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The map label for this gene is yafC [H]

Identifier: 209550132

GI number: 209550132

Start: 2583842

End: 2584744

Strand: Direct

Name: yafC [H]

Synonym: Rleg2_2550

Alternate gene names: 209550132

Gene position: 2583842-2584744 (Clockwise)

Preceding gene: 209550128

Following gene: 209550133

Centisome position: 56.94

GC content: 67.0

Gene sequence:

>903_bases
ATGAGCCGTCAGGACATCAACCGCTCCGGCGAAATGGAGGTCTTCGTCAGCGTCGTCGAGCGCGGCGGCTTTACCGCGGC
CGCCCTCGCCCGGCGCATGACGCCGTCGGCCGTCAGCAAGCTCGTCGCTAGGCTGGAGCAACGCCTCGGCGCCCGCCTTG
TCAATCGATCGACGCGAAAACTGCAGCTGACGCCGGAGGGTTGCGCCTTCTACGAGCGCAGCATCGCCATCCTGGCCGAT
ATTGCCGAGGCCGAGCGCCAGGCCTCATCAGGCGAACAGGCTTCGGGCCGCATCCGTATCAACACCAGCGGCTCTTTCGG
CAATCATGTGCTGGCGCCGCTGATGCCGGCCTTCATGGCGCTTCATCCCGCCGTGACGCTGGACATTTCCCATACCGACA
GGATCGTCGACCTGATGGAGGAGCGCGCCGATGTCGCGATCCGCGCCGGCCCCTTGAAGAATTCCAGCCTGATCGCCCGC
AAGCTCGGCGCCACCGGCAAGATCATCGTCGCCTCGCCGGATTATCTCAGGCGTCACGGCGAACCGCGCACGGTCGCCGA
TCTCCGCCGCCATTGCCGCATCGGCTTTTCCTATGCCCGCGCCGTCGAGGGCTGGCCGCTGCGCGAGGACGGCGAAACGA
TAACCATCCCGATCACCCCCGGCGTCCAGGTGGGAGACGGCGAAGCCATGCGCCACCTCGCCTTGTCAGGCGCAGGCCTT
GCCCGCCTTGCTGCCTTCACCGTGCGCGCCGACATCGATGCCGGACGCTTGATGCCGGTGCTCGATGAGGCGAATCCCGG
CGATCTCGAAGAATTCTACGCCCTCTACATGGGCCAGGGCGGCCCGCTGCCGGCGCGCGTGCGCGCCCTGCTCGACTTTC
TCGCCAGCCATGTGCGCCTCTGA

Upstream 100 bases:

>100_bases
TAGTCCATGGATCTGTCTCCTTCGTTTCCACTAAGATAGACCTCGACCGCGCGGTGATATAGATTGCCTGCGAGCAAATC
ATCTGTGACTTGAATTCATC

Downstream 100 bases:

>100_bases
TAAATTCCGCCCAAACCGTTTGGCGACTTGACGCGATGACATGGAAAAACAATCGCTTGAAGCGGCCAGATGAATCAGTT
TGGCATCCGCGAGCGGTGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSRQDINRSGEMEVFVSVVERGGFTAAALARRMTPSAVSKLVARLEQRLGARLVNRSTRKLQLTPEGCAFYERSIAILAD
IAEAERQASSGEQASGRIRINTSGSFGNHVLAPLMPAFMALHPAVTLDISHTDRIVDLMEERADVAIRAGPLKNSSLIAR
KLGATGKIIVASPDYLRRHGEPRTVADLRRHCRIGFSYARAVEGWPLREDGETITIPITPGVQVGDGEAMRHLALSGAGL
ARLAAFTVRADIDAGRLMPVLDEANPGDLEEFYALYMGQGGPLPARVRALLDFLASHVRL

Sequences:

>Translated_300_residues
MSRQDINRSGEMEVFVSVVERGGFTAAALARRMTPSAVSKLVARLEQRLGARLVNRSTRKLQLTPEGCAFYERSIAILAD
IAEAERQASSGEQASGRIRINTSGSFGNHVLAPLMPAFMALHPAVTLDISHTDRIVDLMEERADVAIRAGPLKNSSLIAR
KLGATGKIIVASPDYLRRHGEPRTVADLRRHCRIGFSYARAVEGWPLREDGETITIPITPGVQVGDGEAMRHLALSGAGL
ARLAAFTVRADIDAGRLMPVLDEANPGDLEEFYALYMGQGGPLPARVRALLDFLASHVRL
>Mature_299_residues
SRQDINRSGEMEVFVSVVERGGFTAAALARRMTPSAVSKLVARLEQRLGARLVNRSTRKLQLTPEGCAFYERSIAILADI
AEAERQASSGEQASGRIRINTSGSFGNHVLAPLMPAFMALHPAVTLDISHTDRIVDLMEERADVAIRAGPLKNSSLIARK
LGATGKIIVASPDYLRRHGEPRTVADLRRHCRIGFSYARAVEGWPLREDGETITIPITPGVQVGDGEAMRHLALSGAGLA
RLAAFTVRADIDAGRLMPVLDEANPGDLEEFYALYMGQGGPLPARVRALLDFLASHVRL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=38.0952380952381, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=31.9587628865979, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693193, Length=307, Percent_Identity=30.9446254071661, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.6928327645051, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=27.6450511945392, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=26.8456375838926, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=30.3571428571429, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=27.9527559055118, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788481, Length=264, Percent_Identity=24.6212121212121, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=23.3108108108108, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=28.7581699346405, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32528; Mature: 32397

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQDINRSGEMEVFVSVVERGGFTAAALARRMTPSAVSKLVARLEQRLGARLVNRSTRK
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCE
LQLTPEGCAFYERSIAILADIAEAERQASSGEQASGRIRINTSGSFGNHVLAPLMPAFMA
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
LHPAVTLDISHTDRIVDLMEERADVAIRAGPLKNSSLIARKLGATGKIIVASPDYLRRHG
HCCEEEEEECCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCCEEEEECCHHHHHCC
EPRTVADLRRHCRIGFSYARAVEGWPLREDGETITIPITPGVQVGDGEAMRHLALSGAGL
CCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCCEECCCHHHHHHHHCCCCH
ARLAAFTVRADIDAGRLMPVLDEANPGDLEEFYALYMGQGGPLPARVRALLDFLASHVRL
HHHHHHHEEECCCCCCCEEHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRQDINRSGEMEVFVSVVERGGFTAAALARRMTPSAVSKLVARLEQRLGARLVNRSTRK
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCE
LQLTPEGCAFYERSIAILADIAEAERQASSGEQASGRIRINTSGSFGNHVLAPLMPAFMA
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
LHPAVTLDISHTDRIVDLMEERADVAIRAGPLKNSSLIARKLGATGKIIVASPDYLRRHG
HCCEEEEEECCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCCEEEEECCHHHHHCC
EPRTVADLRRHCRIGFSYARAVEGWPLREDGETITIPITPGVQVGDGEAMRHLALSGAGL
CCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCCEECCCHHHHHHHHCCCCH
ARLAAFTVRADIDAGRLMPVLDEANPGDLEEFYALYMGQGGPLPARVRALLDFLASHVRL
HHHHHHHEEECCCCCCCEEHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]