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The map label for this gene is mdtO

Identifier: 209399505

GI number: 209399505

Start: 5228913

End: 5230964

Strand: Reverse

Name: mdtO

Synonym: ECH74115_5589

Alternate gene names: 209399505

Gene position: 5230964-5228913 (Counterclockwise)

Preceding gene: 209400246

Following gene: 209399819

Centisome position: 93.88

GC content: 54.48

Gene sequence:

>2052_bases
ATGAGCGCGCTCAACTCCCTGCCATTACCGGTGGTCAGGCTGCTGGCGTTCTTTCATGAAGAGTTAAGCGAGCGGCGACC
AGGTCGCGTGCCGCAGACCGTGCAACTCTGGGTAGGCTGCCTGCTGGTGATTCTGATCTCGATGACTTTTGAGATCCCTT
TTGTGGCGTTATCGCTGGCAGTGCTGTTTTACGGTATTCAGTCGAACGCGTTTTACACCAAATTTGTCGCGATCTTGTTT
GTGGTTGCCACGGTGCTGGAGATCGGCAGCCTGTTTTTGATCTACAAATGGTCATACGGCGAACCGTTGATCCGCTTGAT
CATCGCCGGGCCGATCCTGATGGGCTGCATGTTTTTGATGCGCACCCATCGGTTGGGACTGGTCTTTTTCGCTGTCGCCA
TTGTCGCCATTTATGGGCAAACCTTCCCCGCCATGCTCGACTATCCGGAAGTGGTCGTGCGCTTAACGCTGTGGTGTATC
GTTGTTGGCCTCTACCCGACGTTATTAATGACGTTAATCGGCGTACTGTGGTTTCCCAGTCGTGCCATTTCGCAAATGCA
TCAAGCGCTTAATGATCGGCTTGATGATGCCATTAGCCACCTGACGGACAGCCTCGCACCGCTACCCGAAACGCGGATTG
AAAGAGAGGCGCTGGCGCTGCAAAAACTCAATGTCTTTTGCCTCGCGGACGATGCCAACTGGCGAACTCAAAGCGCATGG
TGGCAAAGCTGCGTGGCAACGGTAACCTACATTTACTCGACGCTGAATCGCTACGATCCCACCTCTTTTGCTGATTCTCA
GGCAATTATTGAATTCCGACAAAAATTAGCTTCAGAAATCAACAAGCTGCAGCATGCCATTACCGAAGGTCAGTGCTGGC
AAAGCGACTGGCGGATCAGTGAAAGTGAAGCGATGGCGGCACGGGAATGTAACCTGGAGAATATCTGCCAGACGTTGTTA
CAACTGGGTCAGATGGACCCGAATACGCCGCCAACGCCCGCCGCCAAACCGCCATCAATGGTCGCTGATGCTTTTACCAA
TCCAGACTATATGCGCTACGCGGTAAAAACGCTGCTCGCCTGTTTGATCTGTTACACCTTCTACAGCGGCGTGGACTGGG
AAGGCATTCACACCTGTATGCTGACCTGCGTGATCGTCGCTAATCCGAATGTCGGTTCATCGTACCAGAAGATGGTGCTG
CGTTTTGGCGGGGCCTTTTGCGGCGCGATTCTGGCGCTGTTATTCACGCTACTGGTCATGCCCTGGCTGGACAATATTGT
CGAATTGCTGTTTGTGCTGGCACCGATTTTCCTGTTGGGCGCATGGATTGCCACCAGCTCTGAACGCTCTTCTTATATCG
GCACACAGATGGTGGTCACCTTCGCGCTCGCCACGCTCGAAAACGTTTTTGGTCCGGTGTACGACCTGGTGGAAATTCGC
GATCGCGCCCTGGGTATCATCATTGGTACCGTGGTGTCTGCGGTGATTTACACCTTTGTCTGGCCTGAAAGTGAAGCGCG
CACGCTGCCGCAAAAACTGGCTGGCGCGCTGGGTATGCTAAGTAAAGTAATGCGGATCCCACGCCAGCAGGAAGTCACGG
CTCTGCGCACTTATCTGCAAATTCGTATAGGTCTGCATGCGGCGTTTAATGCCTGTGAAGAGATGTGCCAACGCGTGGCG
CTGGAGCGTCAACTGGACAGCGAAGAACGCGCCTTACTGATTGAACGTTCGCAAACGGTTATTCATCAGGGCCGCGATCT
TCTTCACGCCTGGGATGCCACCTGGAACTCGGCGCAGGCGCTGGATAACGCACTACAGCCGGACAAAGCAGGTCAGTTTG
CCGACGCCCTGGAAAAATACGCTGCCGGTCTGGCAACCGCACTCAGCCGTTCTCCTCAAATAACGCTTGAAGAGACACCC
GCCTCGCAGGCCATCCTGCCCACCTTATTAAAACAGGAGCAACACGTCTGCCAGCTTTTCGCCCGCTTGCCAGACTGGAC
AGCCCCGGCATTAACGCCCGCCACGGAACAGGCACAAGGAGCCACGCAATGA

Upstream 100 bases:

>100_bases
GTGTTGCCCAGCGTTTTCCGGTCAAAATCATGGTTGATAAACCTGACCCGGAAATGTTCCGCATCGGCGCTTCGGCAGTC
GCTAATCTTGAGCCGCAATA

Downstream 100 bases:

>100_bases
TCAATCGTCAACTTTCACGTCTGCTGTTGTGCAGCATTCTCGGCAGCACGACGCTGATTTCCGGCTGTGCCCTGGTACGC
AAGGATTCTGCGCCTCATCA

Product: multidrug efflux system protein MdtO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILF
VVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQKLNVFCLADDANWRTQSAW
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLL
QLGQMDPNTPPTPAAKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVL
RFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVA
LERQLDSEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGLATALSRSPQITLEETP
ASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ

Sequences:

>Translated_683_residues
MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILF
VVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQKLNVFCLADDANWRTQSAW
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLL
QLGQMDPNTPPTPAAKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVL
RFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVA
LERQLDSEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGLATALSRSPQITLEETP
ASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
>Mature_682_residues
SALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFV
VATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCIV
VGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQKLNVFCLADDANWRTQSAWW
QSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQ
LGQMDPNTPPTPAAKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLR
FGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIRD
RALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVAL
ERQLDSEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGLATALSRSPQITLEETPA
SQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride

COG id: COG1289

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MdtO family

Homologues:

Organism=Escherichia coli, GI87082367, Length=683, Percent_Identity=98.9751098096633, Blast_Score=1387, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDTO_ECO57 (Q8X5R8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C86102
- PIR:   G91261
- RefSeq:   NP_290714.2
- RefSeq:   NP_313090.2
- EnsemblBacteria:   EBESCT00000025265
- EnsemblBacteria:   EBESCT00000059985
- GeneID:   914278
- GeneID:   960043
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5681
- KEGG:   ecs:ECs5063
- GeneTree:   EBGT00050000011297
- HOGENOM:   HBG467485
- OMA:   DWRITES
- ProtClustDB:   PRK11427
- BioCyc:   ECOL83334:ECS5063-MONOMER
- GO:   GO:0006810
- InterPro:   IPR006726

Pfam domain/function: PF04632 FUSC

EC number: NA

Molecular weight: Translated: 76120; Mature: 75988

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13099df0)-; HASH(0x137c4c40)-; HASH(0x1393310c)-; HASH(0x133061cc)-; HASH(0x1311dd44)-; HASH(0x11e707a0)-; HASH(0x1393003c)-; HASH(0x13935920)-; HASH(0x13669138)-;

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCIVVGLYPTLLMTLIGVLWFPS
HHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQKLNVFCLADDANWRTQSAW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAITEGQCWQSDWRIS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESEAMAARECNLENICQTLLQLGQMDPNTPPTPAAKPPSMVADAFTNPDYMRYAVKTLLA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
CLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLRFGGAFCGAILALLFTLLVM
HHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRIGLHAAFNACEEMCQRVALERQLDSEERALLIERSQTVIHQGRDLLHAWDATWNSAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
LDNALQPDKAGQFADALEKYAAGLATALSRSPQITLEETPASQAILPTLLKQEQHVCQLF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
ARLPDWTAPALTPATEQAQGATQ
HHCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
SALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCIVVGLYPTLLMTLIGVLWFPS
HHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQKLNVFCLADDANWRTQSAW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAITEGQCWQSDWRIS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESEAMAARECNLENICQTLLQLGQMDPNTPPTPAAKPPSMVADAFTNPDYMRYAVKTLLA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
CLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLRFGGAFCGAILALLFTLLVM
HHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRIGLHAAFNACEEMCQRVALERQLDSEERALLIERSQTVIHQGRDLLHAWDATWNSAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
LDNALQPDKAGQFADALEKYAAGLATALSRSPQITLEETPASQAILPTLLKQEQHVCQLF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
ARLPDWTAPALTPATEQAQGATQ
HHCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796