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The map label for this gene is engD

Identifier: 209398838

GI number: 209398838

Start: 1624099

End: 1625190

Strand: Reverse

Name: engD

Synonym: ECH74115_1684

Alternate gene names: 209398838

Gene position: 1625190-1624099 (Counterclockwise)

Preceding gene: 209398573

Following gene: 209396595

Centisome position: 29.17

GC content: 51.1

Gene sequence:

>1092_bases
ATGGGATTCAAATGCGGTATCGTCGGTTTGCCCAACGTCGGGAAATCTACCCTGTTCAACGCGCTGACCAAAGCCGGTAT
TGAAGCGGCCAACTTTCCATTCTGCACCATTGAGCCGAACACAGGCGTCGTACCAATGCCTGATCCTCGCCTGGATCAAC
TGGCTGAAATCGTAAAACCGCAACGTACGCTTCCCACGACCATGGAATTTGTCGATATCGCCGGTCTGGTAAAAGGCGCA
TCGAAAGGCGAAGGTCTGGGTAACCAGTTCCTGACCAACATCCGTGAAACCGAAGCGATCGGTCACGTTGTTCGCTGCTT
TGAAAATGACAACATCATTCACGTTTCCGGCAAAGTTAACCCGGCTGACGATATTGAAGTTATCAACACCGAACTGGCGC
TGGCAGACCTCGACACCTGCGAACGTGCGATTCATCGCGTACAGAAGAAAGCCAAAGGTGGCGATAAAGACGCGAAAGCT
GAGCTGGCGGTCCTGGAAAAATGCCTGCCCCAGCTGGAAAACGCAGGTATGCTGCGCGCGCTGGATTTAAGCGCTGAAGA
GAAAGCGGCTATTCGTTACCTGAGCTTCCTGACGCTGAAACCAACAATGTACATCGCCAACGTCAACGAAGACGGTTTTG
AAAACAACCCATATCTTGACCAGGTGCGTGAAATCGCGGCGAAAGAAGGTTCTGTTGTGGTTCCGGTTTGTGCTGCTGTT
GAAGCAGACATTGCCGAACTGGACGACGAAGAACGTGACGAGTTTATGCAGGAGCTTGGGCTGGAAGAGCCGGGTCTGAA
CCGTGTGATCCGTGCCGGTTATAAGCTGCTGAACCTGCAAACTTACTTCACCGCTGGGGTGAAAGAAGTGCGTGCATGGA
CCATTCCGGTTGGAGCAACCGCGCCGCAGGCAGCGGGCAAAATCCATACTGATTTTGAAAAAGGCTTTATCCGTGCACAA
ACCATCTCGTTTGAAGATTTCATCACTTACAAAGGTGAACAAGGCGCGAAAGAAGCAGGCAAAATGCGTGCAGAAGGTAA
AGATTACATCGTGAAAGATGGCGATGTGATGAACTTCCTTTTCAACGTCTAA

Upstream 100 bases:

>100_bases
TTTTTGCCGAGTGCCGTGTATAATAGGCAAAGTTATTTCCATTTCTGCAATCTGTTAGCAATAACAGGTTGATTATTAAG
ATATTAAGGTGATTTAAATC

Downstream 100 bases:

>100_bases
TTAATTTATTGTCTCATGAGGTTTCGCTAAATCTCATGAAGAGCGCAAAAGTATATAAAATCCACGCAATTGCGTGGATT
TTTCATTTCACCGAGTCCTA

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGA
SKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAV
EADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQ
TISFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNFLFNV

Sequences:

>Translated_363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGA
SKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAV
EADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQ
TISFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNFLFNV
>Mature_362_residues
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGAS
KGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKAE
LAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAVE
ADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQT
ISFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=44.7154471544715, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI58761502, Length=227, Percent_Identity=39.647577092511, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=100, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=365, Percent_Identity=41.3698630136986, Blast_Score=289, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6319499, Length=368, Percent_Identity=39.6739130434783, Blast_Score=262, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=34.6456692913386, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321649, Length=145, Percent_Identity=37.9310344827586, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=44.2622950819672, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=44.2622950819672, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=44.2622950819672, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=42.0731707317073, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ENGD_ECO57 (P0ABU3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90842
- RefSeq:   NP_287449.1
- RefSeq:   NP_309735.1
- ProteinModelPortal:   P0ABU3
- SMR:   P0ABU3
- EnsemblBacteria:   EBESCT00000027705
- EnsemblBacteria:   EBESCT00000056828
- GeneID:   913155
- GeneID:   960453
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1974
- KEGG:   ecs:ECs1708
- GeneTree:   EBGT00050000009733
- HOGENOM:   HBG586249
- OMA:   QAANFPF
- ProtClustDB:   PRK09601
- BioCyc:   ECOL83334:ECS1708-MONOMER
- GO:   GO:0005622
- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192
- Gene3D:   G3DSA:3.10.20.30
- PANTHER:   PTHR23305:SF4
- PIRSF:   PIRSF006641
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00092

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C; SSF81271 TGS-like

EC number: NA

Molecular weight: Translated: 39668; Mature: 39537

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKP
CCEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
QRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSGKVN
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKAELAVLEKCLPQLENAGMLRA
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAV
ECCCCHHHHHHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHH
EADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGAT
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHEEEEEECCCC
APQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNFL
CCHHCCCCCCHHHHCEEEEEEECHHHHEEECCCCCHHHHHHHHCCCCCEEEECCHHHHHH
FNV
HCC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKP
CEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
QRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSGKVN
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKAELAVLEKCLPQLENAGMLRA
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAV
ECCCCHHHHHHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHH
EADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGAT
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHEEEEEECCCC
APQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNFL
CCHHCCCCCCHHHHCEEEEEEECHHHHEEECCCCCHHHHHHHHCCCCCEEEECCHHHHHH
FNV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796