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The map label for this gene is cysN [H]

Identifier: 209400153

GI number: 209400153

Start: 3698912

End: 3700339

Strand: Reverse

Name: cysN [H]

Synonym: ECH74115_4003

Alternate gene names: 209400153

Gene position: 3700339-3698912 (Counterclockwise)

Preceding gene: 209400969

Following gene: 209399413

Centisome position: 66.41

GC content: 54.13

Gene sequence:

>1428_bases
ATGAACACCGCACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATTGCGCAACAACATAAAAGCCT
GCTGCGTTTTCTGACCTGTGGTAGCGTCGATGACGGCAAAAGTACCCTGATTGGTCGTCTGTTGCACGATACCCGCCAAA
TCTATGAAGATCAGCTCTCATCGCTGCATAATGACAGTAAGCGTCACGGCACCCAGGGCGAAAAGCTGGATCTGGCTCTG
CTGGTGGACGGCCTGCAAGCTGAGCGTGAACAGGGCATCACCATTGACGTGGCCTACCGCTATTTCTCTACCGAGAAGCG
TAAATTTATTATCGCCGACACTCCAGGGCACGAGCAGTACACCCGCAATATGGCGACTGGCGCATCGACATGTGAACTGG
CGATCTTACTGATCGATGCCCGTAAAGGCGTGCTCGATCAAACCCGTCGTCACAGTTTTATCTCCACACTGTTGGGGATC
AAACATCTGGTCGTGGCGATCAACAAAATGGATCTGGTGGATTACAGTGAAGAGACGTTCACCCGTATTCGTGAAGATTA
TCTGACCTTTGCCGGGCAGCTGCCGGGTAATCTGGATATCCGCTTTGTGCCGCTCTCCGCACTGGAAGGTGACAACGTGG
CTTCGCAAAGTGAAAGTATGCCGTGGTACAGCGGTCCGACACTGCTCGAAGTGCTGGAAACCGTGGAGATTCAGCGAGTG
GTGGATGCCCAGCCAATGCGCTTCCCGGTGCAGTACGTTAACCGCCCAAATCTCGATTTTCGTGGCTACGCCGGAACGCT
GGCATCCGGTCGCGTGGAAGTCGGGCAACGTGTAAAAGTGCTGCCCTCTGGTGTGGAATCAAACGTCGCGCGGATCGTGA
CTTTTGATGGTGATCGCGAAGAAGCCTTTGCCGGAGAAGCTATCACCCTGGTGCTGACGGATGAGATCGACATCAGCCGT
GGCGATCTGCTGCTGGCGGCAGACGAAGCGTTACCAGCTGTGCAGAGCGCGTCGGTGGATGTGGTATGGATGGCGGAACA
GCCGCTTTCCCCGGGCCAGAGTTACGACATCAAAATTGCCGGTAAGAAGACGCGTTCTCGTGTTGATGGCATTCGTTATC
AGGTTGATATTAATAACCTTACCCAACGCGAAGTTGAAAACCTGCCGCTGAACGGCATCGGCCTGGTGGATCTCACTTTT
GACGAGCCACTGGTGTTAGATCGTTATCAGCAAAACCCGGTTACCGGTGGGCTGATTTTTATCGATCGCCTGAGCAATGT
GACCGTAGGTGCCGGTATGGTGCATGAGCCAGTTAGCCAGGCAACTGCTGCGCCATCTGAATTTAGTGCATTCGAACTGG
AATTGAATGCCCTGGTTCGCCGCCACTTTCCACACTGGGGCGCGCGCGATTTGCTGGGGGAGAAATAA

Upstream 100 bases:

>100_bases
GAGATGCTGGTTTCCACCACCAGTGAACGTCAGGGGCGCGTGATTGACCGCGACCAAGCTGGTTCTATGGAGCTGAAAAA
ACGTCAGGGGTATTTTTAAG

Downstream 100 bases:

>100_bases
TGGCGCTGCATGACGAAAACGTCGTCTGGCATAGCCATCCGGTCACTGTGCAACAACGCGAGCTACACCACGGTCATCGT
GGTGTAGTGCTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGEK

Sequences:

>Translated_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGEK
>Mature_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGEK

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=28.9655172413793, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=28.9655172413793, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI194097354, Length=293, Percent_Identity=34.1296928327645, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI194018520, Length=293, Percent_Identity=34.1296928327645, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI194018522, Length=293, Percent_Identity=34.1296928327645, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=27.765237020316, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=26.86230248307, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=33.1058020477816, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI34147630, Length=305, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=351, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=99.5789473684211, Blast_Score=969, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=326, Percent_Identity=26.9938650306748, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790412, Length=326, Percent_Identity=26.9938650306748, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367247, Length=293, Percent_Identity=26.6211604095563, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532067, Length=342, Percent_Identity=29.5321637426901, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI115532065, Length=342, Percent_Identity=29.5321637426901, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=29.2134831460674, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=29.2134831460674, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=28.4037558685446, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.8538812785388, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=26.9709543568465, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=348, Percent_Identity=28.735632183908, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=393, Percent_Identity=29.7709923664122, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=27.891156462585, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=27.891156462585, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=28.1464530892449, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=34.2465753424658, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=149, Percent_Identity=34.2281879194631, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=28.5388127853881, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45553807, Length=276, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553816, Length=276, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24651721, Length=276, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864154, Length=276, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=26.3038548752834, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=26.3038548752834, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17137380, Length=315, Percent_Identity=30.7936507936508, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281363316, Length=316, Percent_Identity=30.0632911392405, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17864358, Length=316, Percent_Identity=30.0632911392405, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921738, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922690, Length=289, Percent_Identity=25.9515570934256, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52589; Mature: 52589

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRSRVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHHHCCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGEK
EEECCCCEECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRSRVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHHHCCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGEK
EEECCCCEECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA