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The map label for this gene is ldcA

Identifier: 209397773

GI number: 209397773

Start: 1620361

End: 1621275

Strand: Reverse

Name: ldcA

Synonym: ECH74115_1679

Alternate gene names: 209397773

Gene position: 1621275-1620361 (Counterclockwise)

Preceding gene: 209398957

Following gene: 209397217

Centisome position: 29.1

GC content: 52.57

Gene sequence:

>915_bases
ATGTCTCTGTTTCACTTAATTGCCCCATCGGGTTACTGCATTAAACAGCACGCCGCGCTGCGTGGTATCCAGCGCCTTAC
CGACGCGGGGCATCAAGTCAATAACGTCGAGGTTATTGCCCGTCGTTGTGAGCGTTTTGCGGGTACGGAAACGGAGCGTC
TGGAAGATCTCAATTCCCTTGCCAGACTGACTACCCCCAACACCATCGTGCTGGCTGTACGCGGCGGTTACGGTGCCAGT
CGTTTACTGGCAGATATTGACTGGCAGGCACTGGTGGCTCGCCAACAATATGACCCGTTGCTCATATGCGGGCATAGCGA
TTTTACCGCCATTCAGTGCGGTCTTCTGGCGCAAGGCAATGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTG
GCGCGGATGAACTGAACACCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTTACCATTGAATGGCAA
GGGGAAGGCCCGACATGTCGGGCCGAAGGCACGTTGTGGGGAGGCAATCTTGCGATGCTGATTTCACTGATCGGTACACC
GTGGATGCCAAAAATTGAGAACGGTATTCTGGTGCTGGAAGATATTAACGAGCACCCTTTCCGCGTCGAACGTATGCTGT
TGCAGCTCTATCATGCGGGAATTTTGCCACGTCAGAAGGCGATTATTCTCGGTAGCTTTAGCGGCAGTACGCCTAATGAT
TATGACGCAGGTTACAACCTGGAGTCAGTGTACGCATTTTTGCGTTCCCGCCTGTCGATTCCACTCATTACCGGCCTCGA
TTTTGGTCATGAACAACGCACGGTTACGCTGCCGTTGGGCGCACATGCCATTCTTACTAACACCCGGGAAGGCACTCAGT
TGACGATTAGCGGTCATCCTGTTCTTAAAATGTAA

Upstream 100 bases:

>100_bases
CTTCTTCTATCCTTATAGCCTGGAATCGCCGTTGAGGTGACAACGGCACACCTAAGCGGCATGATATACATTTCGTATCT
GTTGTCAGCAAGGAATTGCC

Downstream 100 bases:

>100_bases
AAAAAATCACGCTTCAGGGCTAAGTAAAACGCTGTCTCTGCCGCTAATATGGTAGGGTTTATATAAGAGACAGCGTAATC
AGGAGTAACCGACCGTTGGA

Product: L,D-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase A; Muramoyltetrapeptide carboxypeptidase

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGAS
RLLADIDWQALVARQQYDPLLICGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILTNTREGTQLTISGHPVLKM

Sequences:

>Translated_304_residues
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGAS
RLLADIDWQALVARQQYDPLLICGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILTNTREGTQLTISGHPVLKM
>Mature_303_residues
SLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASR
LLADIDWQALVARQQYDPLLICGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQG
EGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPNDY
DAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILTNTREGTQLTISGHPVLKM

Specific function: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. Can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc- tetrapeptide, G

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family

Homologues:

Organism=Escherichia coli, GI1787441, Length=304, Percent_Identity=98.6842105263158, Blast_Score=617, Evalue=1e-178,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LDCA_ECO57 (Q8XDJ8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G85697
- PIR:   G90839
- RefSeq:   NP_287431.1
- RefSeq:   NP_309714.1
- ProteinModelPortal:   Q8XDJ8
- SMR:   Q8XDJ8
- EnsemblBacteria:   EBESCT00000028937
- EnsemblBacteria:   EBESCT00000057294
- GeneID:   913181
- GeneID:   960229
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1955
- KEGG:   ecs:ECs1688
- GeneTree:   EBGT00050000010267
- HOGENOM:   HBG497193
- OMA:   NLAMLCS
- ProtClustDB:   PRK11253
- BioCyc:   ECOL83334:ECS1688-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003507

Pfam domain/function: PF02016 Peptidase_S66

EC number: NA

Molecular weight: Translated: 33602; Mature: 33471

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: ACT_SITE 106-106 ACT_SITE 200-200 ACT_SITE 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSL
CCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLICGHSDFTAIQCGLLAQGN
HHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCC
VITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQGEGPTCRAEGTLWGGNLAML
EEEEECCEEEEECCCHHHHHHHCCEEEEEEECCEEEEEECCCCCEEEECCEEECCCHHHH
ISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILTNTREGTQLTISGHP
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCC
VLKM
EECC
>Mature Secondary Structure 
SLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSL
CEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLICGHSDFTAIQCGLLAQGN
HHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCC
VITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQGEGPTCRAEGTLWGGNLAML
EEEEECCEEEEECCCHHHHHHHCCEEEEEEECCEEEEEECCCCCEEEECCEEECCCHHHH
ISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILTNTREGTQLTISGHP
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCC
VLKM
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796