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The map label for this gene is gloA [H]

Identifier: 209549148

GI number: 209549148

Start: 1575792

End: 1576232

Strand: Reverse

Name: gloA [H]

Synonym: Rleg2_1549

Alternate gene names: 209549148

Gene position: 1576232-1575792 (Counterclockwise)

Preceding gene: 209549151

Following gene: 209549147

Centisome position: 34.73

GC content: 60.09

Gene sequence:

>441_bases
ATGCGTTATCTCCATACAATGGTTCGCGTCAAAGACCTGGATGCCTCACTCGCCTTTTACACCACGCTGTTCGGGCTGGA
GGAGATTCGCCGGCACGAAAACGAGAAGGGCCGCTTTACCCTGGTTTTCCTGGCTGCTCGCGACGATCTCGGCCGTGCGC
GCAGCGAAAAGGCTCCCTGCCTCGAGCTTACCTATAACTGGGATACGGAAGATTATAGCGGCGGACGCAATTTCGGCCAC
CTCGCCTATGAGGTCGACGATATTTACGCAACCTGCCAGAAGCTGATGGACAACGGCATCACCATCAACCGGCCGCCGCG
CGACGGGAACATGGCTTTCGTGCGCTCGCCCGACGGCATCTCGATCGAAATCCTGCAGAAAGGCAGCCCGCTCGCCGCCG
CCGAGCCCTGGGCCTCGATGGGCAATACCGGCGCCTGGTAA

Upstream 100 bases:

>100_bases
AGTTTTCGGTTATTCCCAACTGCACATTTTATATCGTTGACTTGCCCGAGGCTGACCTCAGCTCGCCGAAAAAGCCAGAC
CCAATAAAGGAGTAGATAGA

Downstream 100 bases:

>100_bases
GGCTTTTCGCCGCAAGGCTTTTGCGAGGCTTGCGGCTTTTGCGATTTCGGGTCCGGCCGGCGCCTCGACGCTTGCCGCGA
TCCGGCCCTGCAGGCAATCT

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRYLHTMVRVKDLDASLAFYTTLFGLEEIRRHENEKGRFTLVFLAARDDLGRARSEKAPCLELTYNWDTEDYSGGRNFGH
LAYEVDDIYATCQKLMDNGITINRPPRDGNMAFVRSPDGISIEILQKGSPLAAAEPWASMGNTGAW

Sequences:

>Translated_146_residues
MRYLHTMVRVKDLDASLAFYTTLFGLEEIRRHENEKGRFTLVFLAARDDLGRARSEKAPCLELTYNWDTEDYSGGRNFGH
LAYEVDDIYATCQKLMDNGITINRPPRDGNMAFVRSPDGISIEILQKGSPLAAAEPWASMGNTGAW
>Mature_146_residues
MRYLHTMVRVKDLDASLAFYTTLFGLEEIRRHENEKGRFTLVFLAARDDLGRARSEKAPCLELTYNWDTEDYSGGRNFGH
LAYEVDDIYATCQKLMDNGITINRPPRDGNMAFVRSPDGISIEILQKGSPLAAAEPWASMGNTGAW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=38.7323943661972, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=37.1212121212121, Blast_Score=95, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323639, Length=158, Percent_Identity=33.5443037974684, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=34.4594594594595, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16494; Mature: 16494

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLHTMVRVKDLDASLAFYTTLFGLEEIRRHENEKGRFTLVFLAARDDLGRARSEKAPC
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCE
LELTYNWDTEDYSGGRNFGHLAYEVDDIYATCQKLMDNGITINRPPRDGNMAFVRSPDGI
EEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCC
SIEILQKGSPLAAAEPWASMGNTGAW
EEEEEECCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MRYLHTMVRVKDLDASLAFYTTLFGLEEIRRHENEKGRFTLVFLAARDDLGRARSEKAPC
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCE
LELTYNWDTEDYSGGRNFGHLAYEVDDIYATCQKLMDNGITINRPPRDGNMAFVRSPDGI
EEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCC
SIEILQKGSPLAAAEPWASMGNTGAW
EEEEEECCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]