Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 209550205

GI number: 209550205

Start: 2669505

End: 2671172

Strand: Direct

Name: ilvB [H]

Synonym: Rleg2_2625

Alternate gene names: 209550205

Gene position: 2669505-2671172 (Clockwise)

Preceding gene: 209550204

Following gene: 209550209

Centisome position: 58.83

GC content: 64.51

Gene sequence:

>1668_bases
ATGAAAAGAACGGGCGGGCAACTCATCGTCGAGGCGCTGAAGGCGAACGGCGTCAAGCGTCTCTCCTGCGTGCCGGGCGA
GAGTTTCCTCGCCGTTCTCGACGCGCTGCGCGACAGCGATATCGATGTGATCGTCTGCCGCCAGGAGGGCGGCGCGGCGA
TGATGGCGGATTGCTGGGGCCGGCTGACCGGCGAACCCGGCATCTGCATGGTCACCCGCGGCCCCGGCGCCACCAACGCC
TCCGCCGGTCTGCATATCGCCAAGCAGGATTCGATCCCGATGATCCTCTTCATCGGCCAGGTGCAGCGCGAAGCCCGCGA
GCGCGAGGCCTTCCAGGAGGTCGAATTCCGCCGCGCCTTCACCGAATTCGCCAAATGGGTGGGTGAGATCGACGATGCCG
CCCGCATTCCCGAGTTCGTCACCCGCGCCTTCGCGATCGCAACCTCCGGCCGTCCCGGCCCCGTGGTGCTGACGCTGCCG
GAGGATATGCTGCGCGACGAGGTCGAAGCGCCCCGCGCCAAGCGTTATGTCAGCGTCGAGGCGCATCCCGGCCGCCGACA
GATCGACGATTTCTATATACGATTGCTCAAAGCCGAGCGGCCGATGGTGATCCTCGGCGGCACGCGCTGGGATGCCGATA
CCGTCGCCGATTTTGCAATCTTCGCCGAGCGTTTTCAATTGCCGGTCGGCTGCTCCTTCCGCCGGCAGATGCTGTTCGAT
CATCTCCATCCCTCTTATGCCGGCGATGTCGGCATCGGCATCAATCCGGCGCTGGCAAAGGAGATCAAGGAAAGCGACCT
GCTGATCCTGCTCGGCGCCCGCATGTCGGAAATGCCGTCTTCGTCCTATACGCTGATCGATATCCCCTACCCCCAGCAAT
CGCTGGTTCATGTCTATCCCGATGCGTCCGAACTCGGTCGCATCTACCGCCCGGATCTCGCCATCTGCGCCGCGCCTGCC
GATTTCGTCACCGCACTTGCCGAGCTGGAAGCCCCGGCCGAGCCGCATTGGGCGGAGCGCACTGAGCGCATGCACCAGGC
CTATCTCACCTGGTCGAAACCGCCGGCGACAGGTCCGGGCGCCGTCCATATGGGGCCGATCATGGAGTGGCTGGAAGCCA
ATACCGGGCCTGAGACGATCTTCACCAACGGCGCCGGCAACTACGCCACCTGGCTGCACCGCTTCCATCGTTTCCGCCGC
TTCAACACCCAGGCCGCGCCCACCTCAGGCTCGATGGGCTACGGCCTGCCGGCCGCCGTCGCCGCCAAGCGGCTCTTCCC
GGAGCGCGAGGTCATCTGCTTTGCCGGCGACGGCTGTTTCCTGATGCACGGCCAGGAATTCGCCACTGCCATCCGCTACG
GCCTGCCGATCATATCGGTCGTCGTCAACAACGGCATCTACGGCACGATCCGCATGCATCAGGAGCGCGAATATCCCGGC
CGCGTCAGCAGCACCGATCTCACCAATCCCGACTTCGCAGCCCTTGCCCGCGCTTATGGCGGCCATGGCGAGACGGTGGA
ACAGACGGAAGAATTCGCGCCCGCCTTCGAGCGGGCCCGCGCAAGCGGCAAGCCGGCAATCATTGAAGTCAAACTCGATC
CCGAGGCAATCACGCCGACACGCACGCTCTCGGAAATCGCGCAAACAAAAAAGCCGGTAAGTCCGTGA

Upstream 100 bases:

>100_bases
TACAGCAGGCCGAGCCCGCGTCTCGGCGAACACACCGAAGAAATTCTGGCGGAACTCGCCGAGCGCGAGAGGAAGGCATC
GTCATGAAGAAGGTATTGTC

Downstream 100 bases:

>100_bases
TCTCACGCACCTACCGGCTTTGACGCAATGCTGCGGCGACGATCAGTCGATCGCGGCCGTGACGATGAACTCGACCTTGT
ATTTCGGCGCTGCGAGCTTG

Product: thiamine pyrophosphatase

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKRTGGQLIVEALKANGVKRLSCVPGESFLAVLDALRDSDIDVIVCRQEGGAAMMADCWGRLTGEPGICMVTRGPGATNA
SAGLHIAKQDSIPMILFIGQVQREAREREAFQEVEFRRAFTEFAKWVGEIDDAARIPEFVTRAFAIATSGRPGPVVLTLP
EDMLRDEVEAPRAKRYVSVEAHPGRRQIDDFYIRLLKAERPMVILGGTRWDADTVADFAIFAERFQLPVGCSFRRQMLFD
HLHPSYAGDVGIGINPALAKEIKESDLLILLGARMSEMPSSSYTLIDIPYPQQSLVHVYPDASELGRIYRPDLAICAAPA
DFVTALAELEAPAEPHWAERTERMHQAYLTWSKPPATGPGAVHMGPIMEWLEANTGPETIFTNGAGNYATWLHRFHRFRR
FNTQAAPTSGSMGYGLPAAVAAKRLFPEREVICFAGDGCFLMHGQEFATAIRYGLPIISVVVNNGIYGTIRMHQEREYPG
RVSSTDLTNPDFAALARAYGGHGETVEQTEEFAPAFERARASGKPAIIEVKLDPEAITPTRTLSEIAQTKKPVSP

Sequences:

>Translated_555_residues
MKRTGGQLIVEALKANGVKRLSCVPGESFLAVLDALRDSDIDVIVCRQEGGAAMMADCWGRLTGEPGICMVTRGPGATNA
SAGLHIAKQDSIPMILFIGQVQREAREREAFQEVEFRRAFTEFAKWVGEIDDAARIPEFVTRAFAIATSGRPGPVVLTLP
EDMLRDEVEAPRAKRYVSVEAHPGRRQIDDFYIRLLKAERPMVILGGTRWDADTVADFAIFAERFQLPVGCSFRRQMLFD
HLHPSYAGDVGIGINPALAKEIKESDLLILLGARMSEMPSSSYTLIDIPYPQQSLVHVYPDASELGRIYRPDLAICAAPA
DFVTALAELEAPAEPHWAERTERMHQAYLTWSKPPATGPGAVHMGPIMEWLEANTGPETIFTNGAGNYATWLHRFHRFRR
FNTQAAPTSGSMGYGLPAAVAAKRLFPEREVICFAGDGCFLMHGQEFATAIRYGLPIISVVVNNGIYGTIRMHQEREYPG
RVSSTDLTNPDFAALARAYGGHGETVEQTEEFAPAFERARASGKPAIIEVKLDPEAITPTRTLSEIAQTKKPVSP
>Mature_555_residues
MKRTGGQLIVEALKANGVKRLSCVPGESFLAVLDALRDSDIDVIVCRQEGGAAMMADCWGRLTGEPGICMVTRGPGATNA
SAGLHIAKQDSIPMILFIGQVQREAREREAFQEVEFRRAFTEFAKWVGEIDDAARIPEFVTRAFAIATSGRPGPVVLTLP
EDMLRDEVEAPRAKRYVSVEAHPGRRQIDDFYIRLLKAERPMVILGGTRWDADTVADFAIFAERFQLPVGCSFRRQMLFD
HLHPSYAGDVGIGINPALAKEIKESDLLILLGARMSEMPSSSYTLIDIPYPQQSLVHVYPDASELGRIYRPDLAICAAPA
DFVTALAELEAPAEPHWAERTERMHQAYLTWSKPPATGPGAVHMGPIMEWLEANTGPETIFTNGAGNYATWLHRFHRFRR
FNTQAAPTSGSMGYGLPAAVAAKRLFPEREVICFAGDGCFLMHGQEFATAIRYGLPIISVVVNNGIYGTIRMHQEREYPG
RVSSTDLTNPDFAALARAYGGHGETVEQTEEFAPAFERARASGKPAIIEVKLDPEAITPTRTLSEIAQTKKPVSP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=25.1327433628319, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI21361361, Length=559, Percent_Identity=24.3291592128801, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=29.5698924731183, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1790104, Length=542, Percent_Identity=31.1808118081181, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=27.336860670194, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=24.4165170556553, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=26.056338028169, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=25.3075571177504, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=25.3075571177504, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=562, Percent_Identity=22.9537366548043, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=28.4191829484902, Blast_Score=207, Evalue=4e-54,
Organism=Drosophila melanogaster, GI19922626, Length=579, Percent_Identity=25.0431778929188, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61056; Mature: 61056

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTGGQLIVEALKANGVKRLSCVPGESFLAVLDALRDSDIDVIVCRQEGGAAMMADCWG
CCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
RLTGEPGICMVTRGPGATNASAGLHIAKQDSIPMILFIGQVQREAREREAFQEVEFRRAF
HCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
TEFAKWVGEIDDAARIPEFVTRAFAIATSGRPGPVVLTLPEDMLRDEVEAPRAKRYVSVE
HHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHHHHHHCCCCHHEEEEEE
AHPGRRQIDDFYIRLLKAERPMVILGGTRWDADTVADFAIFAERFQLPVGCSFRRQMLFD
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HLHPSYAGDVGIGINPALAKEIKESDLLILLGARMSEMPSSSYTLIDIPYPQQSLVHVYP
HCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHCCCCCCEEEEECCCCCCCEEEECC
DASELGRIYRPDLAICAAPADFVTALAELEAPAEPHWAERTERMHQAYLTWSKPPATGPG
CHHHHCHHCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
AVHMGPIMEWLEANTGPETIFTNGAGNYATWLHRFHRFRRFNTQAAPTSGSMGYGLPAAV
CEEHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AAKRLFPEREVICFAGDGCFLMHGQEFATAIRYGLPIISVVVNNGIYGTIRMHQEREYPG
HHHHHCCCCCEEEEECCCEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCC
RVSSTDLTNPDFAALARAYGGHGETVEQTEEFAPAFERARASGKPAIIEVKLDPEAITPT
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCH
RTLSEIAQTKKPVSP
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRTGGQLIVEALKANGVKRLSCVPGESFLAVLDALRDSDIDVIVCRQEGGAAMMADCWG
CCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
RLTGEPGICMVTRGPGATNASAGLHIAKQDSIPMILFIGQVQREAREREAFQEVEFRRAF
HCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
TEFAKWVGEIDDAARIPEFVTRAFAIATSGRPGPVVLTLPEDMLRDEVEAPRAKRYVSVE
HHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHHHHHHCCCCHHEEEEEE
AHPGRRQIDDFYIRLLKAERPMVILGGTRWDADTVADFAIFAERFQLPVGCSFRRQMLFD
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HLHPSYAGDVGIGINPALAKEIKESDLLILLGARMSEMPSSSYTLIDIPYPQQSLVHVYP
HCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHCCCCCCEEEEECCCCCCCEEEECC
DASELGRIYRPDLAICAAPADFVTALAELEAPAEPHWAERTERMHQAYLTWSKPPATGPG
CHHHHCHHCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
AVHMGPIMEWLEANTGPETIFTNGAGNYATWLHRFHRFRRFNTQAAPTSGSMGYGLPAAV
CEEHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AAKRLFPEREVICFAGDGCFLMHGQEFATAIRYGLPIISVVVNNGIYGTIRMHQEREYPG
HHHHHCCCCCEEEEECCCEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCC
RVSSTDLTNPDFAALARAYGGHGETVEQTEEFAPAFERARASGKPAIIEVKLDPEAITPT
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCH
RTLSEIAQTKKPVSP
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]