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The map label for this gene is napB

Identifier: 209400531

GI number: 209400531

Start: 3073184

End: 3073633

Strand: Reverse

Name: napB

Synonym: ECH74115_3340

Alternate gene names: 209400531

Gene position: 3073633-3073184 (Counterclockwise)

Preceding gene: 209397253

Following gene: 209398459

Centisome position: 55.16

GC content: 56.0

Gene sequence:

>450_bases
ATGAAAAGCCATGACCTGAAGAAAGCGCTGTGTCAATGGACGGCGATGCTGGCCCTGGTGGTAAGCGGCGCGGTTTGGGC
GGCTAATGGCGTCGATTTTAGCCAGTCGCCGGAAGTATCCGGGACGCAGGAAGGGGCCATTCGGATGCCAAAAGAGCAGG
ATCGGATGCCGCTGAACTATGTGAATCAGCCGCCAATGATCCCGCATAGCGTTGAAGGTTATCAGGTAACGACCAATACC
AACCGCTGCTTGCAGTGCCACGGTGTCGAAAGCTATCGCACCACTGGCGCGCCGCGTATCAGTCCTACTCACTTTATGGA
CAGCGACGGCAAAGTGGGCGCGGAAGTGGCTCCGCGTCGTTATTTCTGTCTGCAATGTCACGTACCGCAGGCCGATACCG
CGCCAATCGTGGGGAATACCTTTACCCCATCAAAAGGTTACGGGAAATAA

Upstream 100 bases:

>100_bases
AGGTCACCAGCCGCGATTGCATGACGTGCGGTCGCTGCGTGGATGTCTGTTCTGAGGATGTATTTACAATAACTACACGA
TGGAGTTCGGGAGCGAAATC

Downstream 100 bases:

>100_bases
GAGGTTATTATGGGAAATTCTGACCGTAAGCCTGGTCTGATTAAGCGCCTGTGGAAATGGTGGCGTACCCCCAGCCGTCT
GGCGCTGGGGACGCTGCTGT

Product: citrate reductase cytochrome c-type subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNT
NRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK

Sequences:

>Translated_149_residues
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNT
NRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK
>Mature_149_residues
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNT
NRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=149, Percent_Identity=100, Blast_Score=317, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPB_ECOLI (P0ABL3)

Other databases:

- EMBL:   U00008
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_002799.1
- RefSeq:   NP_416707.4
- ProteinModelPortal:   P0ABL3
- SMR:   P0ABL3
- STRING:   P0ABL3
- EnsemblBacteria:   EBESCT00000003539
- EnsemblBacteria:   EBESCT00000003540
- EnsemblBacteria:   EBESCT00000003541
- EnsemblBacteria:   EBESCT00000014882
- GeneID:   946698
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5367
- KEGG:   eco:b2203
- EchoBASE:   EB1991
- EcoGene:   EG12061
- eggNOG:   COG3043
- GeneTree:   EBGT00050000008929
- HOGENOM:   HBG321219
- OMA:   CHGVENY
- ProtClustDB:   PRK11586
- BioCyc:   EcoCyc:NAPB-MONOMER
- BioCyc:   MetaCyc:NAPB-MONOMER
- Genevestigator:   P0ABL3
- GO:   GO:0006810
- InterPro:   IPR011031
- InterPro:   IPR005591

Pfam domain/function: PF03892 NapB

EC number: NA

Molecular weight: Translated: 16297; Mature: 16297

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 83-83 BINDING 86-86 BINDING 123-123 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQDRMPLNY
CCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
VNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRR
CCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHCCCCCCCCCCCEECCCCCCCCCCCCCE
YFCLQCHVPQADTAPIVGNTFTPSKGYGK
EEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQDRMPLNY
CCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
VNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRR
CCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHCCCCCCCCCCCEECCCCCCCCCCCCCE
YFCLQCHVPQADTAPIVGNTFTPSKGYGK
EEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8039676