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The map label for this gene is yafC [H]

Identifier: 209549261

GI number: 209549261

Start: 1693377

End: 1694276

Strand: Reverse

Name: yafC [H]

Synonym: Rleg2_1663

Alternate gene names: 209549261

Gene position: 1694276-1693377 (Counterclockwise)

Preceding gene: 209549269

Following gene: 209549257

Centisome position: 37.34

GC content: 60.78

Gene sequence:

>900_bases
ATGAACGATTACAAAGCGCTGCGGACCTTTCTCCTCGCCGCCGAGAAGCGGAATTTCGCCCAGGTCGCCCGCGAACTGGA
CATGACGCCTGCGGCGGTCACGCGCGCGATCGCAGCACTTGAAGCCGAACTCGGCGTGCAGCTCTTCGTGCGCACGACCA
GGCAGGTGTCATTGACGACAGATGGCGCGATCTTCGCCGCCCAGATTCAGCCAGCGGTCAAGACGCTGGAAGAGGCGCGT
CGCGAGGTGATGAATGCCCACAAATCTGAAGAAGGGCGCCTGCGGATCAGTGCACCGACCTGGTTCGGCAAGACGGTACT
GCCGCCGATCCTGTCAGGGTTCAAGGAACGCTATCCGAAGATGAGCTTCGAGATCTCCCTGTCCGACGGTCTGGTGGACA
TCGTCGATGGCGATTATGACTTGGCAATCCGTATCTCGTCGCAGCCCTCGGACAAGTTCACGATCTGGAGAAAAATCCGG
GTGGTGCCGCGCATCCTGGTCGCTGCACCGGGCAGCCGATTTGCCACCATGGAGCACCCGAACGAACTGACACCCGACGA
CTGCCTCGCCTATAGCGGTGAAAGCCGTCGCGAGAACTGGGTCCTGTCGGACGGCGGATCCAGCATGACGATTTCCGCAG
GTAGGGCATTCAGCGCCAACAGTGGCGAGGTTCTGGCGGATATGGCCGCCGACGGCGCAGGCGTTGCCCTGTTACCAGGC
TTCAACATTGCCGATCACATCCGAAGCGGCCGCCTGATCCATGTCTTCCATGGCTGGGCGCCGCCGGATCTGTGGCTGAC
GCTATTTTATCCGCCTTACCAGGCTCTGCCACCGCGTATCGCTTCCTTCTCGAAGTTCTTCGAGGAGCAGGTTGCGGCCC
ATATGGTCATGCTGGATTGA

Upstream 100 bases:

>100_bases
GAGCGGTGGGTTCAAGCGTTGAAATCAATCTAGCCACCTTGAACGCCGACAAGAATTGCAATATTTCTTTTGATTGAATT
GCAATTATTGAAACAATGGC

Downstream 100 bases:

>100_bases
CGCATGCGGCGGTGCTTTTCGCGGATTGTGTGAGGCCCCTCCTTGCGGATTAAAGCATGTCGCGCAAAAGTGTGTAGCGG
TTTTGCGGCAACGACATGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTTDGAIFAAQIQPAVKTLEEAR
REVMNAHKSEEGRLRISAPTWFGKTVLPPILSGFKERYPKMSFEISLSDGLVDIVDGDYDLAIRISSQPSDKFTIWRKIR
VVPRILVAAPGSRFATMEHPNELTPDDCLAYSGESRRENWVLSDGGSSMTISAGRAFSANSGEVLADMAADGAGVALLPG
FNIADHIRSGRLIHVFHGWAPPDLWLTLFYPPYQALPPRIASFSKFFEEQVAAHMVMLD

Sequences:

>Translated_299_residues
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTTDGAIFAAQIQPAVKTLEEAR
REVMNAHKSEEGRLRISAPTWFGKTVLPPILSGFKERYPKMSFEISLSDGLVDIVDGDYDLAIRISSQPSDKFTIWRKIR
VVPRILVAAPGSRFATMEHPNELTPDDCLAYSGESRRENWVLSDGGSSMTISAGRAFSANSGEVLADMAADGAGVALLPG
FNIADHIRSGRLIHVFHGWAPPDLWLTLFYPPYQALPPRIASFSKFFEEQVAAHMVMLD
>Mature_299_residues
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTTDGAIFAAQIQPAVKTLEEAR
REVMNAHKSEEGRLRISAPTWFGKTVLPPILSGFKERYPKMSFEISLSDGLVDIVDGDYDLAIRISSQPSDKFTIWRKIR
VVPRILVAAPGSRFATMEHPNELTPDDCLAYSGESRRENWVLSDGGSSMTISAGRAFSANSGEVLADMAADGAGVALLPG
FNIADHIRSGRLIHVFHGWAPPDLWLTLFYPPYQALPPRIASFSKFFEEQVAAHMVMLD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=28.6689419795222, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081978, Length=301, Percent_Identity=29.9003322259136, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.1369863013699, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=27.112676056338, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=26.2068965517241, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=27.3722627737226, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=30.2788844621514, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789639, Length=256, Percent_Identity=25.390625, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788706, Length=148, Percent_Identity=33.7837837837838, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=29.96632996633, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790783, Length=254, Percent_Identity=25.5905511811024, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=247, Percent_Identity=25.5060728744939, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786448, Length=137, Percent_Identity=34.3065693430657, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=149, Percent_Identity=24.8322147651007, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33025; Mature: 33025

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTT
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCEEEEEC
DGAIFAAQIQPAVKTLEEARREVMNAHKSEEGRLRISAPTWFGKTVLPPILSGFKERYPK
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
MSFEISLSDGLVDIVDGDYDLAIRISSQPSDKFTIWRKIRVVPRILVAAPGSRFATMEHP
EEEEEEECCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEECCCCCEEECCCC
NELTPDDCLAYSGESRRENWVLSDGGSSMTISAGRAFSANSGEVLADMAADGAGVALLPG
CCCCCHHHHCCCCCCCCCCEEEECCCCEEEEECCCEECCCCCCEEEECCCCCCCEEEECC
FNIADHIRSGRLIHVFHGWAPPDLWLTLFYPPYQALPPRIASFSKFFEEQVAAHMVMLD
CCHHHHHCCCCEEEEEECCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNDYKALRTFLLAAEKRNFAQVARELDMTPAAVTRAIAALEAELGVQLFVRTTRQVSLTT
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCEEEEEC
DGAIFAAQIQPAVKTLEEARREVMNAHKSEEGRLRISAPTWFGKTVLPPILSGFKERYPK
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
MSFEISLSDGLVDIVDGDYDLAIRISSQPSDKFTIWRKIRVVPRILVAAPGSRFATMEHP
EEEEEEECCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEECCCCCEEECCCC
NELTPDDCLAYSGESRRENWVLSDGGSSMTISAGRAFSANSGEVLADMAADGAGVALLPG
CCCCCHHHHCCCCCCCCCCEEEECCCCEEEEECCCEECCCCCCEEEECCCCCCCEEEECC
FNIADHIRSGRLIHVFHGWAPPDLWLTLFYPPYQALPPRIASFSKFFEEQVAAHMVMLD
CCHHHHHCCCCEEEEEECCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]