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The map label for this gene is pncA [H]

Identifier: 209550147

GI number: 209550147

Start: 2605281

End: 2605907

Strand: Direct

Name: pncA [H]

Synonym: Rleg2_2566

Alternate gene names: 209550147

Gene position: 2605281-2605907 (Clockwise)

Preceding gene: 209550146

Following gene: 209550150

Centisome position: 57.41

GC content: 63.16

Gene sequence:

>627_bases
ATGAAAGCGCTGCTGCTCGTCGACATTCAGAACGGCTTCTGTCCGGGCGGCAATCTGCCGGTCCCGGATGGCGATAAAGT
GGTTCCTATCGCAAACAGCCTGATCGACAGCGGCAAATACGATCTGATCGTCGCCTCCCAGGATTGGCACCCGCCGGGCC
ATGGCAGTTTCGCCTCCGCCCATCCGGGTGCGGCACCTTTCGAGATGGGCGAACTGTCGGGCAAGCCGCAGATGATGTGG
CCCGACCACTGCATTCAGGGCACGCTCGATGCCGAACTGCATCCCGCGCTGAAATCCGAAGAGATCGACCTGATCCAGCA
GAAGGGCGAGGATCCCGACATCGACAGCTATTCCGCCTTCCGCGACAATGACCGCGATGCCTCGACCGGCCTTGCCGAAT
TCCTCGAGGGCCAGAGTGTCACCGATCTCGACGTCTGCGGGCTGGCGACCGACTATTGCGTCAAGTTCTCCGCGCTCGAC
GCGCTGGAAATGATCCCCGGTGTCCGCGTCCGCTTCATCGAGGATGCAAGCCGCGGCATCACCCCCGAAGGCGTCGCCGC
CGCCATCGACGAGATGCGCGCGCATGGCATCGCGATCATCGGCAGCGCTAAGGTGCTGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCAGATTTAACACTTGCGCGGCGGCGCCTGGAAATCCACTAGTTGATGCGTTCAATATGCTTGGCGTCTGACACTGGAAT
AAGCGAAGGGATTGGCCTTG

Downstream 100 bases:

>100_bases
TTCACGCCAAAGACAATGTGGGGAATGTCGGATTGGCGCACGCCCATCCGTCCATGGCTCTGAACAACGAGAGGGGCTGC
TGTCCGGCGTCCTATTTCAG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKALLLVDIQNGFCPGGNLPVPDGDKVVPIANSLIDSGKYDLIVASQDWHPPGHGSFASAHPGAAPFEMGELSGKPQMMW
PDHCIQGTLDAELHPALKSEEIDLIQQKGEDPDIDSYSAFRDNDRDASTGLAEFLEGQSVTDLDVCGLATDYCVKFSALD
ALEMIPGVRVRFIEDASRGITPEGVAAAIDEMRAHGIAIIGSAKVLAG

Sequences:

>Translated_208_residues
MKALLLVDIQNGFCPGGNLPVPDGDKVVPIANSLIDSGKYDLIVASQDWHPPGHGSFASAHPGAAPFEMGELSGKPQMMW
PDHCIQGTLDAELHPALKSEEIDLIQQKGEDPDIDSYSAFRDNDRDASTGLAEFLEGQSVTDLDVCGLATDYCVKFSALD
ALEMIPGVRVRFIEDASRGITPEGVAAAIDEMRAHGIAIIGSAKVLAG
>Mature_208_residues
MKALLLVDIQNGFCPGGNLPVPDGDKVVPIANSLIDSGKYDLIVASQDWHPPGHGSFASAHPGAAPFEMGELSGKPQMMW
PDHCIQGTLDAELHPALKSEEIDLIQQKGEDPDIDSYSAFRDNDRDASTGLAEFLEGQSVTDLDVCGLATDYCVKFSALD
ALEMIPGVRVRFIEDASRGITPEGVAAAIDEMRAHGIAIIGSAKVLAG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=202, Percent_Identity=50.4950495049505, Blast_Score=194, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI193206735, Length=222, Percent_Identity=32.8828828828829, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17543130, Length=222, Percent_Identity=32.8828828828829, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71999339, Length=221, Percent_Identity=30.316742081448, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321401, Length=216, Percent_Identity=31.4814814814815, Blast_Score=113, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648247, Length=229, Percent_Identity=32.7510917030568, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22056; Mature: 22056

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLLVDIQNGFCPGGNLPVPDGDKVVPIANSLIDSGKYDLIVASQDWHPPGHGSFASA
CCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
HPGAAPFEMGELSGKPQMMWPDHCIQGTLDAELHPALKSEEIDLIQQKGEDPDIDSYSAF
CCCCCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHH
RDNDRDASTGLAEFLEGQSVTDLDVCGLATDYCVKFSALDALEMIPGVRVRFIEDASRGI
CCCCCCHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCC
TPEGVAAAIDEMRAHGIAIIGSAKVLAG
CCHHHHHHHHHHHHCCEEEEECCEECCC
>Mature Secondary Structure
MKALLLVDIQNGFCPGGNLPVPDGDKVVPIANSLIDSGKYDLIVASQDWHPPGHGSFASA
CCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
HPGAAPFEMGELSGKPQMMWPDHCIQGTLDAELHPALKSEEIDLIQQKGEDPDIDSYSAF
CCCCCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHH
RDNDRDASTGLAEFLEGQSVTDLDVCGLATDYCVKFSALDALEMIPGVRVRFIEDASRGI
CCCCCCHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCC
TPEGVAAAIDEMRAHGIAIIGSAKVLAG
CCHHHHHHHHHHHHCCEEEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]