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The map label for this gene is yafC [C]

Identifier: 209549940

GI number: 209549940

Start: 2417465

End: 2418388

Strand: Direct

Name: yafC [C]

Synonym: Rleg2_2356

Alternate gene names: 209549940

Gene position: 2417465-2418388 (Clockwise)

Preceding gene: 209549939

Following gene: 209549944

Centisome position: 53.27

GC content: 65.48

Gene sequence:

>924_bases
ATGCCGCGCACGAATCTGAACGATATCCTGATCTTCATGGCCGTCGTCGATGCCGGAAGTTTTATCGCCGGCGGCCAGGC
CATGGGCCTGTCCCGCTCGGCGGCTGGAAAGGCCGTCATCCGCCTCGAAGACCGGCTCGGCGCACGTCTGCTCAACCGCA
CGACGAGAACGCTGAGCCTGACCGATGAGGGGCGGGTATTTTACGAGCGCGGCCTGCAGATCCTTGCGTCGGTCGCCGAG
GCGGAAGCGAGTCTGGGAGGCCAGAGCGGCATGCCGCGCGGCCTTCTCAGACTGACTGTACCCGATGCCTTCGGCCGGCT
CGTCGTGCTGCCCCTGCTGGAAAAATATCTTCGGGCCTGGCCCGACATCCAGGTCGAGGTGAGCTTTACCGATCACCTCT
CCGACATCATCGAGGACGGCTTCGATCTGGCGATCCGTATCGGCGCCGCGGCGTCGGATACCCGGCTGGTCTCGCGCGTG
ATCGCCACCTACAAGACACGCCTCTGCGCCTCGCCGTCCTATCTCGCCGCGAACGGCGCGCCGCGCGAAATCGAGGATCT
CGCAGCCCACGACTGCCTGATTTTTGCCAACCGCAATCAAAGGCAAGGCTGGCGTTTTCGCGGCAAGGGCGGTTCCTGGA
TCAAGGCGCAGGGGCGCAGCCGCCTGAGGCTCGACAGCGGCGAGGCGATCCGCGACGCTGCCCTGGCGGGATTGGGCATC
GCGCTCCTGCCCGATTTTCTCATCGCCGGCGATCTCGCCGCCGGGCGTCTCCGGCAGGTCCTGCCCGACCTGGAAGCCGA
TGACGCCAAGATCGTCACGCTCTATCCCGACAAGCGCCTGCTGGAACCGCGCATCCACCGTTTCGTCGATCTGATGGTCG
AGGCGTTGGGGCATGAGAGATCGGACGCGCGCTCATCGCCTTGA

Upstream 100 bases:

>100_bases
TCGGGACCTTACTGCCGCCGTGAGCGTTGATGGTCCAAGTCTTGAATCGGTTGCATGACTGACCGACTTATGGCTGGGAC
CGAACCGCTCGAGTTGCCTG

Downstream 100 bases:

>100_bases
TACGGAAGCACCGGCATCGGCAATTGCCCTGTCGAAACCGCGGAAACTTTTCAGGGCAAGGGGAAAGAGAGTGGCGACGA
GATAGGCAAGCCCGGTGAGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPRTNLNDILIFMAVVDAGSFIAGGQAMGLSRSAAGKAVIRLEDRLGARLLNRTTRTLSLTDEGRVFYERGLQILASVAE
AEASLGGQSGMPRGLLRLTVPDAFGRLVVLPLLEKYLRAWPDIQVEVSFTDHLSDIIEDGFDLAIRIGAAASDTRLVSRV
IATYKTRLCASPSYLAANGAPREIEDLAAHDCLIFANRNQRQGWRFRGKGGSWIKAQGRSRLRLDSGEAIRDAALAGLGI
ALLPDFLIAGDLAAGRLRQVLPDLEADDAKIVTLYPDKRLLEPRIHRFVDLMVEALGHERSDARSSP

Sequences:

>Translated_307_residues
MPRTNLNDILIFMAVVDAGSFIAGGQAMGLSRSAAGKAVIRLEDRLGARLLNRTTRTLSLTDEGRVFYERGLQILASVAE
AEASLGGQSGMPRGLLRLTVPDAFGRLVVLPLLEKYLRAWPDIQVEVSFTDHLSDIIEDGFDLAIRIGAAASDTRLVSRV
IATYKTRLCASPSYLAANGAPREIEDLAAHDCLIFANRNQRQGWRFRGKGGSWIKAQGRSRLRLDSGEAIRDAALAGLGI
ALLPDFLIAGDLAAGRLRQVLPDLEADDAKIVTLYPDKRLLEPRIHRFVDLMVEALGHERSDARSSP
>Mature_306_residues
PRTNLNDILIFMAVVDAGSFIAGGQAMGLSRSAAGKAVIRLEDRLGARLLNRTTRTLSLTDEGRVFYERGLQILASVAEA
EASLGGQSGMPRGLLRLTVPDAFGRLVVLPLLEKYLRAWPDIQVEVSFTDHLSDIIEDGFDLAIRIGAAASDTRLVSRVI
ATYKTRLCASPSYLAANGAPREIEDLAAHDCLIFANRNQRQGWRFRGKGGSWIKAQGRSRLRLDSGEAIRDAALAGLGIA
LLPDFLIAGDLAAGRLRQVLPDLEADDAKIVTLYPDKRLLEPRIHRFVDLMVEALGHERSDARSSP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=31.8339100346021, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=36.5384615384615, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=30.9278350515464, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789440, Length=269, Percent_Identity=30.4832713754647, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=30.6896551724138, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1789639, Length=285, Percent_Identity=28.4210526315789, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=26.027397260274, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788481, Length=297, Percent_Identity=24.5791245791246, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=28.3333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33467; Mature: 33336

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTNLNDILIFMAVVDAGSFIAGGQAMGLSRSAAGKAVIRLEDRLGARLLNRTTRTLSL
CCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCEEEEE
TDEGRVFYERGLQILASVAEAEASLGGQSGMPRGLLRLTVPDAFGRLVVLPLLEKYLRAW
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
PDIQVEVSFTDHLSDIIEDGFDLAIRIGAAASDTRLVSRVIATYKTRLCASPSYLAANGA
CCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCC
PREIEDLAAHDCLIFANRNQRQGWRFRGKGGSWIKAQGRSRLRLDSGEAIRDAALAGLGI
CHHHHHHHHCCEEEEECCCCCCCCEEECCCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
ALLPDFLIAGDLAAGRLRQVLPDLEADDAKIVTLYPDKRLLEPRIHRFVDLMVEALGHER
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
SDARSSP
CCCCCCC
>Mature Secondary Structure 
PRTNLNDILIFMAVVDAGSFIAGGQAMGLSRSAAGKAVIRLEDRLGARLLNRTTRTLSL
CCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCEEEEE
TDEGRVFYERGLQILASVAEAEASLGGQSGMPRGLLRLTVPDAFGRLVVLPLLEKYLRAW
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
PDIQVEVSFTDHLSDIIEDGFDLAIRIGAAASDTRLVSRVIATYKTRLCASPSYLAANGA
CCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCC
PREIEDLAAHDCLIFANRNQRQGWRFRGKGGSWIKAQGRSRLRLDSGEAIRDAALAGLGI
CHHHHHHHHCCEEEEECCCCCCCCEEECCCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
ALLPDFLIAGDLAAGRLRQVLPDLEADDAKIVTLYPDKRLLEPRIHRFVDLMVEALGHER
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
SDARSSP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]