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The map label for this gene is recE [H]

Identifier: 209400511

GI number: 209400511

Start: 1738865

End: 1741180

Strand: Reverse

Name: recE [H]

Synonym: ECH74115_1822

Alternate gene names: 209400511

Gene position: 1741180-1738865 (Counterclockwise)

Preceding gene: 209399784

Following gene: 209397356

Centisome position: 31.25

GC content: 49.4

Gene sequence:

>2316_bases
ATGAGCACTGATAAAGAAGAAATTGCACTGTATTACGAAGCCAAAAATGACAAAGTCAGAAAACGCCTTGGGATTAAAGG
CGGTTTTTACTGGCGCACAGCAAAAAAATTATCGGTTGCAATATCACGGGGTGTTGTCGCAATGGACGATGCTGGATTTG
ACGAAGAGGATTTCAAAAAACCTGTTCGCGTGAATTTGCCCATTGTTAATGACCTGCCGCCTGAAGGTGTGTTCGATACT
GAATTCTGCAACCGCTATGAAAAAGGCGGGGAAGATGGCATCACAATGATATTTATAGCGCCTTCCCCCTCAGTTCAGGA
CAAACCAGCCAGCTCTGACAATACCAACGTCAATGGCGAAGACATGGCTGAGATTGAGGATAATATGCTCCTGCCGATTT
CCGGTCAGGAACTGCCCATTCGCTGGCTTGCGCAACATGGCAGCGAAAAACCGGTAACGCACGTTTCACGGGAAGAACTT
CAGGCATTACATATCGCACGAGCTGAAGAACTGCCTGCTGTTACTGCCCTGGCTATTTCCCACAACACAAAGCTGCTCGA
CCCGCTGGAGATTCGCGACCTTCACAAACTGGTACGCGACACAGACAAAGTTTTCCCTAATCCCGTTAATTCCAGTCTGG
GGTTAATGACTGCTTTTTTCGAAGCATACCTGGACGCTGACTATACCGATCGAGGTCTGCTGACAAAAGAGTGGATGAAA
GGAAATCGTGTTTTACGCATCAGCCGCACGCCATCCGGCGCTAATGCTGGCGGAGGAATTCTTACCGATCGCGGTGAAAG
TTTTGTCCACGATGATGCGTCAGTAGAACGTGACGTTGCCGCTGGCGTTCTGGCCCGTTCAATGGACATCGATATTTACA
ATCCACATCCGGCACACGCCAAACGCATTGAAGAAATCGTTTCAGAGAATAAGCCGCCCTTTTCTGTTTTTCGTGACAAA
TTCATCGCCATGCCTGGTCACCTGGATTATTCCCGCGCGATAGTGGTTGCGTCCGTGAAAGAAGCACCAATTGGTATCGA
GGCTACTCCCCACCGTGTTACCGAATATCTGAACAAAGTACTGACCGAAACCGACCATGCCAACCCTGATCCAGAAATCG
TGGATATTGCCTGCGGTCGCTCCTCTGCTCCAATGCCGCAGCGTGTAACAAAAGAAGGAAAACAGGATGATGAAGAAAAA
CCGCAGCCATCTGGCGCAATGGCAGATGAACAGGCAACGACTGAAGCAGTGGAACCGGATACAACTGAACATAATCAGGA
CACGCAGTCGATGGATGCTCAGCCACAGATAAATTCTGTTGATGCGAAATATCAGAAACTGCGGGCAGAACTCCATGAAG
CCCGGAAAAACATTCCGCCCCAAAATCCTGTCGATGCAGACAAATTACTGGCTGCCTCTCGCGGAGAATTTGTTGAAGGG
ATTAGCGACCCGAATGATCCGAAATGGATTAAGGGGATCCAGACCCGCGATTCTGTGTACCAGAATCAGCCAGAAACGGA
ACAGAACGACCAGAAAGCGGAACAGAACAGCCCAAATACGCAACAAAACGAGCCAGAAACGAAACAACCTGAACCAGTAG
TGCAACAGGAACCGGAAAAGATCTGCACCGCCTGCGGTCAGAGGAGTGGCGGCAACTGCCCTGATTGTGGCGCGGTGATG
GGCGACGCAACATACCAGGAAACATTCGATGACAAGAACCTGGTTGAAGTTCAGGAAGACGATTCGGAGAAAATGGAAGG
CGCTGAACATCCACACAAGGAGAATGCTGGCAGCGCTCAGGACCACGCCAGCGATAGTGAAACTGGCGAGACGGCAGATC
CCTTAATTACGGTGAACGGTCATCGCATTATCACATCCACCAGCAGGACGTGTGACCATCTAATGATCGACCTTGAAACC
ATGGGAAAAAATCCTGATGCCCCGATTATCTCAATAGGTGCAATATTTTTCGATCCGCAAACCGGAGATATGGGACCGGA
ATTTAGTAAGACTATCGATCTGGAAACTGCTGGCGGAGTCATTGATCGGGACACCATTAAATGGTGGCTTAAGCAATCAC
GCGAAGCGCAATCTGCCATTATGACCGATGAAATCCCGTTAGATGATGCACTGTTACAATTGCGGGAATTTATCGACGAA
AACTCCGGTGAATTTTTTGTTCAGGTCTGGGGAAATGGAGCCAACTTCGACAACACGATTTTGCGCCGTTCATACGAACG
GCAGGGATCCCCTGCCCGTGGCGTTACTACAACGATCGCGATGTACGCACAATCGTTGAGCTGGGGAAAGCCATAG

Upstream 100 bases:

>100_bases
CTGGATAACCCGCCAGCCGTATTAACGCCATACCCGTTGATTAAGCATGCCAGCAATGGCAGGGATTCGTACAACCTTAA
AATAGTTATGAGGTTTATCA

Downstream 100 bases:

>100_bases
ACTTCGATGCCAGAACGGCTATTCCATTCGAAGGTGAGCGCCACAATGCGCTGGATGACGCTCGTTACCAGGCAAAATAC
GTTTCAGCTATCTGGCAAAA

Product: exonuclease family protein

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 771; Mature: 770

Protein sequence:

>771_residues
MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRGVVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDT
EFCNRYEKGGEDGITMIFIAPSPSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTAFFEAYLDADYTDRGLLTKEWMK
GNRVLRISRTPSGANAGGGILTDRGESFVHDDASVERDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDK
FIAMPGHLDYSRAIVVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKEGKQDDEEK
PQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEG
ISDPNDPKWIKGIQTRDSVYQNQPETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVM
GDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRIITSTSRTCDHLMIDLET
MGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDE
NSGEFFVQVWGNGANFDNTILRRSYERQGSPARGVTTTIAMYAQSLSWGKP

Sequences:

>Translated_771_residues
MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRGVVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDT
EFCNRYEKGGEDGITMIFIAPSPSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTAFFEAYLDADYTDRGLLTKEWMK
GNRVLRISRTPSGANAGGGILTDRGESFVHDDASVERDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDK
FIAMPGHLDYSRAIVVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKEGKQDDEEK
PQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEG
ISDPNDPKWIKGIQTRDSVYQNQPETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVM
GDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRIITSTSRTCDHLMIDLET
MGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDE
NSGEFFVQVWGNGANFDNTILRRSYERQGSPARGVTTTIAMYAQSLSWGKP
>Mature_770_residues
STDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRGVVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTE
FCNRYEKGGEDGITMIFIAPSPSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREELQ
ALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTAFFEAYLDADYTDRGLLTKEWMKG
NRVLRISRTPSGANAGGGILTDRGESFVHDDASVERDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKF
IAMPGHLDYSRAIVVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKEGKQDDEEKP
QPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEGI
SDPNDPKWIKGIQTRDSVYQNQPETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMG
DATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRIITSTSRTCDHLMIDLETM
GKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDEN
SGEFFVQVWGNGANFDNTILRRSYERQGSPARGVTTTIAMYAQSLSWGKP

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=560, Percent_Identity=74.6428571428571, Blast_Score=845, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 85270; Mature: 85139

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRGVVAMDDAGFDEEDFKK
CCCCCCEEEEEEECCCHHHHHHHCCCCCEEEEHHHHHHHHHHCCEEEECCCCCCHHHCCC
PVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPSPSVQDKPASSDNTNVNGE
CEEEECCEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
DMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREELQALHIARAEELPAVTALAIS
HHHHHCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
HNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTAFFEAYLDADYTDRGLLTKEWMK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GNRVLRISRTPSGANAGGGILTDRGESFVHDDASVERDVAAGVLARSMDIDIYNPHPAHA
CCEEEEEECCCCCCCCCCCCEECCCCCHHCCCCHHHHHHHHHHHHHHCEEEEECCCCHHH
KRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAIVVASVKEAPIGIEATPHRVTEYLNKV
HHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LTETDHANPDPEIVDIACGRSSAPMPQRVTKEGKQDDEEKPQPSGAMADEQATTEAVEPD
HHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
TTEHNQDTQSMDAQPQINSVDAKYQKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEG
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHC
ISDPNDPKWIKGIQTRDSVYQNQPETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEK
CCCCCCCHHHHCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHH
ICTACGQRSGGNCPDCGAVMGDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQ
HHHHHCCCCCCCCCCHHHHHCCCHHHHHCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCH
DHASDSETGETADPLITVNGHRIITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQ
HCCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHEEEHHHCCCCCCCCEEEEEEEEECCC
TGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDE
CCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
NSGEFFVQVWGNGANFDNTILRRSYERQGSPARGVTTTIAMYAQSLSWGKP
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRGVVAMDDAGFDEEDFKK
CCCCCEEEEEEECCCHHHHHHHCCCCCEEEEHHHHHHHHHHCCEEEECCCCCCHHHCCC
PVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPSPSVQDKPASSDNTNVNGE
CEEEECCEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
DMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREELQALHIARAEELPAVTALAIS
HHHHHCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
HNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTAFFEAYLDADYTDRGLLTKEWMK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GNRVLRISRTPSGANAGGGILTDRGESFVHDDASVERDVAAGVLARSMDIDIYNPHPAHA
CCEEEEEECCCCCCCCCCCCEECCCCCHHCCCCHHHHHHHHHHHHHHCEEEEECCCCHHH
KRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAIVVASVKEAPIGIEATPHRVTEYLNKV
HHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LTETDHANPDPEIVDIACGRSSAPMPQRVTKEGKQDDEEKPQPSGAMADEQATTEAVEPD
HHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
TTEHNQDTQSMDAQPQINSVDAKYQKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEG
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHC
ISDPNDPKWIKGIQTRDSVYQNQPETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEK
CCCCCCCHHHHCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHH
ICTACGQRSGGNCPDCGAVMGDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQ
HHHHHCCCCCCCCCCHHHHHCCCHHHHHCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCH
DHASDSETGETADPLITVNGHRIITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQ
HCCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHEEEHHHCCCCCCCCEEEEEEEEECCC
TGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDE
CCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
NSGEFFVQVWGNGANFDNTILRRSYERQGSPARGVTTTIAMYAQSLSWGKP
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]