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The map label for this gene is ecpD [H]

Identifier: 209396894

GI number: 209396894

Start: 159864

End: 160589

Strand: Reverse

Name: ecpD [H]

Synonym: ECH74115_0149

Alternate gene names: 209396894

Gene position: 160589-159864 (Counterclockwise)

Preceding gene: 209397435

Following gene: 209396258

Centisome position: 2.88

GC content: 48.35

Gene sequence:

>726_bases
ATGTTTCCCTTGGTTAAAAAAACGGTTAGTGCATTATTTGTGAGTACTCTGTTAGCCAGTGCACCAGCATTCGCTGACAT
TATTATTTCCGGAACACGTATCATTTATAACGCTGATAAAAAAGATGTTAACGTTCGTCTGGAAAATAAAGGGAATCGCC
CCTTACTGATTCAGAACTGGTTGGATACGGGCGATGACAATGCCGATCCCAGCCAGATCAAAGTGCCGTTTACCGCGACC
CCACCAGTTTCACGCGTGGAGCCAAAACGCGGTCAAACCGTAAAAGTGATGTATACCGGTGCAACAGCCCTGCCGAAAGA
CCGTGAAAGCGTCTACTGGTTTAACGTGCTGGAAGTGCCGCCTAAGCCGAAAGATGCTGAGGCTGACAAAAACTTGTTAC
AGCTGGCTTTCCGCACACGCATCAAACTCTTTTATCGCCCGAGTGGGTTACAGGGAGAACCGGCTGAAGCACCGGCGAAA
ATCACCTGGAAATTAAACAACGCTCAGTTACAGGCAAATAACCCAACGCCATATTACGTGTCCTTTAATGAGGTCAAGCT
GGAAAGCGGCGGCAAAACCTACAACGTCAATTCCAGCATGGTCACGCCGTTCAGCCAGGCAAGCTTTGGCGTAACCAGTC
TGCCAGGAAGCGTGTCATCCGGAAAAGTGGTCTTTAAAGCGATCAACGACTTTGGCGGCAACATTGATGGTTCCGCAACA
TTCTAA

Upstream 100 bases:

>100_bases
TTGCTTATAAGTAATTGATGGTGTGTTGAGCATAAGGTTTGCCAGCAGGCAAACCTTATTTTCGCGAATTTAATCTTTCT
ACAGACAGCAAGGAGTGCTT

Downstream 100 bases:

>100_bases
AAAATAAACGGATTCTTTTTAACATCGCCAGGAATGCAACCTTGTACCAGTTTACTCATCAAAAAAGCCGTATCCCGAAA
AAAACGCTACTTGCGGCCTG

Product: putative chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVNVRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTAT
PPVSRVEPKRGQTVKVMYTGATALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSVSSGKVVFKAINDFGGNIDGSAT
F

Sequences:

>Translated_241_residues
MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVNVRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTAT
PPVSRVEPKRGQTVKVMYTGATALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSVSSGKVVFKAINDFGGNIDGSAT
F
>Mature_241_residues
MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVNVRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTAT
PPVSRVEPKRGQTVKVMYTGATALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSVSSGKVVFKAINDFGGNIDGSAT
F

Specific function: Probably mediates assembly of a pilin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=245, Percent_Identity=60.8163265306122, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1788428, Length=209, Percent_Identity=37.799043062201, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786743, Length=233, Percent_Identity=34.7639484978541, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787171, Length=231, Percent_Identity=32.034632034632, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786936, Length=207, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790771, Length=223, Percent_Identity=30.9417040358744, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87081806, Length=222, Percent_Identity=31.0810810810811, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788677, Length=209, Percent_Identity=34.4497607655502, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082203, Length=241, Percent_Identity=30.7053941908714, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26317; Mature: 26317

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVNVRLENKGNRPLLIQNW
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCEEEEEEECCCCCCEEEEEC
LDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGATALPKDRESVYWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECC
PKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAKITWKLNNAQLQANNPTPYYV
CCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECEEEEECCCCEEEE
SFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSVSSGKVVFKAINDFGGNIDGSAT
EEEEEEECCCCEEEECCCCEECCCCCCCCCEECCCCCCCCCEEEEEEEHHCCCCCCCCCC
F
C
>Mature Secondary Structure
MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVNVRLENKGNRPLLIQNW
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCEEEEEEECCCCCCEEEEEC
LDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGATALPKDRESVYWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECC
PKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAKITWKLNNAQLQANNPTPYYV
CCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECEEEEECCCCEEEE
SFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSVSSGKVVFKAINDFGGNIDGSAT
EEEEEEECCCCEEEECCCCEECCCCCCCCCEECCCCCCCCCEEEEEEEHHCCCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]