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The map label for this gene is hybE

Identifier: 209396299

GI number: 209396299

Start: 3983747

End: 3984235

Strand: Reverse

Name: hybE

Synonym: ECH74115_4304

Alternate gene names: 209396299

Gene position: 3984235-3983747 (Counterclockwise)

Preceding gene: 209397274

Following gene: 209396775

Centisome position: 71.5

GC content: 54.6

Gene sequence:

>489_bases
ATGACTGAAGAGATAGCAGGTTTCCAGACCTCCCCGAAGGCGCAAGTACAGGCAGCGTTTGAAGAAATTGCCCGGCGTTC
GATGCACGCTCTCTCTTTTCTGCATCCTTCAATGCCGGTGTATGTTTCTGATTTTACGCTGTTCGAAGGTCAGTGGACGG
GGTGTGTGATCACCCCGTGGATGCTGAGTGCAGTTATCTTCCCCGGCCCGGATCAACTCTGGCCGCTGCGCAAAGTGAGT
GAAAAAATTGGTCTGCAACTGCCGTATGGCACCATGACCTTTACCGTTGGCGAACTGGACGGTGTTTCGCAATACCTCTC
CTGTTCGCTGATGTCACCGCTTTCGCACAGTATGTCGATTGAAGAGGGCCAACGCCTGACGGATGACTGCGCACGAATGA
TCCTTTCGCTGCCAGTCACGAATCCGGATGTACCACACGCAGGGCGTCGCGCCCTGCTGTTTGGTCGCAGGAGTGGCGAA
AATGCATGA

Upstream 100 bases:

>100_bases
TTAACGCCGACGGTTGAAGCAATGATTGAACCTGCGCTTGAGCAGGTTCTGGCTGCGCTGCGTGAATCTGGCGTGGAAGC
CATCCCACGGGAGGCGATTC

Downstream 100 bases:

>100_bases
GTTGTCGCTTTGCCAGAGCGCCGTTGAAATTATCCAGCGGCAGGCGGAGCAGCACGATGTTAAGCGCGTCACCGCCGTGT
GGCTGGAAATTGGCGCGCTC

Product: hydrogenase 2-specific chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSDFTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVS
EKIGLQLPYGTMTFTVGELDGVSQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
NA

Sequences:

>Translated_162_residues
MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSDFTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVS
EKIGLQLPYGTMTFTVGELDGVSQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
NA
>Mature_161_residues
TEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSDFTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSE
KIGLQLPYGTMTFTVGELDGVSQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGEN
A

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupJ family

Homologues:

Organism=Escherichia coli, GI1789366, Length=162, Percent_Identity=99.3827160493827, Blast_Score=334, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYBE_ECO57 (Q8XBW0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85958
- PIR:   E91113
- RefSeq:   NP_289570.1
- RefSeq:   NP_311904.1
- EnsemblBacteria:   EBESCT00000025563
- EnsemblBacteria:   EBESCT00000056349
- GeneID:   916298
- GeneID:   958475
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4346
- KEGG:   ecs:ECs3877
- GeneTree:   EBGT00050000011454
- HOGENOM:   HBG296627
- OMA:   LTPWMLS
- ProtClustDB:   PRK10465
- BioCyc:   ECOL83334:ECS3877-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 17920; Mature: 17789

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSDFTLFEGQWTGCVITPW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEECCCCCCEEECHH
MLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGVSQYLSCSLMSPLSHSMSI
HHHHEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCH
EEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGENA
HCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSDFTLFEGQWTGCVITPW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEECCCCCCEEECHH
MLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGVSQYLSCSLMSPLSHSMSI
HHHHEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCH
EEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGENA
HCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796