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The map label for this gene is mdtN

Identifier: 209400246

GI number: 209400246

Start: 5230964

End: 5231995

Strand: Reverse

Name: mdtN

Synonym: ECH74115_5590

Alternate gene names: 209400246

Gene position: 5231995-5230964 (Counterclockwise)

Preceding gene: 209400980

Following gene: 209399505

Centisome position: 93.9

GC content: 55.23

Gene sequence:

>1032_bases
ATGGAAAGTACGCCGAAAAAAGCTCCTCGCAGTAAATTCCCTGCTCTGTTAGTGGTTGCGTTGGCGCTGGTTGCCCTTGT
TTTCGTTATCTGGCGCGTAGACAGTGCGCCATCAACTAATGACGCTTACGTGTCAGCAGATACCATTGATGTGGTGCCGG
AAGTCAGCGGCCGCATTGTAGAACTGGCGGTCACCGACAACCAGGCAGTCAAACAGGGCGATTTGCTGTTCCGCATTGAC
CCGCGCCCGTACGAAGCCAATCTGGCGAAATCTGAAGCCTCCCTCGCGGCACTGGATAAGCAAATTATGCTCACCCAGCG
TAGCGTTGACGCGCAACAGTTTGGTGCCGACTCGGTTAACGCCACGGTAGAAAAAGCCCGTGCCGCCGCTAAACAGGCCA
CAGATACATTACGCCGCACCGAGCCATTACTGAAAGAAGGTTTTGTCTCAGCGGAAGACGTTGACCGTGCGAGGACCGCG
CAGCGCGCTGCAGAAGCCGATCTTAATGCTGTATTGTTGCAGGCGCAGTCAGCCGCCAGCGCCGTCAGCGGCGTGGATGC
ATTAGTTGCCCAGCGTGCGGCGGTCGAAGCGGATATTGCCCTGACCAAACTGCATCTGGAAATGGCGACCGTTCGCGCAC
CGTTTGATGGCCGGGTCATTTCCCTCAAAACCTCCGTCGGGCAATTTGCTTCTGCCATGCGCCCTATTTTTACCCTAATC
GACACTCGTCACTGGTACGTGATCGCCAACTTCCGCGAAACCGATCTGAAAAATATTCGCTCAGGTACACCTGCAACGAT
TCGCCTGATGAGTGACAGCGGCAAAACTTTCGAGGGTAAAGTGGATTCAATTGGCTACGGCGTGCTACCGGATGACGGCG
GCCTGGTGCTGGGCGGCCTGCCGAAAGTTTCTCGTTCTATTAACTGGGTCCGTGTTGCCCAGCGTTTTCCGGTCAAAATC
ATGGTTGATAAACCTGACCCGGAAATGTTCCGCATCGGCGCTTCGGCAGTCGCTAATCTTGAGCCGCAATAA

Upstream 100 bases:

>100_bases
TATCAAGCTGGCATTTGTCGGAATTATTTATACCGCCCTTTTTGCTCTCTACGCCATGCTGTTCTGGCTGGCATTTTTCT
AATCTATTTGAGACGCGCTC

Downstream 100 bases:

>100_bases
TGAGCGCGCTCAACTCCCTGCCATTACCGGTGGTCAGGCTGCTGGCGTTCTTTCATGAAGAGTTAAGCGAGCGGCGACCA
GGTCGCGTGCCGCAGACCGT

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYVSADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKSEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVDKPDPEMFRIGASAVANLEPQ

Sequences:

>Translated_343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYVSADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKSEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVDKPDPEMFRIGASAVANLEPQ
>Mature_343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYVSADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKSEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVDKPDPEMFRIGASAVANLEPQ

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI1790519, Length=343, Percent_Identity=99.4169096209913, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI87081951, Length=318, Percent_Identity=32.7044025157233, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1789637, Length=337, Percent_Identity=28.7833827893175, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788711, Length=352, Percent_Identity=27.8409090909091, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789041, Length=333, Percent_Identity=29.1291291291291, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790012, Length=258, Percent_Identity=29.4573643410853, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790024, Length=254, Percent_Identity=27.1653543307087, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDTN_ECO57 (Q8X5R2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D86102
- PIR:   H91261
- RefSeq:   NP_290715.1
- RefSeq:   NP_313091.1
- ProteinModelPortal:   Q8X5R2
- SMR:   Q8X5R2
- EnsemblBacteria:   EBESCT00000024364
- EnsemblBacteria:   EBESCT00000058837
- GeneID:   914275
- GeneID:   960042
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5682
- KEGG:   ecs:ECs5064
- GeneTree:   EBGT00050000009283
- HOGENOM:   HBG624965
- OMA:   GQFASTM
- ProtClustDB:   PRK10476
- BioCyc:   ECOL83334:ECS5064-MONOMER
- InterPro:   IPR005694
- InterPro:   IPR006143
- TIGRFAMs:   TIGR00998

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 36953; Mature: 36953

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12fc4c20)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYVSADTIDVVPEVSGRIV
CCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEECCCCCCCEE
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQIMLTQRSVDAQQFGADSVN
EEEECCCCHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEHHHHHH
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHH
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
CCCEEEEEEECCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEECC
PKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ
HHHHHCCHHEEHHHHCCEEEEEECCCCHHEEECHHHHCCCCCH
>Mature Secondary Structure
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYVSADTIDVVPEVSGRIV
CCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEECCCCCCCEE
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQIMLTQRSVDAQQFGADSVN
EEEECCCCHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEHHHHHH
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHH
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
CCCEEEEEEECCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEECC
PKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ
HHHHHCCHHEEHHHHCCEEEEEECCCCHHEEECHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796