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The map label for this gene is potD [H]

Identifier: 209550735

GI number: 209550735

Start: 3242175

End: 3243197

Strand: Direct

Name: potD [H]

Synonym: Rleg2_3159

Alternate gene names: 209550735

Gene position: 3242175-3243197 (Clockwise)

Preceding gene: 209550734

Following gene: 209550736

Centisome position: 71.45

GC content: 60.7

Gene sequence:

>1023_bases
ATGAAAAAAGCGCTCATTGCCTCGGCAGCACTCGCCGCCCTGTCTCCGCTCGGAGCTACAGCAGCGGACCGCACATTGAC
CATTTCGGTCTATGCTTTTGCCCAGGACGATTTCAAGACGCTGGTCTATGATCCCTTCGAAGCCAAATGCGGCTGCAAGC
TGGTGGTCGAGACCGGCAACAGCGTCGAACGCCTGGCCAAGATGGAAGCGAACAAGGCGAACCCCGTCGTCGACCTCGCC
GCTGTTTCCATGGCCGATGCGCTGGCCGCCTCCCGTGCCGGCCTGATCGACAAGGTCGACACCACCAAGCTCGCCAATTT
CACCAAGCTCTACGACGTCGCCAAGGATCCGAACGGCGACGGCATGAGCGTCGGTTACACCTTCTACGCCACCTCGATCG
CCTATCGCTCCGACAAGATGAAGATCGACTCCTGGGCCGATCTCCTGAAGCCGGAATATGTCGGCCACGTCGCCTTCCCG
AACGTGACGACCAACCAGGGGCCGCCGGCGCTCTATATGCTGGGCCAGGCGCTCGGCAAGGACACCCCCGATCTGAAGGG
GCCGATCGAGGCGCTGGGCGAGAAGAAGGACGACATCGTCACCTTCTACGAAAAATCCTCGCAGCTCGTGCAACTGATGC
AGCAGGAGGAAATCTGGGCCGCGCCGATCGGCCGTTTCTCCTGGGCTGGTTTTACCAAGCTCGATGTTCCGGTCGCCTGG
GCGACACCGAAAGAGGGTCAGACCGGCGGCATGAATGTGCTGGTGCTGACCAAGGGTTCGAAGAACCAGGATCTCGCCCT
GCAGTTCATGGATTTCTGGCTCTCGACCGACATCCAGACCAAACTCGCCGAAAAGCTGGTCGACAGCCCGGCCAACAGCG
AGGTCAAGCTTTCCGAAGCCGCTGCCAACAACCTCACCTATGGCGAGGAAACCGCCAAGAGCCTCAAGCTGATCCCTTCG
GCCGTCGCCCTCGACAATCGCGCCGGCTGGCTGAAGACCTGGAACGAAAAGGTCGGCCAGTAA

Upstream 100 bases:

>100_bases
GCCGAGATCGACCGTGTCCCCATGCAGCTGATCGTCCGCCAATCATCCGGGCCTTGCGAACCCGGCACCAAAAAAACCTC
AGAGATGGAGAACATGTAAG

Downstream 100 bases:

>100_bases
GGCCTGCGTGAGAACCGGTCCCGGCGCCTCTGCCGGGACCATCCTCTGTCCGCTCGAAAGGCGCCTCATGTTCCAGAACC
GCGCAGAAGCCCTGGCGCTC

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKALIASAALAALSPLGATAADRTLTISVYAFAQDDFKTLVYDPFEAKCGCKLVVETGNSVERLAKMEANKANPVVDLA
AVSMADALAASRAGLIDKVDTTKLANFTKLYDVAKDPNGDGMSVGYTFYATSIAYRSDKMKIDSWADLLKPEYVGHVAFP
NVTTNQGPPALYMLGQALGKDTPDLKGPIEALGEKKDDIVTFYEKSSQLVQLMQQEEIWAAPIGRFSWAGFTKLDVPVAW
ATPKEGQTGGMNVLVLTKGSKNQDLALQFMDFWLSTDIQTKLAEKLVDSPANSEVKLSEAAANNLTYGEETAKSLKLIPS
AVALDNRAGWLKTWNEKVGQ

Sequences:

>Translated_340_residues
MKKALIASAALAALSPLGATAADRTLTISVYAFAQDDFKTLVYDPFEAKCGCKLVVETGNSVERLAKMEANKANPVVDLA
AVSMADALAASRAGLIDKVDTTKLANFTKLYDVAKDPNGDGMSVGYTFYATSIAYRSDKMKIDSWADLLKPEYVGHVAFP
NVTTNQGPPALYMLGQALGKDTPDLKGPIEALGEKKDDIVTFYEKSSQLVQLMQQEEIWAAPIGRFSWAGFTKLDVPVAW
ATPKEGQTGGMNVLVLTKGSKNQDLALQFMDFWLSTDIQTKLAEKLVDSPANSEVKLSEAAANNLTYGEETAKSLKLIPS
AVALDNRAGWLKTWNEKVGQ
>Mature_340_residues
MKKALIASAALAALSPLGATAADRTLTISVYAFAQDDFKTLVYDPFEAKCGCKLVVETGNSVERLAKMEANKANPVVDLA
AVSMADALAASRAGLIDKVDTTKLANFTKLYDVAKDPNGDGMSVGYTFYATSIAYRSDKMKIDSWADLLKPEYVGHVAFP
NVTTNQGPPALYMLGQALGKDTPDLKGPIEALGEKKDDIVTFYEKSSQLVQLMQQEEIWAAPIGRFSWAGFTKLDVPVAW
ATPKEGQTGGMNVLVLTKGSKNQDLALQFMDFWLSTDIQTKLAEKLVDSPANSEVKLSEAAANNLTYGEETAKSLKLIPS
AVALDNRAGWLKTWNEKVGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=279, Percent_Identity=26.5232974910394, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787078, Length=317, Percent_Identity=26.813880126183, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36717; Mature: 36717

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALIASAALAALSPLGATAADRTLTISVYAFAQDDFKTLVYDPFEAKCGCKLVVETGN
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCCEEEEECCC
SVERLAKMEANKANPVVDLAAVSMADALAASRAGLIDKVDTTKLANFTKLYDVAKDPNGD
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GMSVGYTFYATSIAYRSDKMKIDSWADLLKPEYVGHVAFPNVTTNQGPPALYMLGQALGK
CEEEEEEEEEEEEEECCCCEEHHHHHHHHCHHHCCEEECCCCCCCCCCCHHHHHHHHHCC
DTPDLKGPIEALGEKKDDIVTFYEKSSQLVQLMQQEEIWAAPIGRFSWAGFTKLDVPVAW
CCCCCCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEE
ATPKEGQTGGMNVLVLTKGSKNQDLALQFMDFWLSTDIQTKLAEKLVDSPANSEVKLSEA
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEHHH
AANNLTYGEETAKSLKLIPSAVALDNRAGWLKTWNEKVGQ
HCCCCCCCHHHHHHHHHCHHHHEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKALIASAALAALSPLGATAADRTLTISVYAFAQDDFKTLVYDPFEAKCGCKLVVETGN
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCCEEEEECCC
SVERLAKMEANKANPVVDLAAVSMADALAASRAGLIDKVDTTKLANFTKLYDVAKDPNGD
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GMSVGYTFYATSIAYRSDKMKIDSWADLLKPEYVGHVAFPNVTTNQGPPALYMLGQALGK
CEEEEEEEEEEEEEECCCCEEHHHHHHHHCHHHCCEEECCCCCCCCCCCHHHHHHHHHCC
DTPDLKGPIEALGEKKDDIVTFYEKSSQLVQLMQQEEIWAAPIGRFSWAGFTKLDVPVAW
CCCCCCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEE
ATPKEGQTGGMNVLVLTKGSKNQDLALQFMDFWLSTDIQTKLAEKLVDSPANSEVKLSEA
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEHHH
AANNLTYGEETAKSLKLIPSAVALDNRAGWLKTWNEKVGQ
HCCCCCCCHHHHHHHHHCHHHHEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]