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The map label for this gene is intE [H]

Identifier: 209397356

GI number: 209397356

Start: 1737316

End: 1738446

Strand: Reverse

Name: intE [H]

Synonym: ECH74115_1821

Alternate gene names: 209397356

Gene position: 1738446-1737316 (Counterclockwise)

Preceding gene: 209400511

Following gene: 209395721

Centisome position: 31.2

GC content: 46.15

Gene sequence:

>1131_bases
ATGGCGCGCCCGCGAAAATATAAAACCGATGTTCCGGGATTATCTCCGTATTTTGACAAAAGAAATAACAAAGTTTACTG
GCGTTACAGGCATCCCATAACAGGCAAAAATCACGGTCTCGGCAGTATTGACCAGAAACTGGCAGAAACTATTGCAGCAG
AAGCGAACAGCCGTCTTGCCCGGCAGCAAATGGAACAAATGCTCAGTCTGCAGGAGAAAATTATTAGTGATACCGGCGGT
TCATCAACCGTTACCATTTTTCTGAATAATTACAGAAAAATTCAACAGGAAAGATATGAAAACGGCGAGATCAAACTCAA
CACGCTGAAACAGAAAGCGGCCCCTCTCAGGGTATTTGATGAACGTTTTGGCACCAGACCGTTAGATGCCATAACCGTAA
AAGATGTGGTATCAGTACTGGAAGAGTACAAGGCCAGAGGACATAACAGAATGGGACAAATTTTCAGGAAAGTACTGATC
GATGTTTTCCGGGAAGCTCAGCAAACGGGCGATGTCCCGCCAGGCTTTAACCCTGCAGAATCTGCAAAAAAACCGCAGGT
GCGGATATCAAGACAGCGACTGACTTTTGATGAGTGGATGATGATTTATAACGCAGCGGAAAAGGATGGTTACTTTTTAC
AGCGCGGTATGCTGCTGGCACTGATGACAGGCCAGCGCCTTTCAGATATTTGCAAAATGCAATTTTCGGATATCCGGGAT
GGTTATCTTCATGTCGAACAGCAAAAAACAGGAACCCGGATTGCCATCCCTCTGGCTCTGCGTTGCGATAAATTAAATCT
CACCCTGGATGATGTGGTGTCATCCTGCCGCGATTGCGTTCTTAGTCCGTGGCTATTGCACCACCATCACGCGAAAGGGA
CAGCTAAGCGCGGCGGGATGGTTAAGCCAGCAACATTAACCGTTGCATTTAAAAAAGCCCGGGATTCTGTGGATTACAAC
TGGCGTGCTAATGGCACCCCACCCTCTTTCCATGAGCAGAGATCTTTATCAGAGCGATTGTTCAGAGAGCAGGGGGTTGA
TACCAAAATTTTGCTAGGCCATTCGAATCAAAAAATGATCGATATTTACAACGACGCACGCGGTAAGGAATGGAAAAAAC
TGGTCATTTGA

Upstream 100 bases:

>100_bases
AGACGCTGGCGAGTGGATCCGCAAGCTCGCTTTGTCGGAATGGTAAACAAGCCGGAGGTGATCGCCACAGATCACCCTGC
TTTGAAGAGGATACTGGAAG

Downstream 100 bases:

>100_bases
TGACCAGTTTTGCAGAGGGGTTTTGCAGAGGTTTTGCAGAGAAAATTAAAAACGATATCCTGCGGAGAACATAAACACCC
ACGGATCCAGTCGTACCGAG

Product: site-specific recombinase, phage integrase family

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSIDQKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGG
SSTVTIFLNNYRKIQQERYENGEIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRD
GYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYN
WRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMIDIYNDARGKEWKKLVI

Sequences:

>Translated_376_residues
MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSIDQKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGG
SSTVTIFLNNYRKIQQERYENGEIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRD
GYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYN
WRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMIDIYNDARGKEWKKLVI
>Mature_375_residues
ARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSIDQKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGS
STVTIFLNNYRKIQQERYENGEIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLID
VFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRDG
YLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNW
RANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMIDIYNDARGKEWKKLVI

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=375, Percent_Identity=49.3333333333333, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 43180; Mature: 43049

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSIDQKLAETIAAEANSRLA
CCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENGEIKLNTLKQKAAPLRVFD
HHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHH
ERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLIDVFREAQQTGDVPPGFNPAE
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRD
HCCCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
GYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGM
CEEEEEECCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCC
VKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMI
CCCCEEEEEEHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
DIYNDARGKEWKKLVI
EEECCCCCCCHHHCCC
>Mature Secondary Structure 
ARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSIDQKLAETIAAEANSRLA
CCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENGEIKLNTLKQKAAPLRVFD
HHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHH
ERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLIDVFREAQQTGDVPPGFNPAE
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRD
HCCCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
GYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGM
CEEEEEECCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCC
VKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMI
CCCCEEEEEEHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
DIYNDARGKEWKKLVI
EEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]