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The map label for this gene is napC

Identifier: 209398459

GI number: 209398459

Start: 3072572

End: 3073174

Strand: Reverse

Name: napC

Synonym: ECH74115_3339

Alternate gene names: 209398459

Gene position: 3073174-3072572 (Counterclockwise)

Preceding gene: 209400531

Following gene: 209396482

Centisome position: 55.15

GC content: 51.58

Gene sequence:

>603_bases
ATGGGAAATTCTGACCGTAAGCCTGGTCTGATTAAGCGCCTGTGGAAATGGTGGCGTACCCCCAGCCGTCTGGCGCTGGG
GACGCTGCTGTTGATCGGTTTTGTCGGCGGCATCGTCTTCTGGGGCGGCTTTAACACTGGGATGGAAAAAGCCAATACCG
AAGAGTTCTGCATTAGCTGCCACGAAATGCGTAACACGGTGTATCAGGAATACATGGATTCCGTGCACTACAACAACCGT
AGCGGCGTCCGTGCGACCTGTCCGGATTGTCACGTTCCGCACGAGTTTGTGCCGAAGATGATACGCAAGCTCAAAGCAAG
TAAAGAGCTGTATGGTAAAATTTTTGGCGTTATTGACACGCCGCAGAAATTTGAAGCTCATCGTCTGACGATGGCACAGA
ATGAGTGGCGGCGCATGAAGGACAATAACTCGCAGGAGTGCCGTAACTGTCACAACTTCGAGTATATGGATACAACCGCC
CAGAAATCGGTTGCCGCGAAGATGCATGACCAGGCGGTGAAAGATGGGCAAACCTGTATTGATTGCCATAAAGGGATAGC
GCACAAGCTGCCCGATATGCGTGAAGTCGAGCCAGGTTTTTAA

Upstream 100 bases:

>100_bases
TTATTTCTGTCTGCAATGTCACGTACCGCAGGCCGATACCGCGCCAATCGTGGGGAATACCTTTACCCCATCAAAAGGTT
ACGGGAAATAAGAGGTTATT

Downstream 100 bases:

>100_bases
CAGGGTTATTGCGTGGGTATGCTTGAAGTCAGAGAGTTACTTTGTGAGCGGGATGAACGAACCTTATTTAGTGGCTTGTC
ATTTACGCTGAACGCAGGAG

Product: cytochrome c-type protein NapC

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNR
SGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF

Sequences:

>Translated_200_residues
MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNR
SGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF
>Mature_199_residues
GNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRS
GVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQ
KSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF

Specific function: Mediates electron flow from quinones to the NapAB complex

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=200, Percent_Identity=100, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787230, Length=186, Percent_Identity=50, Blast_Score=184, Evalue=3e-48,
Organism=Escherichia coli, GI1788181, Length=148, Percent_Identity=45.945945945946, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPC_ECO57 (P0ABL7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91015
- PIR:   E85859
- RefSeq:   NP_288782.1
- RefSeq:   NP_311118.1
- ProteinModelPortal:   P0ABL7
- SMR:   P0ABL7
- EnsemblBacteria:   EBESCT00000026013
- EnsemblBacteria:   EBESCT00000058133
- GeneID:   916797
- GeneID:   960378
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3459
- KEGG:   ecs:ECs3091
- GeneTree:   EBGT00050000010586
- HOGENOM:   HBG297153
- OMA:   EKFKERR
- ProtClustDB:   PRK10617
- BioCyc:   ECOL83334:ECS3091-MONOMER
- GO:   GO:0006810
- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885
- TIGRFAMs:   TIGR02161

Pfam domain/function: PF03264 Cytochrom_NNT

EC number: NA

Molecular weight: Translated: 23101; Mature: 22970

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 57-57 BINDING 60-60 BINDING 87-87 BINDING 90-90 BINDING 147-147 BINDING 150-150 BINDING 179-179 BINDING 182-182

Signals:

None

Transmembrane regions:

HASH(0x409fba60)-;

Cys/Met content:

4.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISC
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDT
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQKSVAAKMHDQAVKDGQTCI
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
DCHKGIAHKLPDMREVEPGF
HHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure 
GNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISC
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDT
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQKSVAAKMHDQAVKDGQTCI
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
DCHKGIAHKLPDMREVEPGF
HHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796