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The map label for this gene is ybiX

Identifier: 209398451

GI number: 209398451

Start: 966335

End: 967021

Strand: Reverse

Name: ybiX

Synonym: ECH74115_0953

Alternate gene names: 209398451

Gene position: 967021-966335 (Counterclockwise)

Preceding gene: 209397104

Following gene: 209396378

Centisome position: 17.35

GC content: 51.97

Gene sequence:

>687_bases
ATGATGTACCACATTCCCGGCGTGTTATCGCCACAGGACGTCGCCCGTTTTCGCGAACAACTGGAACAAGCCGAATGGGT
GGATGGACGCGTCACCACCGGCGCACAAGGTGCGCAAGTTAAGAACAATCAACAGGTAGACACCCGCAGCACGTTATACG
CCGCCCTGCAAAATGAGGTGCTGAACGCGGTTAACCAACATGCTTTGTTCTTTGCCGCGGCCTTGCCGCGTACCCTTTCC
ACGCCGCTGTTTAATCGCTATCAGAACAATGAAACCTATGGTTTTCATGTGGATGGCGCAGTACGCAGCCATCCACAAAA
CGGCTGGATGCGCACTGACCTTTCTGCCACGCTGTTTTTAAGCGATCCACAAAGCTACGACGGCGGCGAACTGGTCGTTA
ATGACACCTTCGGACAACATCGGGTAAAACTCCCGGCAGGTGATCTGGTGTTGTATCCCTCCAGCAGCCTGCATTGCGTG
ACACCCGTAACCCGCGGCGTACGAGTGGCATCATTTATATGGATCCAGTCGATGATCCGCGATGATAAAAAGCGCGCCAT
GCTGTTTGAACTGGACAAAAATATTCAGAATATTCAGTCGCTGAAAAGCCGCTATGGTGAAAATGAAGAGATCCTGTCGC
TGCTCAATCTTTATCATAATCTGCTGCGGGAATGGTCGGAGATCTGA

Upstream 100 bases:

>100_bases
ACCGTTATCACCCGGGCGAGCCAAGAACCTTCTTGCTCACAGCCAATATGCATTTCTGATTCAGATGTGGGGCGCAGGCC
CCACTTTTTGGAGAACTTGT

Downstream 100 bases:

>100_bases
CGCTTGAACTGCCCAGGTTCGTCCCTATATTAGCTACACTTAACTATACAAGCATTGATATGGGGAGGACGATATGGCAT
CCGGTTGGGCTAACGATGAC

Product: putative hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQQVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLS
TPLFNRYQNNETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLYHNLLREWSEI

Sequences:

>Translated_228_residues
MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQQVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLS
TPLFNRYQNNETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLYHNLLREWSEI
>Mature_228_residues
MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQQVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLS
TPLFNRYQNNETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLYHNLLREWSEI

Specific function: Unknown

COG id: COG3128

COG function: function code S; Uncharacterized iron-regulated protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain

Homologues:

Organism=Escherichia coli, GI87081784, Length=228, Percent_Identity=97.3684210526316, Blast_Score=460, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBIX_ECO57 (Q8X7W8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90739
- PIR:   D85589
- RefSeq:   NP_286568.1
- RefSeq:   NP_308909.2
- ProteinModelPortal:   Q8X7W8
- SMR:   Q8X7W8
- EnsemblBacteria:   EBESCT00000027822
- EnsemblBacteria:   EBESCT00000057748
- GeneID:   917621
- GeneID:   958040
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1025
- KEGG:   ecs:ECs0882
- GeneTree:   EBGT00050000009218
- HOGENOM:   HBG285605
- OMA:   ADWADGK
- ProtClustDB:   PRK05467
- BioCyc:   ECOL83334:ECS0882-MONOMER
- HAMAP:   MF_00657
- InterPro:   IPR005123
- InterPro:   IPR006620
- SMART:   SM00702

Pfam domain/function: PF03171 2OG-FeII_Oxy

EC number: 1.14.11.- [C]

Molecular weight: Translated: 25908; Mature: 25908

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS51471 FE2OG_OXY

Important sites: BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQQVDTRSTLYAALQNEV
CEEECCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVRSHPQNGWMRTDLSATLFL
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCHHHCCCCCCCEEECCEEEEEE
SDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCVTPVTRGVRVASFIWIQSMIR
ECCCCCCCCEEEEECCCCCEEEECCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHH
DDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLYHNLLREWSEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQQVDTRSTLYAALQNEV
CEEECCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVRSHPQNGWMRTDLSATLFL
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCHHHCCCCCCCEEECCEEEEEE
SDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCVTPVTRGVRVASFIWIQSMIR
ECCCCCCCCEEEEECCCCCEEEECCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHH
DDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLYHNLLREWSEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796