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The map label for this gene is metI

Identifier: 209399723

GI number: 209399723

Start: 224279

End: 224932

Strand: Reverse

Name: metI

Synonym: ECH74115_0209

Alternate gene names: 209399723

Gene position: 224932-224279 (Counterclockwise)

Preceding gene: 209399329

Following gene: 209400462

Centisome position: 4.04

GC content: 52.14

Gene sequence:

>654_bases
ATGTCTGAGCCGATGATGTGGCTGCTGGTTCGTGGCGTATGGGAAACGCTGGCAATGACCTTCGTATCCGGATTTTTTGG
GTTTGTGGTTGGTCTGCCGGTTGGCGTCCTGCTTTATGTCACGCGTCCGGGGCAAATTATTGCTAACGCGAAGCTGTATC
GTACCGTTTCTGCGATTGTGAACATTTTCCGTTCCATCCCGTTCATTATCTTGCTGGTATGGATGATTCCGTTTACCCGC
GTTATTGTCGGTACATCGATTGGTTTGCAGGCAGCAATTGTTCCGTTAACCGTTGGTGCAGCACCGTTTATTGCCCGTAT
GGTCGAGAACGCTCTGCTGGAGATCCCAACCGGGTTAATTGAAGCTTCCCGCGCAATGGGTGCCACGCCAATGCAGATCG
TCCGTAAAGTGCTGTTACCGGAAGCGTTGCCGGGTCTGGTGAATGCGGCAACTATCACCCTGATTACCCTGGTCGGTTAT
TCCGCGATGGGTGGTGCAGTCGGTGCCGGTGGTTTAGGTCAGATTGGCTATCAGTATGGCTACATCGGCTATAACGCGAC
GGTGATGAATACGGTACTGGTATTGCTGGTCATTCTGGTTTATTTAATTCAGTTCGCAGGCGACCGCATCGTCCGGGCTG
TCACTCGCAAGTAA

Upstream 100 bases:

>100_bases
TTCGGCATCATGCTGACTGAAATGCACGGCACACAACAAGATACGCAAGCCGCCATTGCCTGGCTGCAGGAACACCATGT
AAAAGTAGAGGTACTGGGTT

Downstream 100 bases:

>100_bases
CGTTCAACACAACATAAATAATTGAAGAAGGAATAAGGTATGGCGTTCAAATTCAAAACCTTTGCGGCAGTGGGAGCCCT
GATTGGATCACTGGCACTGG

Product: DL-methionine transporter permease subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTR
VIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK

Sequences:

>Translated_217_residues
MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTR
VIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK
>Mature_216_residues
SEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRV
IVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYS
AMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK

Specific function: Part of the binding-protein-dependent transport system for D-methionine and the toxic methionine analog alpha-methyl- methionine. Probably responsible for the translocation of the substrate across the membrane

COG id: COG2011

COG function: function code P; ABC-type metal ion transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1786397, Length=217, Percent_Identity=99.5391705069124, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METI_ECO57 (Q8X800)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H85504
- PIR:   H90653
- RefSeq:   NP_285892.1
- RefSeq:   NP_308227.1
- ProteinModelPortal:   Q8X800
- SMR:   Q8X800
- EnsemblBacteria:   EBESCT00000027198
- EnsemblBacteria:   EBESCT00000057802
- GeneID:   913960
- GeneID:   956943
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0210
- KEGG:   ecs:ECs0200
- GeneTree:   EBGT00050000008565
- HOGENOM:   HBG592565
- OMA:   YLIQFSG
- ProtClustDB:   PRK10782
- BioCyc:   ECOL83334:ECS0200-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 23243; Mature: 23111

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x24f790f8)-; HASH(0x24e1c830)-; HASH(0x24568ed8)-; HASH(0x23a2adb0)-; HASH(0x24747fc0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPGQIIANAKLYRTVSAIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHH
NIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHH
EASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
YIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPGQIIANAKLYRTVSAIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHH
NIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHH
EASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
YIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796