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The map label for this gene is degA [H]

Identifier: 209550734

GI number: 209550734

Start: 3241127

End: 3242173

Strand: Direct

Name: degA [H]

Synonym: Rleg2_3158

Alternate gene names: 209550734

Gene position: 3241127-3242173 (Clockwise)

Preceding gene: 209550733

Following gene: 209550735

Centisome position: 71.42

GC content: 63.99

Gene sequence:

>1047_bases
ATGGCTTCATCGCGTTCGGGACCGAACCTCAGCAGGATAGCGACGTCGCTCGGCGTCTCCGTCGCCACCGTCTCCAATGC
ACTATCAGGCAAGGGCCGCGTCTCCGGCCAACTGGTCGAAAGGATTCGCGAGCATGCCGCCGAGCTCGGTTATGTCCCGA
GCCAAGCCGGGCGAGCGCTCAGAACCGGCCGCAGCGGCGTTCTCGGCCTGGTGCTGCCCGATATCGCCAATCCGCTGTTC
CCGAAAATCGCCCAGGCGATCGAATTCGCCGCTTCTGCCGCCGGTTACGGCGTGCTGATCGCTGATTCCCGCGGCGATGT
CACCGCCCAGACCGAGGCGATCAATCGGCTGGTCGAGCGCGGCGTCGACGGCATGGTCGTCATTCCCCGCCGCGCCACCC
GCATCTCTTCCGCCGCCTGTCCCATAGCCGTCATCGATACCCCATCGACGTCAGGCAACACCGTTGCCGCCGACCATTGG
CAGGGCGGCTGCGAAATCGCCGGCCATCTCGCCGGCCTTGGCCATCGCCGCATCCTCATCATCGGCAATAATCAGGAATC
CAGCGTCCAGAACGACCGCGCCGATGGCATCCACTCCGGCATGCGTCCGGACATGCATGCGGAAACGCTGTGGATCGACA
GGCTCGAGCAGGATAGCGGCATCGGCTGCCCGCTCGGTCTCGCCGAAAAAGTCAGGCAGGGTTTCACCGCCTTCGCAGCC
CTCTCCGACCTGCAGGCGCTGCGGGCGCTGACGGAGTTGCAGCAGGCCGGCGTCAACATTCCCGTCGATGTCAGCGTCAC
CGGCTTCGACGATCTCATCTGGTCGCCGGTCGTCACACCGTCGCTGACGACTGTTCGTATGGACATGGACGCGATTGCCG
GGATTGCCGTGTCGGCACTGGTGGATACCATAAGAGCGAACAGCATCCGGCAGGGCATGCTGGTGACCGCCGAGATCGAC
CGTGTCCCCATGCAGCTGATCGTCCGCCAATCATCCGGGCCTTGCGAACCCGGCACCAAAAAAACCTCAGAGATGGAGAA
CATGTAA

Upstream 100 bases:

>100_bases
TGTATCGCGGATAATGCGCGATAAGATGAATTGGGAATCGACAAGCGAAAAGAGCTGAGGTAAAGCTCTGATTAAACGAT
TAATCAGAGCGCCTTCGATC

Downstream 100 bases:

>100_bases
GATGAAAAAAGCGCTCATTGCCTCGGCAGCACTCGCCGCCCTGTCTCCGCTCGGAGCTACAGCAGCGGACCGCACATTGA
CCATTTCGGTCTATGCTTTT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MASSRSGPNLSRIATSLGVSVATVSNALSGKGRVSGQLVERIREHAAELGYVPSQAGRALRTGRSGVLGLVLPDIANPLF
PKIAQAIEFAASAAGYGVLIADSRGDVTAQTEAINRLVERGVDGMVVIPRRATRISSAACPIAVIDTPSTSGNTVAADHW
QGGCEIAGHLAGLGHRRILIIGNNQESSVQNDRADGIHSGMRPDMHAETLWIDRLEQDSGIGCPLGLAEKVRQGFTAFAA
LSDLQALRALTELQQAGVNIPVDVSVTGFDDLIWSPVVTPSLTTVRMDMDAIAGIAVSALVDTIRANSIRQGMLVTAEID
RVPMQLIVRQSSGPCEPGTKKTSEMENM

Sequences:

>Translated_348_residues
MASSRSGPNLSRIATSLGVSVATVSNALSGKGRVSGQLVERIREHAAELGYVPSQAGRALRTGRSGVLGLVLPDIANPLF
PKIAQAIEFAASAAGYGVLIADSRGDVTAQTEAINRLVERGVDGMVVIPRRATRISSAACPIAVIDTPSTSGNTVAADHW
QGGCEIAGHLAGLGHRRILIIGNNQESSVQNDRADGIHSGMRPDMHAETLWIDRLEQDSGIGCPLGLAEKVRQGFTAFAA
LSDLQALRALTELQQAGVNIPVDVSVTGFDDLIWSPVVTPSLTTVRMDMDAIAGIAVSALVDTIRANSIRQGMLVTAEID
RVPMQLIVRQSSGPCEPGTKKTSEMENM
>Mature_347_residues
ASSRSGPNLSRIATSLGVSVATVSNALSGKGRVSGQLVERIREHAAELGYVPSQAGRALRTGRSGVLGLVLPDIANPLFP
KIAQAIEFAASAAGYGVLIADSRGDVTAQTEAINRLVERGVDGMVVIPRRATRISSAACPIAVIDTPSTSGNTVAADHWQ
GGCEIAGHLAGLGHRRILIIGNNQESSVQNDRADGIHSGMRPDMHAETLWIDRLEQDSGIGCPLGLAEKVRQGFTAFAAL
SDLQALRALTELQQAGVNIPVDVSVTGFDDLIWSPVVTPSLTTVRMDMDAIAGIAVSALVDTIRANSIRQGMLVTAEIDR
VPMQLIVRQSSGPCEPGTKKTSEMENM

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=313, Percent_Identity=27.4760383386581, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=22.884012539185, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=28.2312925170068, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=30, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=24.5398773006135, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787948, Length=304, Percent_Identity=24.0131578947368, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=27.6872964169381, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787906, Length=195, Percent_Identity=31.7948717948718, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786540, Length=349, Percent_Identity=24.3553008595989, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=25.0793650793651, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786268, Length=209, Percent_Identity=30.622009569378, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36558; Mature: 36427

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRSGPNLSRIATSLGVSVATVSNALSGKGRVSGQLVERIREHAAELGYVPSQAGRAL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
RTGRSGVLGLVLPDIANPLFPKIAQAIEFAASAAGYGVLIADSRGDVTAQTEAINRLVER
HCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHC
GVDGMVVIPRRATRISSAACPIAVIDTPSTSGNTVAADHWQGGCEIAGHLAGLGHRRILI
CCCCEEECCCCHHHHHCCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEE
IGNNQESSVQNDRADGIHSGMRPDMHAETLWIDRLEQDSGIGCPLGLAEKVRQGFTAFAA
EECCCCHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LSDLQALRALTELQQAGVNIPVDVSVTGFDDLIWSPVVTPSLTTVRMDMDAIAGIAVSAL
HHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHH
VDTIRANSIRQGMLVTAEIDRVPMQLIVRQSSGPCEPGTKKTSEMENM
HHHHHHHHHHCCEEEEEEHHHCCHHHEEECCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
ASSRSGPNLSRIATSLGVSVATVSNALSGKGRVSGQLVERIREHAAELGYVPSQAGRAL
CCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
RTGRSGVLGLVLPDIANPLFPKIAQAIEFAASAAGYGVLIADSRGDVTAQTEAINRLVER
HCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHC
GVDGMVVIPRRATRISSAACPIAVIDTPSTSGNTVAADHWQGGCEIAGHLAGLGHRRILI
CCCCEEECCCCHHHHHCCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEE
IGNNQESSVQNDRADGIHSGMRPDMHAETLWIDRLEQDSGIGCPLGLAEKVRQGFTAFAA
EECCCCHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LSDLQALRALTELQQAGVNIPVDVSVTGFDDLIWSPVVTPSLTTVRMDMDAIAGIAVSAL
HHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHH
VDTIRANSIRQGMLVTAEIDRVPMQLIVRQSSGPCEPGTKKTSEMENM
HHHHHHHHHHCCEEEEEEHHHCCHHHEEECCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]