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The map label for this gene is gsp [H]

Identifier: 209400603

GI number: 209400603

Start: 3980099

End: 3981958

Strand: Reverse

Name: gsp [H]

Synonym: ECH74115_4300

Alternate gene names: 209400603

Gene position: 3981958-3980099 (Counterclockwise)

Preceding gene: 209399055

Following gene: 209399859

Centisome position: 71.46

GC content: 52.47

Gene sequence:

>1860_bases
ATGAGCAAAGGAACGACCAGCCAGGATGCCCCGTTCGGGACATTATTGGGCTACGCCCCAGGTGGGGTAGCAATCTACTC
TTCAGATTACAGTTCTCTCGATCCGCAGGAATACGAAGATGACGCCGTATTCCGTAGCTATATCGACGACGAATATATGG
GCCACAAATGGCAATGCGTTGAATTTGCTCGCCGTTTTCTCTTTCTGAACTACGGTGTGGTCTTTACTGACGTGGGTATG
GCGTGGGAGATTTTCTCGCTGCGCTTCCTGCGTGAAGTGGTTAATGACAACATCCTGCCATTGCAGGCATTTCCTAACGG
CTCGCCGCGTGCGCCGGTCGCGGGTGCGCTTCTTATCTGGGATAAAGGCGGTGAATTTAAAGACACTGGCCATGTCGCCA
TCATTACCCAATTACATGGCAACAAAGTCCGTATTGCGGAACAGAACGTGATTCATTCCCCGTTGCCGCAAGGGCAACAG
TGGACGCGCGAACTGGAGATGGTGGTCGAAAACGGCTGCTATACCCTTAAAGACACTTTTGATGACACCACCATTCTGGG
CTGGATGATCCAGACGGAAGATACTGAATACAGCTTACCGCAGCCGGAAATTGCAGGCGAGCTGCTGAAAATCAGCGGAG
CGCGTCTGGAAAACAAAGGCCAGTTTGACGGTAAATGGCTGGATGAAAAAGATCCGCTGCAAAACGCCTATGTGCAGGCC
AACGGTCAGGTGATCAATCAGGATCCGTATCATTACTACACCATTACCGAGAGCGCCGAGCAGGAGCTGATTAAAGCCAC
CAACGAGCTGCACCTGATGTATCTTCACGCAACCGACAAGGTGCTGAAAGATGACAACCTGCTGGCGCTGTTCGACATTC
CGAAAATCCTCTGGCCACGTTTGCGTCTCTCCTGGCAGCGTCGCCGTCACCATATGATCACCGGTCGTATGGATTTCTGC
ATGGATGAGCGTGGTCTGAAGGTTTACGAGTACAACGCCGACTCCGCCTCCTGTCATACCGAAGCGGGCTTGATCCTCGA
ACGTTGGGCGGAGCAGGGCTATAAAGGCAACGGCTTCAATCCGGCGGAAGGGCTGATTAACGAACTGGCTGGTGCCTGGA
AACACAGTCGTGCACGTCCGTTTGTCCATATCATGCAGGACAAAGATATCGAGGAAAACTATCACGCGCAGTTTATGGAG
CAGGCGCTGCACCAGGCGGGCTTTGAAACGCGTATCTTGCGCGGGCTGGATGAACTGGGCTGGGATGCTGCCGGGCAACT
GATTGATGGGGAAGGGCGACTGGTTAACTGCGTGTGGAAAACCTGGGCGTGGGAAACCGCGTTTGATCAGATTCGTGAAG
TTAGCGACCGTGAGTTTGCTGCGGTGCCGATCCGTACCGGTCATCCGCAAAATGAAGTGCGTCTTATCGACGTATTGCTG
CGCCCGGAAGTGCTGGTCTTTGAGCCGCTGTGGACGGTGATCCCCGGCAACAAAGCGATTCTGCCGATCCTCTGGTCGCT
GTTCCCGCACCATCGTTACCTGCTGGATACCGATTTCACTGTTAATGATGAATTGGTGAAAACAGGTTACGCAGTGAAAC
CGATCGCCGGTCGCTGTGGCAGCAACATCGACCTCGTCAGCCATCATGAAGAGGTGCTGGACAAAACCAGTGGTAAATTT
GCCGAGCAGAAAAACATCTATCAGCAACTGTGGTGTTTGCCGAAAGTGGACGGTAAATACATTCAGGTATGTACCTTCAC
CGTTGGCGGCAACTACGGTGGGACGTGTTTGCGCGGTGATGAATCACTGGTTATTAAAAAAGAGAGTGATATTGAACCGT
TAATTGTGGTGAAAGAGTAA

Upstream 100 bases:

>100_bases
TATGATTAGTCTGACCTCTGTCCGGTTAATGTCGCTGAGCAGGTAATCAGAGCACAACAATTACGCCAACTGCTTAGGTT
GTATTGTTAAAGGTAAAGTG

Downstream 100 bases:

>100_bases
TACATGTTGTAGCAATCCCGTACTGATTATGAAGAATAGTCAGTACGGGGATTAATCAAAATATTCAATATTTATATGTT
CAAATATTTATTCATTTCAT

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFME
QALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKF
AEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE

Sequences:

>Translated_619_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC
MDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFME
QALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKF
AEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE
>Mature_618_residues
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMA
WEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQW
TRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQAN
GQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQ
ALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFA
EQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=619, Percent_Identity=99.8384491114701, Blast_Score=1287, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=391, Percent_Identity=27.3657289002558, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1790629, Length=374, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 70534; Mature: 70402

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCV
CCCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEE
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTF
CCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCC
DDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
CCEEECCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC
PAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELG
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
WDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
CCCCCCEECCCCCEEEEEEHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCEECCCHHCCC
SNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECC
ESLVIKKESDIEPLIVVKE
CEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCV
CCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEE
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTF
CCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCC
DDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
CCEEECCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHH
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFN
HHHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC
PAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELG
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
WDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
CCCCCCEECCCCCEEEEEEHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCEECCCHHCCC
SNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECC
ESLVIKKESDIEPLIVVKE
CEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]