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The map label for this gene is torA

Identifier: 209399846

GI number: 209399846

Start: 1242642

End: 1245188

Strand: Direct

Name: torA

Synonym: ECH74115_1233

Alternate gene names: 209399846

Gene position: 1242642-1245188 (Clockwise)

Preceding gene: 209400451

Following gene: 209399417

Centisome position: 22.3

GC content: 55.2

Gene sequence:

>2547_bases
ATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCT
GGGGCCGTCATTGTTAACGTCGCGCCGTGCGACTGCGGCGCAAGCGGCGACTGAGGCTGTCATCTCGAAAGAGGGCATTC
TTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGAACTGGAT
AAATATCCGTCGAAAATGATTGCCGGATTGCCGGATCATGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGT
GGACTGGCTGCGTAAGCGCCATCTGAGCGACACCTCCCAGCGCGGTGATAACCGTTTTGTGCGTGTGAGCTGGGATGAAG
CCCTCGACATGTTCTATGAAGAACTGGAACGCGTACAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGG
CAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGG
CGGAGATTACTCTACTGGTGCTGCGCAGGTGATCCTGCCGCGCGTAGTCGGTTCGATGGAAGTGTATGAACAGCAAACCT
CCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGAAAAACCAGCAAGCGAACTGG
TGGTGCCCGGATCACGATGTTTATGAATATTACGCGCAGTTGAAAGCGAAAGTCGCCGCCGGTGAAATTGAGGTCATTAG
CATCGATCCGGTTGTTACATCCACCCATGAGTATCTGGGGCGCGAGCATGTGAAGCACATTGCGGTTAACCCGCAAACTG
ACGTGCCGCTGCAACTGGCGCTGGCGCATACGTTGTACAGTGAAAACCTGTACGACAAAAACTTCCTCGCTAACTACTGT
GTGGGTTTTGAGCAGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACT
GACCGGCATTGATGCCGAAACCATTCGTGGACTGGCGCGGCAGATGGCGGCGAACAGAACGCAGATTATTGCTGGCTGGT
GCGTACAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCTG
CCAGGTGGTGGCTTTGGTTTTGGCTGGCACTATAACGGCGCAGGCACGCCGGGGCGTAAAGGCGTTATTCTGAGTGGTTT
CTCCGGCTCTACGTCGATTCCGCCTGTTCACGACAACAGTGATTACAAAGGCTACAGCAGCACCATTCCGATTGCCCGTT
TTATCGATGCGATCCTCGAACCGGGGAAAGTAATCAACTGGAACGGTAAATCGGTAAAACTGCCGCCGCTGAAAATGTGT
ATTTTTGCCGGAACTAACCCCTTCCATCGCCATCAGCAGATCAACCGCATTATTGAAGGCTGGCGCAAACTGGAAACGGT
TATCGCCATAGATAACCAGTGGACCTCAACCTGCCGCTTTGCCGATATCGTGCTGCCTGCGACCACGCAGTTTGAGCGTA
ACGATCTCGACCAGTACGGCAACCACTCCAACCGTGGCATTATCGCCATGAAACAGGTCGTGCCGCCGCAGTTCGAGGCG
CGCAACGACTTTGATATTTTCCGCGAGCTGTGCCGCCGCTTTAATCGCGAAGAAGCCTTTACCGAAGGGCTGGACGAAAT
GGGCTGGCTGAAACGCATCTGGCAGGAAGGTGTTCAGCAAGGCAAAGGACGCGGCGTTCATTTGCCAGCGTTTGATGACT
TCTGGAATAACAAAGAGTACGTCGAGTTTGACCATCCGCAGATGTTTGTTCGCCACCAGGCATTCCGCGAAGATCCAGAT
CTCGAACCGCTGGGCACGCCGAGTGGCCTGATTGAGATCTATTCGAAAACCATCGCCGATATGAACTACGACGATTGTCA
GGGGCATCCGATGTGGTTTGAGAAAATCGAACGCTCCCACGGTGGGCCCGGCTCGCAGACGTATCCGTTGCATCTGCAAT
CTGTGCATCCGGATTTCCGACTTCACTCGCAGTTATGTGAGTCGGAAACTCTGCGTCAGCAATATACGGTAGCGGGTAAA
GAGCCAGTGTTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCG
CGGTCAGGTGTTGGCAGGGGCAGTAGTTTCTGACCGCTATGCACCCGGCGTGGCGCGAATTCACGAAGGGGCATGGCACG
ATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATTGACATCGGTACTTCG
CAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTGCAACGGAACAGTGGAGCAGGTAACGGC
GTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATTATGA

Upstream 100 bases:

>100_bases
GCCTCGATAAACGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGC
GATAAGAAGGAAGAAAAATA

Downstream 100 bases:

>100_bases
CCACGCTGACAGCACAACAGATAGCCTGTGTTTACGCCTGGCTGGCGCAGTTGTTCTCCCGTGAACTGGACGATGAACAA
CTGACGCAAATCGCCAGTGC

Product: trimethylamine-N-oxide reductase

Products: NA

Alternate protein names: TMAO reductase 1; Trimethylamine oxidase 1

Number of amino acids: Translated: 848; Mature: 848

Protein sequence:

>848_residues
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAATEAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELD
KYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANW
WCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYC
VGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGL
PGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMC
IFAGTNPFHRHQQINRIIEGWRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEA
RNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPD
LEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGK
EPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIGTS
QLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL

Sequences:

>Translated_848_residues
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAATEAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELD
KYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANW
WCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYC
VGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGL
PGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMC
IFAGTNPFHRHQQINRIIEGWRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEA
RNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPD
LEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGK
EPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIGTS
QLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL
>Mature_848_residues
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAATEAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELD
KYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANW
WCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYC
VGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGL
PGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMC
IFAGTNPFHRHQQINRIIEGWRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEA
RNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPD
LEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGK
EPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIGTS
QLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL

Specific function: Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family

Homologues:

Organism=Escherichia coli, GI1787231, Length=847, Percent_Identity=99.2916174734357, Blast_Score=1755, Evalue=0.0,
Organism=Escherichia coli, GI87081994, Length=785, Percent_Identity=45.2229299363057, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=790, Percent_Identity=42.6582278481013, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI87081797, Length=761, Percent_Identity=31.9316688567674, Blast_Score=300, Evalue=4e-82,
Organism=Escherichia coli, GI1787870, Length=864, Percent_Identity=29.2824074074074, Blast_Score=277, Evalue=2e-75,
Organism=Escherichia coli, GI171474008, Length=854, Percent_Identity=29.3911007025761, Blast_Score=259, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TORA_ECO57 (P58360)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85635
- PIR:   H90772
- RefSeq:   NP_286933.1
- RefSeq:   NP_309179.1
- ProteinModelPortal:   P58360
- SMR:   P58360
- EnsemblBacteria:   EBESCT00000028320
- EnsemblBacteria:   EBESCT00000055968
- GeneID:   913030
- GeneID:   959060
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1415
- KEGG:   ecs:ECs1152
- GeneTree:   EBGT00050000008999
- HOGENOM:   HBG304064
- OMA:   QQYAVGG
- ProtClustDB:   PRK15102
- BioCyc:   ECOL83334:ECS1152-MONOMER
- InterPro:   IPR009010
- InterPro:   IPR006658
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006655
- InterPro:   IPR006311
- InterPro:   IPR011887
- Gene3D:   G3DSA:2.40.40.20
- TIGRFAMs:   TIGR00509
- TIGRFAMs:   TIGR01409
- TIGRFAMs:   TIGR02164

Pfam domain/function: PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.2.3

Molecular weight: Translated: 94447; Mature: 94447

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAATEAVISKEGILTGSHWG
CCCCHHHHHHHHHHHHHHCCCEEEHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
AIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQ
EEEEEECCCEEEEECCCCCCCCHHHHHHCCCHHHCCHHHHCCCHHHHHHHHHHHCCCHHH
RGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGWQSTGMFHNASGMLAKAIALH
CCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCHHHHHHHHC
GNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECHHHHCCCCCCC
WCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLA
CCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCHHHH
LAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLAR
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
QMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGLPGGGFGFGWHYNGAGTPGRK
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
GVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMC
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCEEECCCEEEE
IFAGTNPFHRHQQINRIIEGWRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYG
EEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHC
NHSNRGIIAMKQVVPPQFEARNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQ
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHH
GKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIAD
CCCCEEECCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
MNYDDCQGHPMWFEKIERSHGGPGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGK
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
EPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGE
CCEEECCCCCCCCCCCCCCEEEEECCCCCEEECHHHHCCCCCCHHHHCCCCCCCCCCCCC
PGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEY
CCCEEECCCCCEEEEECCHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHCCC
VPASQVKL
CCHHCCCC
>Mature Secondary Structure
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAATEAVISKEGILTGSHWG
CCCCHHHHHHHHHHHHHHCCCEEEHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
AIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQ
EEEEEECCCEEEEECCCCCCCCHHHHHHCCCHHHCCHHHHCCCHHHHHHHHHHHCCCHHH
RGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGWQSTGMFHNASGMLAKAIALH
CCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCHHHHHHHHC
GNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECHHHHCCCCCCC
WCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLA
CCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCHHHH
LAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLAR
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
QMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGLPGGGFGFGWHYNGAGTPGRK
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
GVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMC
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCEEECCCEEEE
IFAGTNPFHRHQQINRIIEGWRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYG
EEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHC
NHSNRGIIAMKQVVPPQFEARNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQ
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHH
GKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIAD
CCCCEEECCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
MNYDDCQGHPMWFEKIERSHGGPGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGK
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
EPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGE
CCEEECCCCCCCCCCCCCCEEEEECCCCCEEECHHHHCCCCCCHHHHCCCCCCCCCCCCC
PGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEY
CCCEEECCCCCEEEEECCHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHCCC
VPASQVKL
CCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796