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The map label for this gene is ltaE

Identifier: 209400875

GI number: 209400875

Start: 1042761

End: 1043762

Strand: Reverse

Name: ltaE

Synonym: ECH74115_1031

Alternate gene names: 209400875

Gene position: 1043762-1042761 (Counterclockwise)

Preceding gene: 209400223

Following gene: 209395955

Centisome position: 18.73

GC content: 55.99

Gene sequence:

>1002_bases
ATGATTGATTTACGCAGTGATACCGTAACCCGACCGAGCCGCGCCATGCTCGAAGCGATGATGGCCGCCCCGGTTGGGGA
CGACGTTTACGGAGACGACCCTACCGTTAATGCTCTGCAGGACTATGCAGCAGAGCTTTCCGGTAAAGAAGCCGCCATTT
TTCTGCCTACCGGCACTCAGGCCAACCTGGTCGCTCTGCTCAGTCACTGCGAACGCGGCGAAGAGTATATTGTCGGTCAG
GCCGCGCATAACTATCTGTTTGAAGCCGGTGGCGCGGCGGTGCTGGGCAGTATTCAACCGCAACCCATCGACGCGGCTGC
CGACGGCACGCTACCGCTGGATAAAGTGGCGATGAAAATCAAACCCGACGATATCCATTTCGCCCGCACCAAATTACTCA
GTCTGGAAAACACCCACAACGGCAAAGTGTTGCCGCGTGAATACCTTAAAGATGCATGGGAATTTACCCGCGAGCGCAAT
CTGGCGCTGCATGTTGACGGTGCGCGCATCTTTAATGCCGTGGTGGCTTACGGCAGCGAACTGAAAGAGCTCACGCAATA
TTGTGATTCGTTCACCATTTGCCTGTCGAAAGGTCTTGGGACGCCAGTCGGTTCATTACTCGTCGGTAATCGTGATTACA
TTAAACGTGCCATTCGCTGGCGGAAAATGACAGGTGGCGGGATGCGCCAGTCCGGCATTCTGGCTGCCGCCGGGATGTAT
GCGCTGAAAAATAACGTCGCACGGTTGCAGGAAGATCACGACAACGCCGCCTGGATGGCGGAGCAACTGCGTGAAGCAGG
CGCGGATGTGATGCGTCAGGACACTAATATGCTGTTTGTTCGCGTCGGCGAAGAAAATGCTGCCGCGTTAGGCGAATACA
TGAAAGCGAGAAACGTACTGATTAACGCCTCGCCGATTGTCCGCCTGGTGACCCATCTTGACGTCTCGCGCGAACAACTG
GCAGAAGTCGCCGCCCACTGGCGCGCATTCCTGGCGCGTTAA

Upstream 100 bases:

>100_bases
CGCAATCATCAGCGGACGCGGTGATGAAGTGATCGAACTGGCGAAAACGAACTGGCTAAGGTAAAAAGGGTGGCATTTCC
CGTCATAATAAGGACATGCC

Downstream 100 bases:

>100_bases
GGAGGGAGACGTGCCGCAACGCATTTTAGTTCTTGGTGCCAGTGGCTACATTGGTCAGCATCTGGTGCGCACACTCAGCC
AGCAAGGGCATCAGATCCTG

Product: L-threonine aldolase

Products: NA

Alternate protein names: Low-specificity L-TA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAAELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQ
AAHNYLFEAGGAAVLGSIQPQPIDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAIRWRKMTGGGMRQSGILAAAGMY
ALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQL
AEVAAHWRAFLAR

Sequences:

>Translated_333_residues
MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAAELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQ
AAHNYLFEAGGAAVLGSIQPQPIDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAIRWRKMTGGGMRQSGILAAAGMY
ALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQL
AEVAAHWRAFLAR
>Mature_333_residues
MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAAELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQ
AAHNYLFEAGGAAVLGSIQPQPIDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAIRWRKMTGGGMRQSGILAAAGMY
ALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQL
AEVAAHWRAFLAR

Specific function: Catalyzes the cleavage of L-allo-threonine and L- threonine to glycine and acetaldehyde. L-threo-phenylserine and L- erythro-phenylserine are also good substrates

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family

Homologues:

Organism=Escherichia coli, GI1787095, Length=333, Percent_Identity=98.7987987987988, Blast_Score=680, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193206522, Length=347, Percent_Identity=40.3458213256484, Blast_Score=240, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI193206520, Length=347, Percent_Identity=40.3458213256484, Blast_Score=240, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI193206524, Length=347, Percent_Identity=40.3458213256484, Blast_Score=240, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6320789, Length=285, Percent_Identity=39.2982456140351, Blast_Score=189, Evalue=5e-49,
Organism=Drosophila melanogaster, GI21355439, Length=305, Percent_Identity=41.9672131147541, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LTAE_ECO57 (P58319)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90748
- PIR:   H85598
- RefSeq:   NP_286642.1
- RefSeq:   NP_308983.1
- ProteinModelPortal:   P58319
- SMR:   P58319
- EnsemblBacteria:   EBESCT00000028118
- EnsemblBacteria:   EBESCT00000056571
- GeneID:   917698
- GeneID:   958351
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1104
- KEGG:   ecs:ECs0956
- GeneTree:   EBGT00050000010989
- HOGENOM:   HBG293912
- OMA:   QDMAAEM
- ProtClustDB:   PRK10534
- BioCyc:   ECOL83334:ECS0956-MONOMER
- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10

Pfam domain/function: PF01212 Beta_elim_lyase; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.2.5

Molecular weight: Translated: 36483; Mature: 36483

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAAELSGKEAAIFLPTGTQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
ANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQPIDAAADGTLPLDKVAMKI
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHEEEE
KPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERNLALHVDGARIFNAVVAYGSE
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
LKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAIRWRKMTGGGMRQSGILAAAGMY
HHHHHHHHHHHHHHHHCCCCCCHHHHEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHEE
INASPIVRLVTHLDVSREQLAEVAAHWRAFLAR
ECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAAELSGKEAAIFLPTGTQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
ANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQPIDAAADGTLPLDKVAMKI
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHEEEE
KPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERNLALHVDGARIFNAVVAYGSE
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
LKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAIRWRKMTGGGMRQSGILAAAGMY
HHHHHHHHHHHHHHHHCCCCCCHHHHEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHEE
INASPIVRLVTHLDVSREQLAEVAAHWRAFLAR
ECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796