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The map label for this gene is metG [H]

Identifier: 209549396

GI number: 209549396

Start: 1849805

End: 1851355

Strand: Reverse

Name: metG [H]

Synonym: Rleg2_1802

Alternate gene names: 209549396

Gene position: 1851355-1849805 (Counterclockwise)

Preceding gene: 209549398

Following gene: 209549395

Centisome position: 40.8

GC content: 61.64

Gene sequence:

>1551_bases
ATGACAGACAAGACACCCTTCTACATCACCACCGCGATTTCCTATCCCAACGGCAAGCCGCATATCGGCCACGCCTATGA
GCTGATCGCGACGGATGCGATGGCGCGCTACCAGCGCCTGGATGGCAGGGATGTGTTCTTCCTGACCGGCACCGACGAAC
ACGGCCAGAAGATGCAGCAGACGGCGCGCGCCGAGGGGATTACCGCCCAGGCGCTTGCCGACCGCAACTCCGGCGAATTC
CAGGCGATGGCCAAGCTGCTCAACGCTTCGAACGATGATTTCATCCGCACCACGCAGGAGCGTCATCACGAAACATCGCG
CAATATCTGGAACCTGATGGCCGAGAATGGCGACATCTACAAGGATAGCTATGCCGGCTGGTATTCAGTGCGCGACGAGG
CCTATTACCAGGAAAACGAGACGGAGCTGCGCGCCGACGGCGTGCGCTACGGGCCGCAGGGCACACCTGTGGAATGGGTG
GAAGAGGCAAGCTATTTCTTCAAGCTCTCCGAATACCAGGAGAAGCTGCTTGCGCATTACGAGGCGAACCCCGATTTCAT
CGGCCCGGCCGAGCGCCGCAACGAGGTGATTTCCTTCGTCAAATCCGGCCTCAAGGATCTCTCGGTCTCGCGCACGACCT
TCGACTGGGGCATCAAGGTGCCGAACGATCCCGCCCATGTCATGTATGTCTGGGTCGACGCGCTGACCAACTACATCACC
GCGACCGGCTACATCGAGGACAGGAACGGCCCGCGGGCGAAATATTGGCCAGCCGATGTGCACATTATCGGCAAGGACAT
CATCCGCTTCCATGCCGTTTACTGGCCGGCCTTCCTGATGTCGGCAAAGCTGCCGCTGCCGAAGCGGGTCTTCGCCCATG
GCTTCCTGCTCAACAAGGGCGAGAAGATGTCGAAATCACTCGGCAACGTCGTCGATCCCGTCAATCTGGTCAGCCATTTC
GGCCTCGACCAGGTGCGCTACTTCTTCCTGCGCGAAGTCTCGTTCGGCCAGGACGGCAGCTACAGCGAAGAGGCGATCGG
TATCCGCATCAACTCCGACCTCGCCAACGGCATCGGCAACCTCGCCAGCCGCTCGCTGTCGATGATCGTCAAGAACTGCG
ACGGCAAGATCCCGGAATGCGGGGTGCTCAGCGATGAAGACAAGGCAATGCTGGCGCAGGCCGACGCGCTGCATGCCTCG
ACGCGCGACGACATGGGCAAGCAGCAGATCCACCGGGCGCTCGCCTCGATCATTTCGGTCGTCTCCGAGACCGACCGCTA
TTTCGCCGGCCAGGCGCCGTGGGCGCTGAAGAAAACCGATCCGGCGCGCATGGGCACGGTGCTTTACGTCACCGCCGAAG
TGGTACGCCAGATCGCCATCCTGCTGCAGCCCTTCATGCCGGATTCCTCGGGCAAGCTGCTCGATCTGGTCGCGGCACCT
GCAGACAAACGCGATTTTGCTGCTCTTGGCGAATCCGGCCGTCTCCTTGCCGGAACGCCGCTCGAAGCGCCGACGCCGGT
CTTCCCGCGCTACGTGGCTCCGGAGGCTTGA

Upstream 100 bases:

>100_bases
GGAAATCATTGGCGGCGGCAAAACTTGATGTTTCGCTTGACGGTGACATGCGCTAAGAGCGGCTGACCATTTTTATAGAG
TAAGCCGGACATTGGCCGCC

Downstream 100 bases:

>100_bases
GGCCGTGCTGATCGATACGCATTGCCATCTTGATTTCGCCGACTTCGAGGCGGAGCGCGACGAGATCGTTTCGCGCGCCC
ATCAGGCGGGCGTCAAGCAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTDKTPFYITTAISYPNGKPHIGHAYELIATDAMARYQRLDGRDVFFLTGTDEHGQKMQQTARAEGITAQALADRNSGEF
QAMAKLLNASNDDFIRTTQERHHETSRNIWNLMAENGDIYKDSYAGWYSVRDEAYYQENETELRADGVRYGPQGTPVEWV
EEASYFFKLSEYQEKLLAHYEANPDFIGPAERRNEVISFVKSGLKDLSVSRTTFDWGIKVPNDPAHVMYVWVDALTNYIT
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVFAHGFLLNKGEKMSKSLGNVVDPVNLVSHF
GLDQVRYFFLREVSFGQDGSYSEEAIGIRINSDLANGIGNLASRSLSMIVKNCDGKIPECGVLSDEDKAMLAQADALHAS
TRDDMGKQQIHRALASIISVVSETDRYFAGQAPWALKKTDPARMGTVLYVTAEVVRQIAILLQPFMPDSSGKLLDLVAAP
ADKRDFAALGESGRLLAGTPLEAPTPVFPRYVAPEA

Sequences:

>Translated_516_residues
MTDKTPFYITTAISYPNGKPHIGHAYELIATDAMARYQRLDGRDVFFLTGTDEHGQKMQQTARAEGITAQALADRNSGEF
QAMAKLLNASNDDFIRTTQERHHETSRNIWNLMAENGDIYKDSYAGWYSVRDEAYYQENETELRADGVRYGPQGTPVEWV
EEASYFFKLSEYQEKLLAHYEANPDFIGPAERRNEVISFVKSGLKDLSVSRTTFDWGIKVPNDPAHVMYVWVDALTNYIT
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVFAHGFLLNKGEKMSKSLGNVVDPVNLVSHF
GLDQVRYFFLREVSFGQDGSYSEEAIGIRINSDLANGIGNLASRSLSMIVKNCDGKIPECGVLSDEDKAMLAQADALHAS
TRDDMGKQQIHRALASIISVVSETDRYFAGQAPWALKKTDPARMGTVLYVTAEVVRQIAILLQPFMPDSSGKLLDLVAAP
ADKRDFAALGESGRLLAGTPLEAPTPVFPRYVAPEA
>Mature_515_residues
TDKTPFYITTAISYPNGKPHIGHAYELIATDAMARYQRLDGRDVFFLTGTDEHGQKMQQTARAEGITAQALADRNSGEFQ
AMAKLLNASNDDFIRTTQERHHETSRNIWNLMAENGDIYKDSYAGWYSVRDEAYYQENETELRADGVRYGPQGTPVEWVE
EASYFFKLSEYQEKLLAHYEANPDFIGPAERRNEVISFVKSGLKDLSVSRTTFDWGIKVPNDPAHVMYVWVDALTNYITA
TGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVFAHGFLLNKGEKMSKSLGNVVDPVNLVSHFG
LDQVRYFFLREVSFGQDGSYSEEAIGIRINSDLANGIGNLASRSLSMIVKNCDGKIPECGVLSDEDKAMLAQADALHAST
RDDMGKQQIHRALASIISVVSETDRYFAGQAPWALKKTDPARMGTVLYVTAEVVRQIAILLQPFMPDSSGKLLDLVAAPA
DKRDFAALGESGRLLAGTPLEAPTPVFPRYVAPEA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=540, Percent_Identity=36.1111111111111, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI14043022, Length=547, Percent_Identity=25.2285191956124, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788432, Length=499, Percent_Identity=27.0541082164329, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI212645096, Length=498, Percent_Identity=35.9437751004016, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI71992781, Length=387, Percent_Identity=35.1421188630491, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=551, Percent_Identity=25.0453720508167, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321610, Length=537, Percent_Identity=32.9608938547486, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6321704, Length=518, Percent_Identity=23.1660231660232, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21358483, Length=518, Percent_Identity=35.7142857142857, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24655311, Length=555, Percent_Identity=25.4054054054054, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57615; Mature: 57483

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTPFYITTAISYPNGKPHIGHAYELIATDAMARYQRLDGRDVFFLTGTDEHGQKMQQ
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
TARAEGITAQALADRNSGEFQAMAKLLNASNDDFIRTTQERHHETSRNIWNLMAENGDIY
HHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCEE
KDSYAGWYSVRDEAYYQENETELRADGVRYGPQGTPVEWVEEASYFFKLSEYQEKLLAHY
CCCCCCEEEECCHHHHCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EANPDFIGPAERRNEVISFVKSGLKDLSVSRTTFDWGIKVPNDPAHVMYVWVDALTNYIT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHE
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVFAHGFLLNKG
EEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
EKMSKSLGNVVDPVNLVSHFGLDQVRYFFLREVSFGQDGSYSEEAIGIRINSDLANGIGN
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHH
LASRSLSMIVKNCDGKIPECGVLSDEDKAMLAQADALHASTRDDMGKQQIHRALASIISV
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHH
VSETDRYFAGQAPWALKKTDPARMGTVLYVTAEVVRQIAILLQPFMPDSSGKLLDLVAAP
HHHHHHHCCCCCCCCCCCCCHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
ADKRDFAALGESGRLLAGTPLEAPTPVFPRYVAPEA
CCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDKTPFYITTAISYPNGKPHIGHAYELIATDAMARYQRLDGRDVFFLTGTDEHGQKMQQ
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
TARAEGITAQALADRNSGEFQAMAKLLNASNDDFIRTTQERHHETSRNIWNLMAENGDIY
HHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCEE
KDSYAGWYSVRDEAYYQENETELRADGVRYGPQGTPVEWVEEASYFFKLSEYQEKLLAHY
CCCCCCEEEECCHHHHCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EANPDFIGPAERRNEVISFVKSGLKDLSVSRTTFDWGIKVPNDPAHVMYVWVDALTNYIT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHE
ATGYIEDRNGPRAKYWPADVHIIGKDIIRFHAVYWPAFLMSAKLPLPKRVFAHGFLLNKG
EEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
EKMSKSLGNVVDPVNLVSHFGLDQVRYFFLREVSFGQDGSYSEEAIGIRINSDLANGIGN
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHH
LASRSLSMIVKNCDGKIPECGVLSDEDKAMLAQADALHASTRDDMGKQQIHRALASIISV
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHH
VSETDRYFAGQAPWALKKTDPARMGTVLYVTAEVVRQIAILLQPFMPDSSGKLLDLVAAP
HHHHHHHCCCCCCCCCCCCCHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
ADKRDFAALGESGRLLAGTPLEAPTPVFPRYVAPEA
CCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]