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The map label for this gene is pitB [H]

Identifier: 209399859

GI number: 209399859

Start: 3978491

End: 3979807

Strand: Reverse

Name: pitB [H]

Synonym: ECH74115_4299

Alternate gene names: 209399859

Gene position: 3979807-3978491 (Counterclockwise)

Preceding gene: 209400603

Following gene: 209396093

Centisome position: 71.42

GC content: 51.94

Gene sequence:

>1317_bases
ATGCTAAATTTATTTGTTGGCCTTGATATATACACAGGGCTTTTGTTATTACTTGCTCTGGCATTTGTGTTGTTCTACGA
AGCAATCAATGGTTTTCATGACACGGCGAATGCGGTGGCAACCGTTATTTATACTCGTGCCATGCAGCCACAGCTTGCCG
TGGTGATGGCGGCATTTTTTAACTTTTTTGGCGTGTTATTGGGCGGACTTAGCGTTGCCTATGCCATTGTCCATATGTTG
CCAACCGATTTGTTGCTGAATATGGGGTCAACCCACGGCCTGGCGATGGTCTTTTCCATGCTGCTGGCGGCGATTATCTG
GAACCTGGGAACGTGGTTCTTTGGTTTACCGGCCTCCAGTTCGCATACCTTGATTGGCGCGATTATCGGCATCGGTTTAA
CCAACGCGCTGTTAACCGGCTCATCGGTGATGGATGCGTTAAACCTGCGTGAAGTGACCAAAATTTTCTCCTCGCTGATT
GTTTCCCCTATCGTCGGCCTGGTCATTGCGGGAGGCCTGATATTCCTGCTGCGACGCTACTGGAGCGGAACGAAAAAGCG
TGACCGTATTCACCGCATTCCGGAAGATCGCAAAAAGAAAAAAGGCAAACGTAAGCCGCCATTCTGGACGCGTATTGCGC
TGATTGTTTCCGCTGCGGGCGTGGCGTTTTCGCACGGCGCGAACGACGGACAAAAAGGGATCGGCCTGGTGATGCTGGTA
CTGGTGGGGATTGCCCCTGCTGGCTTCGTCGTCAATATGAACGCGTCCGGCTATGAAATTACCCGTACCCGCGATGCCGT
TACCAACTTCGAACACTACCTGCAACAGCATCCTGAACTGCCGCAGAAGTTGATTGCGATGGAACCTCCATTGCCTGCGG
CATCTACTGATGGCGCGCAAGTGACGGAGTTTCACTGTCATCCGGGAAATACCTTTGATGCGATTGCGCGCGTTAAAACG
ATGCTGCCAGGCAATATGGAAAGTTACGAGCCGTTAAGCGTGAGTCAGCGCAGCCAGCTGCGCCGCATTATGCTGTGCAT
CTCTGATACTTCCGCGAAGCTGGCGAAACTGCCAGGCGTCAGTAAAGAAGACCAGAACCTGCTGAAAAAACTGCGCAGCG
ATATGTTAAGCACCATTGAGTACGCTCCGGTGTGGATCATCATGGCAGTAGCACTGGCGCTCGGCATTGGCACCATGATT
GGCTGGCGTCGTGTTGCGATGACCATCGGTGAGAAGCCTTTTTTAATATCGTATTGTGTTCCTCCAGGCGGCGAACCTGC
TTTTCCAGTTCGCGGATACGTTGCTGGTCTGGAGTAA

Upstream 100 bases:

>100_bases
ACTCTTTTTATCGTTAAAAAATGATATTTCACTTTGCCCACACCGTTAAAGTCACTGATAATGCGTCCGTTCGTAAATTC
AAAATGGCGTAATCTAATAT

Downstream 100 bases:

>100_bases
TAGGTGTGGCAGAGGGCGCAATCCCCTGACGCTCTCGCCTGAGCTGGCGCACCCAACTCTCAAGCGTGGTAGAACCGACA
TTCATCGCTTCACTGGCTTG

Product: probable low-affinity inorganic phosphate transporter 2

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKT
MLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMI
GWRRVAMTIGEKPFLISYCVPPGGEPAFPVRGYVAGLE

Sequences:

>Translated_438_residues
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKT
MLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMI
GWRRVAMTIGEKPFLISYCVPPGGEPAFPVRGYVAGLE
>Mature_438_residues
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKT
MLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMI
GWRRVAMTIGEKPFLISYCVPPGGEPAFPVRGYVAGLE

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=169, Percent_Identity=30.1775147928994, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789360, Length=412, Percent_Identity=99.2718446601942, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI1789907, Length=412, Percent_Identity=79.126213592233, Blast_Score=638, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 47540; Mature: 47540

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFF
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
SHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
HCCCCHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGAQ
HHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCC
VTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGV
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
SKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAMTIGEKPFLISYCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PPGGEPAFPVRGYVAGLE
CCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFF
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
SHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
HCCCCHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGAQ
HHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCC
VTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGV
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
SKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAMTIGEKPFLISYCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PPGGEPAFPVRGYVAGLE
CCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 7775463 [H]