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The map label for this gene is waaA

Identifier: 209400697

GI number: 209400697

Start: 4655503

End: 4656780

Strand: Direct

Name: waaA

Synonym: ECH74115_5003

Alternate gene names: 209400697

Gene position: 4655503-4656780 (Clockwise)

Preceding gene: 209399220

Following gene: 209395850

Centisome position: 83.55

GC content: 53.91

Gene sequence:

>1278_bases
ATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAA
GGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGC
ACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCTATT
ACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCC
GTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTAT
GGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCC
GCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGA
TGGTGCACGTTTTGTGGCGCTGGGCGCGAAAAATAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAA
CGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACT
CACGAAGGTGAAGAGAGCGTGGTCATTGCCGCACATCAGGCTTTATTACAGCAATTCCCGAATTTATTGCTCATCCTGGT
ACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAG
GGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGAT
CTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCAATTCCGGTATT
GATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATG
CCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAA
GTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGA

Upstream 100 bases:

>100_bases
GCGGGTAAATTGCTATTTAAGTCAAAAACGGGAAAAGTAATGGTAAAGCCACAGCTAAATACATAGAATCCCCAGCACAT
CCATAAGTCAGCTATTTACT

Downstream 100 bases:

>100_bases
GGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCG
CCACGCAGATGTTCGATCAC

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPI
TVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIAD
LAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVE
VLYQNQGALQRLLQLLEPYLPPKTH

Sequences:

>Translated_425_residues
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPI
TVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIAD
LAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVE
VLYQNQGALQRLLQLLEPYLPPKTH
>Mature_425_residues
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPI
TVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIAD
LAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVE
VLYQNQGALQRLLQLLEPYLPPKTH

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

Organism=Escherichia coli, GI1790064, Length=425, Percent_Identity=100, Blast_Score=863, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDTA_ECO57 (P0AC77)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D98192
- RefSeq:   NP_290213.1
- RefSeq:   NP_312535.1
- ProteinModelPortal:   P0AC77
- MINT:   MINT-1234716
- EnsemblBacteria:   EBESCT00000025472
- EnsemblBacteria:   EBESCT00000059671
- GeneID:   915541
- GeneID:   960913
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5057
- KEGG:   ecs:ECs4508
- GeneTree:   EBGT00050000009558
- HOGENOM:   HBG664037
- OMA:   HPQRFDE
- ProtClustDB:   PRK05749
- BioCyc:   ECOL83334:ECS4508-MONOMER
- InterPro:   IPR001296
- InterPro:   IPR007507

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N

EC number: 2.-.-.-

Molecular weight: Translated: 47292; Mature: 47292

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11f490fc)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
TLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
VDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGKFVRRLLRRITLI
CCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHH
AAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
EECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECC
HEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTS
CCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCC
TQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICA
CEEEECCCHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHEEECCCCCCHHHHHH
RLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVEVLYQNQGALQRLLQLLEPYL
HHHHCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PPKTH
CCCCH
>Mature Secondary Structure
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
TLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
VDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGKFVRRLLRRITLI
CCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHH
AAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
EECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECC
HEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTS
CCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCC
TQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICA
CEEEECCCHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHEEECCCCCCHHHHHH
RLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVEVLYQNQGALQRLLQLLEPYL
HHHHCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PPKTH
CCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796