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The map label for this gene is melA [H]

Identifier: 209400354

GI number: 209400354

Start: 5271560

End: 5272915

Strand: Direct

Name: melA [H]

Synonym: ECH74115_5633

Alternate gene names: 209400354

Gene position: 5271560-5272915 (Clockwise)

Preceding gene: 209400808

Following gene: 209396194

Centisome position: 94.61

GC content: 52.95

Gene sequence:

>1356_bases
ATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCA
TCGCGAGGCGCTGAAAACGGCGCATATTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGC
GTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTACAGGATGCCGAT
TTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGCACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCT
GGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTT
GCGAGGACATGACGGAAGTCTGCCCCGATGCCACCATGCTCAACTATGTTAACCCAATGGCGATGAATACCTGGGCGATG
TATGCCCGCTATCCGCATATCAAACAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGCTGGCGCGTGACCT
CAATATCGACCCGGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAA
CCGCCGACGGCAGTTACGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGACGCAGGGCAGGCACCGAAGCCGAATATT
CACGGCAATACTCGCTGCCAGAATATTGTGCGCTATGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGA
ACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATG
AGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAACGCCTCCCGGATTGAT
ATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACGGGCGAGCCGAGTGTGATTTACGGCAACGT
CCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTGGCCTGTCTGGTTGATGCTAATGGCATTC
AGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACC
GAAGCTATTCTTACGGAAAATCGCGACCGTGTTTACCACGCTGCGATGATGGACCCGCATACTGCCGCCGTGCTGGGCAT
TGACGAAATATATGCTCTTGTTGACGACCTGATTGCCGCCCACGGCGACTGGCTGCCAGGCTGGTTGCACCGTTAA

Upstream 100 bases:

>100_bases
GCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTC
AAGCAAGCCAGGAGATCTGC

Downstream 100 bases:

>100_bases
AACGCGACTAAACGCTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATACCAATAACAGAAGGCGGGCGTT
GGTAACAGCGACCCGATACC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYAPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYDAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR

Sequences:

>Translated_451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYAPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYDAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
>Mature_451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYAPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYDAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=451, Percent_Identity=99.1130820399113, Blast_Score=939, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=460, Percent_Identity=25.6521739130435, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50571; Mature: 50571

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYDAGQAPKPNI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYDAGQAPKPNI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]