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The map label for this gene is opdE [H]

Identifier: 209549903

GI number: 209549903

Start: 2376872

End: 2378062

Strand: Direct

Name: opdE [H]

Synonym: Rleg2_2319

Alternate gene names: 209549903

Gene position: 2376872-2378062 (Clockwise)

Preceding gene: 209549902

Following gene: 209549904

Centisome position: 52.38

GC content: 64.32

Gene sequence:

>1191_bases
ATGGACATCAACTACGACATGAGAATTGAAGCCAAGACGACGCCGTGGAGTGCGGTCATCTGCATGATGCTGACCTCCTT
CGTGCTCGTGGCGTCGGAATTCATGCCGGTGAGCCTGCTGACCCCGATCGCCGACGAACTGGCGAGCACCGCAGGCCAGG
CGGGTCAGGCGATCTCGATTTCGGGTTTCTTCGCCGTCATCACCGCCTTGTTCAGCAATGTGCTGTTTGCGCGCTTCGAC
CGGCGCCGGGTGATCCTCTGCTACACCGTCGTGCTGGTGGCGTCCGGCCTTGCGGTCACCTTTGCCCCCAACTACCTTAT
CTTCATGGTCGGCCGCGCGCTCATCGGTGTGTCGATCGGCGGCTACTGGTCCTTGGCAACGGCGATCATCGCCCGCATTG
CCTCCGGTCCCGACGTGCCCAAGGCGCTGGCCATGCTTCAAGGCGGCAGCGCGCTCGCCGCCGTGATCGCCGCCCCGCTT
GGAAGTTTCCTCGGCAGCCTTGTCGGATGGCGCGGCGCCTTCTTCATCGTCGTGCCGATCGGCATTGTCGCCTTCATCTG
GCAAGCCATCGCCCTGCCCCGGATGCCGGGCGGCCAAAGCGGATCGCTCGGCAGGACGTTCCGCCTGATGGGCAACCGCA
CCTTCGCCCTTGGCATGACGGCGATGATCCTGTTTTTCATGGGGCAATTTGCGCTCTCGACCTATCTGAGGCCGTTTCTC
GAAGACATCACCCATCTCGGCGTCAACGCGCTCTCGCTGGTGCTGCTCGGGATCGGCCTTGCCGGCCTCGCCGGAACCTC
GCTGATCCCCTCCATGCTGCGCGCGCATATGGCCCACGTGCTGATCGGGTTTCCGGCGGTGCTGGTGACCGTGGCCTTGG
CGCTCGTCGGCCTCGGCCCCGTGGCCTTCGCGACGGCCGGCCTGCTGCTTTTCTGGGGCTTGCTGACGACGCCGGTGCCG
GCGGCATGGACGACCTGGATGACGCGGACGGTCCCGCACCATCTGGAGGAAGCCGGCGCCTGGTTTGTCGCGCTTATTCA
GTTTGCGATCACTTCAGGGGCATTCGCCGGCGGTCTGTTGTTCGATCATATCGGCTGGTGGAGCCCGTTCGTATTGAGTG
CGGTGACTATGCTGGGTTCGGCGGTGACTGCGGTTGGCGTGACGCGGGCATCCAAGAGAGCCTCATCCTGA

Upstream 100 bases:

>100_bases
GACGCCGGCATGGCTTGCCGGCGCGACCCTGGTCGCCGGAACGGCGGCGCGCAGCCGCCGCCCGGCGCCATGAACACTCC
CGCTTTGAAGAGAAAGCCCG

Downstream 100 bases:

>100_bases
GGTGCCCCGCAGGGCCTCGAAGGACGAGGCGGGCGCTCGACGTCAGATGGGTGCAAGAAGCAGCGTTGCGGCGTGCTTCG
AGGCTCCGCCCTCTCGGGCT

Product: major facilitator superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MDINYDMRIEAKTTPWSAVICMMLTSFVLVASEFMPVSLLTPIADELASTAGQAGQAISISGFFAVITALFSNVLFARFD
RRRVILCYTVVLVASGLAVTFAPNYLIFMVGRALIGVSIGGYWSLATAIIARIASGPDVPKALAMLQGGSALAAVIAAPL
GSFLGSLVGWRGAFFIVVPIGIVAFIWQAIALPRMPGGQSGSLGRTFRLMGNRTFALGMTAMILFFMGQFALSTYLRPFL
EDITHLGVNALSLVLLGIGLAGLAGTSLIPSMLRAHMAHVLIGFPAVLVTVALALVGLGPVAFATAGLLLFWGLLTTPVP
AAWTTWMTRTVPHHLEEAGAWFVALIQFAITSGAFAGGLLFDHIGWWSPFVLSAVTMLGSAVTAVGVTRASKRASS

Sequences:

>Translated_396_residues
MDINYDMRIEAKTTPWSAVICMMLTSFVLVASEFMPVSLLTPIADELASTAGQAGQAISISGFFAVITALFSNVLFARFD
RRRVILCYTVVLVASGLAVTFAPNYLIFMVGRALIGVSIGGYWSLATAIIARIASGPDVPKALAMLQGGSALAAVIAAPL
GSFLGSLVGWRGAFFIVVPIGIVAFIWQAIALPRMPGGQSGSLGRTFRLMGNRTFALGMTAMILFFMGQFALSTYLRPFL
EDITHLGVNALSLVLLGIGLAGLAGTSLIPSMLRAHMAHVLIGFPAVLVTVALALVGLGPVAFATAGLLLFWGLLTTPVP
AAWTTWMTRTVPHHLEEAGAWFVALIQFAITSGAFAGGLLFDHIGWWSPFVLSAVTMLGSAVTAVGVTRASKRASS
>Mature_396_residues
MDINYDMRIEAKTTPWSAVICMMLTSFVLVASEFMPVSLLTPIADELASTAGQAGQAISISGFFAVITALFSNVLFARFD
RRRVILCYTVVLVASGLAVTFAPNYLIFMVGRALIGVSIGGYWSLATAIIARIASGPDVPKALAMLQGGSALAAVIAAPL
GSFLGSLVGWRGAFFIVVPIGIVAFIWQAIALPRMPGGQSGSLGRTFRLMGNRTFALGMTAMILFFMGQFALSTYLRPFL
EDITHLGVNALSLVLLGIGLAGLAGTSLIPSMLRAHMAHVLIGFPAVLVTVALALVGLGPVAFATAGLLLFWGLLTTPVP
AAWTTWMTRTVPHHLEEAGAWFVALIQFAITSGAFAGGLLFDHIGWWSPFVLSAVTMLGSAVTAVGVTRASKRASS

Specific function: Regulates the expression of oprD which encodes the imipenem-specific porin [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ywfA [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=388, Percent_Identity=39.4329896907216, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1787808, Length=240, Percent_Identity=23.75, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787947, Length=316, Percent_Identity=23.7341772151899, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786595, Length=250, Percent_Identity=22, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41658; Mature: 41658

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINYDMRIEAKTTPWSAVICMMLTSFVLVASEFMPVSLLTPIADELASTAGQAGQAISI
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEH
SGFFAVITALFSNVLFARFDRRRVILCYTVVLVASGLAVTFAPNYLIFMVGRALIGVSIG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCC
GYWSLATAIIARIASGPDVPKALAMLQGGSALAAVIAAPLGSFLGSLVGWRGAFFIVVPI
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GIVAFIWQAIALPRMPGGQSGSLGRTFRLMGNRTFALGMTAMILFFMGQFALSTYLRPFL
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDITHLGVNALSLVLLGIGLAGLAGTSLIPSMLRAHMAHVLIGFPAVLVTVALALVGLGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VAFATAGLLLFWGLLTTPVPAAWTTWMTRTVPHHLEEAGAWFVALIQFAITSGAFAGGLL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FDHIGWWSPFVLSAVTMLGSAVTAVGVTRASKRASS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDINYDMRIEAKTTPWSAVICMMLTSFVLVASEFMPVSLLTPIADELASTAGQAGQAISI
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEH
SGFFAVITALFSNVLFARFDRRRVILCYTVVLVASGLAVTFAPNYLIFMVGRALIGVSIG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCC
GYWSLATAIIARIASGPDVPKALAMLQGGSALAAVIAAPLGSFLGSLVGWRGAFFIVVPI
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GIVAFIWQAIALPRMPGGQSGSLGRTFRLMGNRTFALGMTAMILFFMGQFALSTYLRPFL
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDITHLGVNALSLVLLGIGLAGLAGTSLIPSMLRAHMAHVLIGFPAVLVTVALALVGLGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VAFATAGLLLFWGLLTTPVPAAWTTWMTRTVPHHLEEAGAWFVALIQFAITSGAFAGGLL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FDHIGWWSPFVLSAVTMLGSAVTAVGVTRASKRASS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1427017; 10984043 [H]