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The map label for this gene is melB [H]

Identifier: 209396194

GI number: 209396194

Start: 5272994

End: 5274439

Strand: Direct

Name: melB [H]

Synonym: ECH74115_5634

Alternate gene names: 209396194

Gene position: 5272994-5274439 (Clockwise)

Preceding gene: 209400354

Following gene: 209397932

Centisome position: 94.63

GC content: 46.2

Gene sequence:

>1446_bases
TTGGTAACAGCGACCCGATACCCTATGAGCATTTCAATGACTACAAAACTCAGTTATGGATTTGGAGCGTTCGGGAAGGA
TTTTGCGATCGGCATTGTGTATATGTACCTCATGTATTACTACACCGATGTCGTCGGGCTGTCTGTGGGTTTGGTCGGTA
CTTTGTTTCTGGTGGCGAGGATCTGGGATGCTATTAACGATCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGA
TGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGTTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCT
GTTTGAAGGTACTACTCAGATTGCCTTTGTTTGCGTGACCTACATCCTCTGGGGCATGACTTACACCATTATGGATATTC
CCTTCTGGTCGCTGGTTCCAACCATCACGCTCGATAAACGTGAGCGTGAACAACTGGTTCCTTATCCGCGTTTTTTTGCC
AGTCTGGCGGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTT
TCAGATGTTCACACTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTT
CGTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTTATTTATAAAAACGATCAG
CTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTC
ATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCT
TCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTT
CTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGC
GCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAA
GCATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGG
ATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCGAC
TCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGCGACACGCTGCGCAGGATCCAGATTC
ATCTGCTGGATAAATATCGCAAAATACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAA
GCCTGA

Upstream 100 bases:

>100_bases
GCCAGGCTGGTTGCACCGTTAAAACGCGACTAAACGCTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATA
CCAATAACAGAAGGCGGGCG

Downstream 100 bases:

>100_bases
CGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGGAATGCTTTGGATTTGCAA
ATTTTGTAGACCGGATAAGG

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MVTATRYPMSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSR
WGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFA
SLAGFVTAGVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQ
LSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGV
LLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKIPPEPVHADIPVGAVSDVK
A

Sequences:

>Translated_481_residues
MVTATRYPMSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSR
WGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFA
SLAGFVTAGVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQ
LSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGV
LLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKIPPEPVHADIPVGAVSDVK
A
>Mature_481_residues
MVTATRYPMSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSR
WGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFA
SLAGFVTAGVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQ
LSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGV
LLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKIPPEPVHADIPVGAVSDVK
A

Specific function: Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system) [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=469, Percent_Identity=99.3603411513859, Blast_Score=942, Evalue=0.0,
Organism=Escherichia coli, GI87082306, Length=469, Percent_Identity=28.1449893390192, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786466, Length=467, Percent_Identity=27.8372591006424, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI48994989, Length=448, Percent_Identity=26.1160714285714, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693206, Length=457, Percent_Identity=25.8205689277899, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787902, Length=465, Percent_Identity=27.3118279569892, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 53531; Mature: 53531

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTATRYPMSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVAR
CCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIAFVCVT
HHHHHCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNYVGG
HHHHHHHHHHHHCCHHHHCCCEEECCHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCC
GDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
LSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
LSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQF
CCCCCEEEEEEHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKIPPEPVHADIPVGAVSDVK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
A
C
>Mature Secondary Structure
MVTATRYPMSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVAR
CCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIAFVCVT
HHHHHCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNYVGG
HHHHHHHHHHHHCCHHHHCCCEEECCHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCC
GDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
LSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
LSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQF
CCCCCEEEEEEHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKIPPEPVHADIPVGAVSDVK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6323466; 7610040; 9278503; 1730719; 8672452; 1872836 [H]