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The map label for this gene is metQ

Identifier: 209400462

GI number: 209400462

Start: 223424

End: 224239

Strand: Reverse

Name: metQ

Synonym: ECH74115_0208

Alternate gene names: 209400462

Gene position: 224239-223424 (Counterclockwise)

Preceding gene: 209399723

Following gene: 209397672

Centisome position: 4.02

GC content: 48.65

Gene sequence:

>816_bases
ATGGCGTTCAAATTCAAAACCTTTGCGGCAGTGGGAGCCCTGATTGGATCACTGGCACTGGTAGGCTGCGGTCAGGATGA
AAAAGATCCAAACCACATTAAAGTCGGCGTGATTGTTGGTGCCGAACAGCAGGTTGCAGAAGTCGCGCAGAAAGTTGCGA
AAGACAAATATGGCCTGGACGTTGAGCTGGTAACCTTCAACGACTATGTTCTGCCAAACGAAGCATTGAGCAAAGGCGAT
ATCGACGCCAACGCCTTCCAGCATAAACCGTACCTTGATCAGCAACTGAAAGATCGTGGCTACAAACTGGTCGCAGTAGG
CAACACTTTTGTTTATCCGATTGCTGGTTACTCCAAGAAAATCAAATCACTGGATGAACTGCAGGATGGTTCGCAGGTTG
CCGTGCCAAACGATCCAACTAACCTTGGTCGTTCACTGCTGCTGCTGCAAAAAGTGGGCTTGATCAAACTGAAAGATGGC
GTTGGCCTGCTGCCGACCGTTCTTGATGTTGTTGAGAACCCAAAAAATCTGAAAATTGTTGAACTGGAAGCACCGCAACT
GCCGCGTTCTCTGGACGACGCGCAAATCGCTCTGGCAGTTATTAACACCACCTATGCCAGCCAGATTGGCCTGACTCCAG
CGAAAAACGGTATCTTTGTCGAAGATAAAGACTCCCCGTACGTAAACCTGATCGTAACGCGTGAAGATAACAAAGACGCC
GAGAACGTGAAGAAATTCGTCCAGGCTTATCAGTCTGACGAAGTTTACGAAGCAGCAAACAAAGTGTTTAACGGCGGCGC
TGTTAAAGGCTGGTAA

Upstream 100 bases:

>100_bases
TCTGGTTTATTTAATTCAGTTCGCAGGCGACCGCATCGTCCGGGCTGTCACTCGCAAGTAACGTTCAACACAACATAAAT
AATTGAAGAAGGAATAAGGT

Downstream 100 bases:

>100_bases
TTTTTAGGCTGTTTCCACAATTTGTAATATCATTCAGGACGGGCGCTTGCCCGTCTTGTCATTTTTACAAGCTCCTGATT
CAATATTGACGTTTTGATCA

Product: DL-methionine transporter substrate-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGD
IDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGIFVEDKDSPYVNLIVTREDNKDA
ENVKKFVQAYQSDEVYEAANKVFNGGAVKGW

Sequences:

>Translated_271_residues
MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGD
IDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGIFVEDKDSPYVNLIVTREDNKDA
ENVKKFVQAYQSDEVYEAANKVFNGGAVKGW
>Mature_270_residues
AFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDI
DANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDGV
GLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGIFVEDKDSPYVNLIVTREDNKDAE
NVKKFVQAYQSDEVYEAANKVFNGGAVKGW

Specific function: This protein is a component of a D-methionine permease, a binding protein-dependent, ATP-driven transport system

COG id: COG1464

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface antigen

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpA lipoprotein family

Homologues:

Organism=Escherichia coli, GI1786396, Length=271, Percent_Identity=99.2619926199262, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790093, Length=249, Percent_Identity=63.4538152610442, Blast_Score=340, Evalue=5e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METQ_ECO57 (Q8X8V9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G85504
- PIR:   G90653
- RefSeq:   NP_285891.1
- RefSeq:   NP_308226.1
- ProteinModelPortal:   Q8X8V9
- SMR:   Q8X8V9
- EnsemblBacteria:   EBESCT00000028201
- EnsemblBacteria:   EBESCT00000060429
- GeneID:   913959
- GeneID:   956942
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0209
- KEGG:   ecs:ECs0199
- GeneTree:   EBGT00050000009441
- HOGENOM:   HBG661773
- OMA:   TYASQIN
- ProtClustDB:   PRK11063
- BioCyc:   ECOL83334:ECS0199-MONOMER
- InterPro:   IPR004872
- InterPro:   IPR004478
- TIGRFAMs:   TIGR00363

Pfam domain/function: PF03180 Lipoprotein_9

EC number: NA

Molecular weight: Translated: 29417; Mature: 29286

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLD
CCEEHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCE
VELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKK
EEEEEECCEECCCHHHCCCCCCCCHHCCCCCHHHHHHHCCEEEEEECCEEEEECCCHHHH
IKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDGVGLLPTVLDVVENPKNLKIV
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCEEEE
ELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGIFVEDKDSPYVNLIVTREDNKDA
EECCCCCCCCCCCCEEEEEEEEHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCCH
ENVKKFVQAYQSDEVYEAANKVFNGGAVKGW
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLD
CEEHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCE
VELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKK
EEEEEECCEECCCHHHCCCCCCCCHHCCCCCHHHHHHHCCEEEEEECCEEEEECCCHHHH
IKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDGVGLLPTVLDVVENPKNLKIV
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCEEEE
ELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGIFVEDKDSPYVNLIVTREDNKDA
EECCCCCCCCCCCCEEEEEEEEHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCCH
ENVKKFVQAYQSDEVYEAANKVFNGGAVKGW
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796