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The map label for this gene is poxB [H]

Identifier: 209400223

GI number: 209400223

Start: 1043799

End: 1045517

Strand: Reverse

Name: poxB [H]

Synonym: ECH74115_1032

Alternate gene names: 209400223

Gene position: 1045517-1043799 (Counterclockwise)

Preceding gene: 209399450

Following gene: 209400875

Centisome position: 18.76

GC content: 54.39

Gene sequence:

>1719_bases
ATGAAACAAACGGTTGCAGCTTATATCGCCAAAACACTCGAATCGGCAGGCGTGAAACGCATCTGGGGAGTCACGGGCGA
CTCTCTGAACGGTCTTAGCGACAGTCTTAATCGCATGGGCACCATCGAGTGGATGTCCACCCGCCATGAAGAAGTGGCGG
CGTTTGCCGCTGGCGCTGAAGCACAACTTTCAGGAGAACTGGCGGTCTGTGCTGGATCGTGCGGCCCCGGCAACCTGCAC
TTAATCAACGGCCTGTTCGATTGCCACCGCAATCACGTTCCGGTACTGGCGATTGCCGCTCATATTCCTTCCAGCGAAAT
TGGCAGCGGCTATTTCCAGGAAACCCACCCACAAGAGCTATTCCGCGAATGTAGTCACTATTGCGAGCTGGTTTCCAGCC
CGGAGCAGATCCCACAAGTGCTGGCGATTGCCATGCGCAAAGCGGTGCTTAACCGTGGCGTTTCCGTTGTCGTGTTACCG
GGCGACGTGGCGTTAAAACCTGCGCCAGAAGGGGCAACTACCCACTGGTATCATGCGCCACAGCCAGTCGTGACGCCGGA
AGAAGAAGAGTTACGCAAACTGGCGCAACTGCTGCGTTATTCCAGCAATATCGCTCTGATGTGTGGCAGCGGCTGCGCGG
GGGCGCATAAAGAGTTAGTTGAGTTTGCCGGGAAAATTAAAGCACCTATTGTTCATGCCCTACGCGGTAAAGAGCATGTC
GAATACGATAATCCGTATGATGTTGGAATGACCGGGTTAATCGGATTCTCGTCAGGTTTCCATACCATGATGAACGCCGA
CACGTTAGTGCTACTCGGTACTCAGTTCCCCTACCGTGCGTTCTACCCGACCGATGCCAAAATTATTCAGATTGATATCA
ACCCAGCCAGCATCGGCGCACACAGCAAGGTGGATATGGCGCTGGTCGGCGATATCAAGTCGACCCTGCGTGCATTGCTG
CCATTGGTGGAAGAAAAAGCCGATCGCAAGTTTCTGGATAAAGCGCTGGAAGATTACCGCGATGCCCGCAAAGGGCTGGA
CGATTTAGCTAAACCGAGCGAGAAAGCCATTCACCCGCAATATCTGGCGCAGCAAATTAGTCATTTTGCCGCCGATGACG
CCATCTTTACCTGTGACGTCGGCACGCCAACGGTGTGGGCGGCGCGTTATCTGAAAATGAACGGCAAGCGTCGCCTGTTA
GGTTCGTTTAACCACGGTTCGATGGCTAACGCCATGCCGCAGGCGCTGGGCGCGCAGGCAACCGAGCCGGAACGTCAGGT
GGTCGCCATGTGCGGCGATGGCGGTTTTAGCATGTTGATGGGCGATTTCCTCTCGGTAGTGCAGATGAAACTGCCGGTGA
AAATTATCGTCTTTAATAACAGCGTGCTGGGCTTTGTGGCGATGGAGATGAAAGCCGGAGGCTACCTGACAGACGGTACT
GAGCTGCACGACACCAACTTTGCCCGCATTGCCGAAGCGTGCGGAATTACGGGTATTCGTGTAGAAAAAGCCTCTGAAAT
CGATGAAGCCCTGCAACGCGCCTTCTCCATCGACGGTCCGGTATTGGTGGATGTGGTGGTCGCCAAAGAAGAGTTAGCCA
TTCCACCGCAGATCAAACTCGAACAGGCCAAAGGTTTCAGCCTGTATATGCTGCGCGCAATCATCAGCGGACGCGGTGAT
GAAGTGATCGAACTGGCGAAAACGAACTGGCTAAGGTAA

Upstream 100 bases:

>100_bases
GGCTATTTAACCGTTAGTGCCTCCTTTCTCTCCCATCCCTTCCCCCTCCGTCAGATGAACTAAACTTGTTACCGTTATCA
CATTCAGGAGATGGAGAACC

Downstream 100 bases:

>100_bases
AAAGGGTGGCATTTCCCGTCATAATAAGGACATGCCATGATTGATTTACGCAGTGATACCGTAACCCGACCGAGCCGCGC
CATGCTCGAAGCGATGATGG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKADRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Sequences:

>Translated_572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKADRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR
>Mature_572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKADRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=23.7924865831843, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=23.3788395904437, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=99.4755244755245, Blast_Score=1180, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=30.1785714285714, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=28.6751361161525, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=25.3649635036496, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788716, Length=540, Percent_Identity=22.7777777777778, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=26.2969588550984, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=26.2969588550984, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=25.4480286738351, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320816, Length=540, Percent_Identity=23.1481481481481, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922626, Length=553, Percent_Identity=24.4122965641953, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62010; Mature: 62010

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHCCCC
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCEECC
QPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEECCHHCCCC
HSKVDMALVGDIKSTLRALLPLVEEKADRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHCCCC
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCEECC
QPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEECCHHCCCC
HSKVDMALVGDIKSTLRALLPLVEEKADRKFLDKALEDYRDARKGLDDLAKPSEKAIHPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]