLQLFLPLMI
LSLISALENMPKDAIPAARVLGANWFQVFWKVILPLTREGLVVGGTLVFTGSLTAYITPAVLGGSKVLMLETLLYQQVSV
ANDFVSASVIAFILIIMSFAANILLKRLATARNRR

Sequences:

>Translated_275_residues
MFQNRAEALALALPAAVFATVVFLLPVAVLLSEGFRAGGGWTLSAYAGFFSETLNRTVFLRSFRLGLEVTAVSAVIGYAA
AFAIVNLPPNGKGRMIGLVTLPLMISPVARTYAWIVILGRTGIVNQAITGLGLSAEPLRLLFTETAVFIGLLQLFLPLMI
LSLISALENMPKDAIPAARVLGANWFQVFWKVILPLTREGLVVGGTLVFTGSLTAYITPAVLGGSKVLMLETLLYQQVSV
ANDFVSASVIAFILIIMSFAANILLKRLATARNRR
>Mature_275_residues
MFQNRAEALALALPAAVFATVVFLLPVAVLLSEGFRAGGGWTLSAYAGFFSETLNRTVFLRSFRLGLEVTAVSAVIGYAA
AFAIVNLPPNGKGRMIGLVTLPLMISPVARTYAWIVILGRTGIVNQAITGLGLSAEPLRLLFTETAVFIGLLQLFLPLMI
LSLISALENMPKDAIPAARVLGANWFQVFWKVILPLTREGLVVGGTLVFTGSLTAYITPAVLGGSKVLMLETLLYQQVSV
ANDFVSASVIAFILIIMSFAANILLKRLATARNRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=225, Percent_Identity=31.1111111111111, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787369, Length=203, Percent_Identity=30.5418719211823, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787713, Length=233, Percent_Identity=29.1845493562232, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29457; Mature: 29457

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQNRAEALALALPAAVFATVVFLLPVAVLLSEGFRAGGGWTLSAYAGFFSETLNRTVFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHH
RSFRLGLEVTAVSAVIGYAAAFAIVNLPPNGKGRMIGLVTLPLMISPVARTYAWIVILGR
HHHHHCHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEHHHHHHHHHHHHHEEHEEECC
TGIVNQAITGLGLSAEPLRLLFTETAVFIGLLQLFLPLMILSLISALENMPKDAIPAARV
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LGANWFQVFWKVILPLTREGLVVGGTLVFTGSLTAYITPAVLGGSKVLMLETLLYQQVSV
HCCHHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ANDFVSASVIAFILIIMSFAANILLKRLATARNRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFQNRAEALALALPAAVFATVVFLLPVAVLLSEGFRAGGGWTLSAYAGFFSETLNRTVFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHH
RSFRLGLEVTAVSAVIGYAAAFAIVNLPPNGKGRMIGLVTLPLMISPVARTYAWIVILGR
HHHHHCHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEHHHHHHHHHHHHHEEHEEECC
TGIVNQAITGLGLSAEPLRLLFTETAVFIGLLQLFLPLMILSLISALENMPKDAIPAARV
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LGANWFQVFWKVILPLTREGLVVGGTLVFTGSLTAYITPAVLGGSKVLMLETLLYQQVSV
HCCHHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ANDFVSASVIAFILIIMSFAANILLKRLATARNRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]