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The map label for this gene is bepF [H]

Identifier: 209550683

GI number: 209550683

Start: 3174438

End: 3175616

Strand: Direct

Name: bepF [H]

Synonym: Rleg2_3107

Alternate gene names: 209550683

Gene position: 3174438-3175616 (Clockwise)

Preceding gene: 209550682

Following gene: 209550684

Centisome position: 69.95

GC content: 63.7

Gene sequence:

>1179_bases
ATGACTTACGTGCTTCGACGGATATTGATGCTCGGTTGCATCACCACGTTCATGCCCCTGGCCGCCCTGGCGCAGGGCGC
ACCGTCTGCCCCTCCTCCCGTCACCGTCGCCAAGCCGGTCGTTCGCGATGTCGTCGACAGCGACGAGTTCATCGGCCGCT
TCGAGCCGGTCGACGAAGTCTCGGTTCGCTCGCGTGTCGGTGGGTACCTGCAGGAAATCCATTTCCAGGACGGCGCATTG
GTCAAGCAGGGTGACCTGCTGTTCGTCATCGACCAGAGGCCGTTCGTAACCGCGCTCAGCCAGGCAAAGGCGACGCTCGA
AGCGTCGCAATCCGCTCTGGTCTTTGCCGATGCGCAGTTCAAACGCGCACAATCGCTCACCGCGAGCGGCAGCCAGGCGG
CGCAGACGCTGGACGATCGCCGCCGCGAGTTCGATTCGGCCGAGGCCAATGTCCGCGGCGCGCAGGCAGCCGCCGATCGC
GCTTCTCTCGACATGGAATATACCGAGATCAAAGCGCCGCTCAGCGGCCGCATCGACCGCCGGCTGATCTCGGCCGGCAA
TCTGGTGCAGACCGACCAGACCGTGCTGACCACCATCGTCTCGCTCGACCCGATCGATTTCTATTTCGACGTCGATGAGC
GCCGCCTGCTGAATTTCGCCGACACGGCGCGCAAAATGGGTAAGGAACTTCAGCAGGGCGGCGGCGGATTGGATGTATCC
GTCACCATCTCGGATCCTTCTGCCAAGCCATTCAAGGGGAAACTCGACTTCGCCGAGAACCGGGTCGACAATCAGAGCGG
CACCATTCGTCTGCGCGCCCGCTTCCCCAACCCCGATCTCGTCCTTCAGCCCGGCCTCTTCGGCCGCATTCAGGTCGAAG
CCTCCAACACCTACCAGGCTATTCTCGTCCCGGACGAGGCAATCGGCTCGGACCAGAATGAACGCGTCGTCTATGTGGTT
GACGCGCAAGGAAAAGTTTCGACCAAGCCCGTGAGGCCCGGCCCGCGGCTCTATGGCTATCGTGTGATCCGCGAGGGCCT
CGACGGCACCGAGACGATCATCGTCAACGGCCTGATGCGCGCCCGCCCGGGATCGACGATCACGCCGCAGATGACCGAAC
TGCCGCGGGAACGCCAGGACACCCCGCCCGAGGCCGCCAATGCGGAGAGCGGACAATGA

Upstream 100 bases:

>100_bases
GGGGGCCACGCCTTCGTTCAAAGCAGCAAAACATGAAATAGCATCACCTTATCTTAGGTTCGGCGGCAGTCTATCGGCCG
TCGCGATTGGAGGGACCGGT

Downstream 100 bases:

>100_bases
GATTCGCGCACTTCTTCGTTGACCGGCCGATCTTTGCGGCCGTCATCTCGATCCTCTTTCTCGTCGTCGGCGGCATCGCC
TACACACAGTTGCCGGTCTC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTYVLRRILMLGCITTFMPLAALAQGAPSAPPPVTVAKPVVRDVVDSDEFIGRFEPVDEVSVRSRVGGYLQEIHFQDGAL
VKQGDLLFVIDQRPFVTALSQAKATLEASQSALVFADAQFKRAQSLTASGSQAAQTLDDRRREFDSAEANVRGAQAAADR
ASLDMEYTEIKAPLSGRIDRRLISAGNLVQTDQTVLTTIVSLDPIDFYFDVDERRLLNFADTARKMGKELQQGGGGLDVS
VTISDPSAKPFKGKLDFAENRVDNQSGTIRLRARFPNPDLVLQPGLFGRIQVEASNTYQAILVPDEAIGSDQNERVVYVV
DAQGKVSTKPVRPGPRLYGYRVIREGLDGTETIIVNGLMRARPGSTITPQMTELPRERQDTPPEAANAESGQ

Sequences:

>Translated_392_residues
MTYVLRRILMLGCITTFMPLAALAQGAPSAPPPVTVAKPVVRDVVDSDEFIGRFEPVDEVSVRSRVGGYLQEIHFQDGAL
VKQGDLLFVIDQRPFVTALSQAKATLEASQSALVFADAQFKRAQSLTASGSQAAQTLDDRRREFDSAEANVRGAQAAADR
ASLDMEYTEIKAPLSGRIDRRLISAGNLVQTDQTVLTTIVSLDPIDFYFDVDERRLLNFADTARKMGKELQQGGGGLDVS
VTISDPSAKPFKGKLDFAENRVDNQSGTIRLRARFPNPDLVLQPGLFGRIQVEASNTYQAILVPDEAIGSDQNERVVYVV
DAQGKVSTKPVRPGPRLYGYRVIREGLDGTETIIVNGLMRARPGSTITPQMTELPRERQDTPPEAANAESGQ
>Mature_391_residues
TYVLRRILMLGCITTFMPLAALAQGAPSAPPPVTVAKPVVRDVVDSDEFIGRFEPVDEVSVRSRVGGYLQEIHFQDGALV
KQGDLLFVIDQRPFVTALSQAKATLEASQSALVFADAQFKRAQSLTASGSQAAQTLDDRRREFDSAEANVRGAQAAADRA
SLDMEYTEIKAPLSGRIDRRLISAGNLVQTDQTVLTTIVSLDPIDFYFDVDERRLLNFADTARKMGKELQQGGGGLDVSV
TISDPSAKPFKGKLDFAENRVDNQSGTIRLRARFPNPDLVLQPGLFGRIQVEASNTYQAILVPDEAIGSDQNERVVYVVD
AQGKVSTKPVRPGPRLYGYRVIREGLDGTETIIVNGLMRARPGSTITPQMTELPRERQDTPPEAANAESGQ

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=361, Percent_Identity=29.0858725761773, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789929, Length=366, Percent_Identity=28.6885245901639, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786668, Length=327, Percent_Identity=32.1100917431193, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789665, Length=353, Percent_Identity=29.1784702549575, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081951, Length=151, Percent_Identity=31.1258278145695, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42751; Mature: 42620

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYVLRRILMLGCITTFMPLAALAQGAPSAPPPVTVAKPVVRDVVDSDEFIGRFEPVDEV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCHHHH
SVRSRVGGYLQEIHFQDGALVKQGDLLFVIDQRPFVTALSQAKATLEASQSALVFADAQF
HHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHH
KRAQSLTASGSQAAQTLDDRRREFDSAEANVRGAQAAADRASLDMEYTEIKAPLSGRIDR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCHHH
RLISAGNLVQTDQTVLTTIVSLDPIDFYFDVDERRLLNFADTARKMGKELQQGGGGLDVS
HHHCCCCEEECCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VTISDPSAKPFKGKLDFAENRVDNQSGTIRLRARFPNPDLVLQPGLFGRIQVEASNTYQA
EEECCCCCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCEEECCCCCEEEEEEECCCEEE
ILVPDEAIGSDQNERVVYVVDAQGKVSTKPVRPGPRLYGYRVIREGLDGTETIIVNGLMR
EEECHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECHHH
ARPGSTITPQMTELPRERQDTPPEAANAESGQ
CCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYVLRRILMLGCITTFMPLAALAQGAPSAPPPVTVAKPVVRDVVDSDEFIGRFEPVDEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCHHHH
SVRSRVGGYLQEIHFQDGALVKQGDLLFVIDQRPFVTALSQAKATLEASQSALVFADAQF
HHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHH
KRAQSLTASGSQAAQTLDDRRREFDSAEANVRGAQAAADRASLDMEYTEIKAPLSGRIDR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCHHH
RLISAGNLVQTDQTVLTTIVSLDPIDFYFDVDERRLLNFADTARKMGKELQQGGGGLDVS
HHHCCCCEEECCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VTISDPSAKPFKGKLDFAENRVDNQSGTIRLRARFPNPDLVLQPGLFGRIQVEASNTYQA
EEECCCCCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCEEECCCCCEEEEEEECCCEEE
ILVPDEAIGSDQNERVVYVVDAQGKVSTKPVRPGPRLYGYRVIREGLDGTETIIVNGLMR
EEECHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECHHH
ARPGSTITPQMTELPRERQDTPPEAANAESGQ
CCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]