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The map label for this gene is holB [H]

Identifier: 209549398

GI number: 209549398

Start: 1852399

End: 1853424

Strand: Reverse

Name: holB [H]

Synonym: Rleg2_1804

Alternate gene names: 209549398

Gene position: 1853424-1852399 (Counterclockwise)

Preceding gene: 209549399

Following gene: 209549396

Centisome position: 40.84

GC content: 63.45

Gene sequence:

>1026_bases
ATGAGTGAAGAAAGGCCCGGACTGCTCGACGGCGCCATCTGGCCGGGCGAAAATACCAGGCTGTTCGGCCATGAGGAGGC
GCAGGCCTTCCTTGCGCAATCCTACCGGTCCGGCAAGGGTCACCACGCCATCCTGATAGAGGGGCCTGAGGGCATCGGCA
AGGCGACGCTCGCCTTCCGCTTCGCCAATCACGTGCTCTCCTATCCCGATCCTGCCACCGCGCCGGAGACGATCGGCGAT
CCGGATCCTGCCTCGTCCGTCAGCCGGCAGATCAACTCCGGCGCCTCGCATAATCTTCTGCATCTCGCCCGGCCCGTGGA
TGAAAAGACCGGCAAGGTGAAGTCGGCGATCACCGTCGACGAGGTGCGCCGCGCCGGTAAATTTTTCTCGCAGACCTCAG
GAACCGGCAATTGGCGGATTGTCATTATCGATCCGGCCGACGACATGAACCGCAATGCGGCCAACGCCATCTTGAAGATT
CTGGAAGAGCCGCCGAAGCGGGCCTTGTTCCTGGTGCTCTCGCATGCGCCGGGACGATTGCTGCCGACGATCCGCTCGCG
CTGCCTGCCGCTGAAGCTGGCGCCCCTTGCCGACGATGCGCTCATGGCGGCGCTTGCCCATCTCGGCATATCGGGCGAGG
GCGGGGCGGTGCTTTCGGCCGCCAAGGGCAGCGTCGGCGAGGCGCTGAAACTGCTGAATTACGGCGGCGGCGAGATCATT
GCCGCCTATGACGAGATGCTGTCGGCCGAAGGGCCGACGGCGCGCAAGGCGATGCATCGGCTGGCCGACGCGCTTTCGGG
GAAGGAAAGCGACACGATCTTCGATTTCTTCGTCAGCCATGTCGGCGACGACATCATGAATCGAGCACGCGCAGCGGCAG
GCGAGGGGCAGATCGCAGCTGCAGAGCGGCTGGCGCGGCTCTATTCCGAGATCACCGAACGGCTGACCATTTCGGATGCC
TATAACCTCGACCGCAAACAGACGATCATCGGCATTCTCGCCGATATCAAGCAGCCGGGTCTCTAA

Upstream 100 bases:

>100_bases
AAGCAATCGCCGCCGAGATCGTGGCGATCGTCCGGCAGCTCATGTCGCCTGCAGGCCGTGCGCGCATGCCGGAAGCCGCA
CATCATGAGTGAATCCCGTT

Downstream 100 bases:

>100_bases
TTCCGTCCTGATTCTCCGATCAGCCGGTCAGCAGCTTGACGGCGACGACGGCAAGTGTCGCGGCAAGGACGATGCGGATG
GTGCGCTCGTGCAGCTTCGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSEERPGLLDGAIWPGENTRLFGHEEAQAFLAQSYRSGKGHHAILIEGPEGIGKATLAFRFANHVLSYPDPATAPETIGD
PDPASSVSRQINSGASHNLLHLARPVDEKTGKVKSAITVDEVRRAGKFFSQTSGTGNWRIVIIDPADDMNRNAANAILKI
LEEPPKRALFLVLSHAPGRLLPTIRSRCLPLKLAPLADDALMAALAHLGISGEGGAVLSAAKGSVGEALKLLNYGGGEII
AAYDEMLSAEGPTARKAMHRLADALSGKESDTIFDFFVSHVGDDIMNRARAAAGEGQIAAAERLARLYSEITERLTISDA
YNLDRKQTIIGILADIKQPGL

Sequences:

>Translated_341_residues
MSEERPGLLDGAIWPGENTRLFGHEEAQAFLAQSYRSGKGHHAILIEGPEGIGKATLAFRFANHVLSYPDPATAPETIGD
PDPASSVSRQINSGASHNLLHLARPVDEKTGKVKSAITVDEVRRAGKFFSQTSGTGNWRIVIIDPADDMNRNAANAILKI
LEEPPKRALFLVLSHAPGRLLPTIRSRCLPLKLAPLADDALMAALAHLGISGEGGAVLSAAKGSVGEALKLLNYGGGEII
AAYDEMLSAEGPTARKAMHRLADALSGKESDTIFDFFVSHVGDDIMNRARAAAGEGQIAAAERLARLYSEITERLTISDA
YNLDRKQTIIGILADIKQPGL
>Mature_340_residues
SEERPGLLDGAIWPGENTRLFGHEEAQAFLAQSYRSGKGHHAILIEGPEGIGKATLAFRFANHVLSYPDPATAPETIGDP
DPASSVSRQINSGASHNLLHLARPVDEKTGKVKSAITVDEVRRAGKFFSQTSGTGNWRIVIIDPADDMNRNAANAILKIL
EEPPKRALFLVLSHAPGRLLPTIRSRCLPLKLAPLADDALMAALAHLGISGEGGAVLSAAKGSVGEALKLLNYGGGEIIA
AYDEMLSAEGPTARKAMHRLADALSGKESDTIFDFFVSHVGDDIMNRARAAAGEGQIAAAERLARLYSEITERLTISDAY
NLDRKQTIIGILADIKQPGL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=283, Percent_Identity=25.7950530035336, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787341, Length=165, Percent_Identity=31.5151515151515, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36366; Mature: 36235

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEERPGLLDGAIWPGENTRLFGHEEAQAFLAQSYRSGKGHHAILIEGPEGIGKATLAFR
CCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
FANHVLSYPDPATAPETIGDPDPASSVSRQINSGASHNLLHLARPVDEKTGKVKSAITVD
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHCCCHHHHHHHH
EVRRAGKFFSQTSGTGNWRIVIIDPADDMNRNAANAILKILEEPPKRALFLVLSHAPGRL
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCC
LPTIRSRCLPLKLAPLADDALMAALAHLGISGEGGAVLSAAKGSVGEALKLLNYGGGEII
CHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEE
AAYDEMLSAEGPTARKAMHRLADALSGKESDTIFDFFVSHVGDDIMNRARAAAGEGQIAA
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCHHH
AERLARLYSEITERLTISDAYNLDRKQTIIGILADIKQPGL
HHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEERPGLLDGAIWPGENTRLFGHEEAQAFLAQSYRSGKGHHAILIEGPEGIGKATLAFR
CCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
FANHVLSYPDPATAPETIGDPDPASSVSRQINSGASHNLLHLARPVDEKTGKVKSAITVD
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHCCCHHHHHHHH
EVRRAGKFFSQTSGTGNWRIVIIDPADDMNRNAANAILKILEEPPKRALFLVLSHAPGRL
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCC
LPTIRSRCLPLKLAPLADDALMAALAHLGISGEGGAVLSAAKGSVGEALKLLNYGGGEII
CHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEE
AAYDEMLSAEGPTARKAMHRLADALSGKESDTIFDFFVSHVGDDIMNRARAAAGEGQIAA
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCHHH
AERLARLYSEITERLTISDAYNLDRKQTIIGILADIKQPGL
HHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]