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The map label for this gene is mtlA [H]

Identifier: 209396799

GI number: 209396799

Start: 4616332

End: 4618245

Strand: Direct

Name: mtlA [H]

Synonym: ECH74115_4969

Alternate gene names: 209396799

Gene position: 4616332-4618245 (Clockwise)

Preceding gene: 209400677

Following gene: 209395785

Centisome position: 82.85

GC content: 53.5

Gene sequence:

>1914_bases
ATGTCATCCGATATTAAGATCAAAGTGCAAAGCTTTGGTCGTTTCCTCAGCAACATGGTGATGCCAAATATCGGCGCGTT
TATCGCGTGGGGTATCATCACCGCGTTATTTATTCCAACAGGGTGGTTACCGAACGAGACGCTGGCGAAGCTGGTCGGGC
CGATGATCACTTATCTCCTGCCGCTGCTGATCGGTTATACCGGTGGTAAGCTGGTAGGCGGCGAACGTGGCGGCGTAGTC
GGTGCCATCACCACCATGGGCGTTATCGTCGGCGCAGACATGCCGATGTTCCTCGGTTCTATGATTGCAGGTCCGCTGGG
CGGCTGGTGCATTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCT
CCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAACCCTGTCCAAAATG
CTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCT
GTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTGGGTATTCAGCAGTCCCATGAACTGGGTAAATCAATCT
TCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAA
CAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGTGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCC
GCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTC
CGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTATTTCGCTAACATCGCGGGTGTGTGT
GCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGC
AGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGTGCATCTCCGCTGTCTGCGGGCGATGTGACTAACGACC
TGAGCCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTGCTGCGTAAG
AAAATTCAGGATGCAGGTCTGTCACAGATTTCGGTCACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGT
CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCTCAGGCACAGCATATTTCGCTAACCAACTTCCTCG
ACAGCGGCCTGTACACCAGCCTGACTGAACGTCTGGTTGCTGCCCAGCGACATACTGAAAACGAAGTGAAAGTGAAAGAC
AGTCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAACTGGGCGCGGAGAACATCTTCCTCGGTCGCAAAGC
AGCAACCAAAGAAGAAGCGATTCGTTTTGCTGGCGAGCAGCTGGTGAAAGGCGGTTACGTTGAGCCGGAGTACGTTCAGG
CGATGCTGGATCGTGAAAAACTGACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAA
GATCGCGTACTGAAAACGGGCGTGGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGC
TCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAACGCACTGGATGATG
AGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAA

Upstream 100 bases:

>100_bases
TTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCACATTAACAAAAAACCTCGGGCTTCCAGCCTGCGCGACAGCAA
ACATAAGAAGGGGTGTTTTT

Downstream 100 bases:

>100_bases
TCCAATCCTACCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGAAAAGCCTCACCCCAGCCCTCTCGGGTAAAAACATTGA
TGAAGGTTAATACTATGAAA

Product: PTS system, mannitol-specific IIABC component

Products: NA

Alternate protein names: EIICBA-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component; Mannitol-specific phosphotransferase enzyme IIA component; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVV
GAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVC
AAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRK
KIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTENEVKVKD
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAK
DRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK

Sequences:

>Translated_637_residues
MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVV
GAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVC
AAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRK
KIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTENEVKVKD
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAK
DRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK
>Mature_636_residues
SSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVG
AITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKML
AAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQ
SAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCA
AMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKK
IQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTENEVKVKDS
LKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKD
RVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=637, Percent_Identity=99.5290423861852, Blast_Score=1284, Evalue=0.0,
Organism=Escherichia coli, GI1789301, Length=469, Percent_Identity=52.6652452025586, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1788494, Length=145, Percent_Identity=46.2068965517241, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 68031; Mature: 67900

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLL
CCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
PLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDG
HHHHCCCCCEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
KIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKMLAAGVNFMVVHDMLPLASIF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
HCCHHEEEECCHHHCCCCCCCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCC
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSI
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCE
LAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQISVTNSAINNL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHCC
PPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTENEVKVKD
CCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHH
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREK
HHHHCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEH
CCHHHCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCH
IQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLL
CCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
PLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDG
HHHHCCCCCEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
KIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKMLAAGVNFMVVHDMLPLASIF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
HCCHHEEEECCHHHCCCCCCCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCC
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSI
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCE
LAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQISVTNSAINNL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHCC
PPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTENEVKVKD
CCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHH
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREK
HHHHCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEH
CCHHHCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCH
IQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6309813; 8041620; 9278503; 3135464; 3142516; 1946374; 9551558 [H]