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The map label for this gene is hypA1

Identifier: 209396775

GI number: 209396775

Start: 3983413

End: 3983754

Strand: Reverse

Name: hypA1

Synonym: ECH74115_4303

Alternate gene names: 209396775

Gene position: 3983754-3983413 (Counterclockwise)

Preceding gene: 209396299

Following gene: 209399055

Centisome position: 71.49

GC content: 56.14

Gene sequence:

>342_bases
ATGCATGAGTTGTCGCTTTGCCAGAGCGCCGTTGAAATTATCCAGCGGCAGGCGGAGCAGCACGATGTTAAGCGCGTCAC
CGCCGTGTGGCTGGAAATTGGCGCGCTCTCCTGCGTTGAGGAGAGCGCCGTCCGTTTTAGTTTTGAAATTGTCTGCCACG
GAACGGTGGCGCAAGGGTGCGATTTACATATCGTCTATAAACCCGCCCAGGCCTGGTGCTGGGATTGCAGCCAGGTGGTG
GAGGTTCATCAGCACGATGCGCAGTGTCCGCTCTGTCACGGCGAGCGGTTGCGTGTCGATACCGGCGATTCGCTGATCGT
CAAAAGTATTGAAGTTGAATAA

Upstream 100 bases:

>100_bases
GATGACTGCGCACGAATGATCCTTTCGCTGCCAGTCACGAATCCGGATGTACCACACGCAGGGCGTCGCGCCCTGCTGTT
TGGTCGCAGGAGTGGCGAAA

Downstream 100 bases:

>100_bases
CCGGAGTTAACAATGTGTATTGGCGTTCCAGGCCAGGTGCTGGCTGTCGGTGAAGATATTCACCAGCTTGCGCAGGTTGA
AGTATGTGGTATCAAGCGCG

Product: hydrogenase nickel incorporation protein HybF

Products: NA

Alternate protein names: Hydrogenase-2 operon protein hybF

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV
EVHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE

Sequences:

>Translated_113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV
EVHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
>Mature_113_residues
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV
EVHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family

Homologues:

Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=99.1150442477876, Blast_Score=227, Evalue=2e-61,
Organism=Escherichia coli, GI1789081, Length=114, Percent_Identity=48.2456140350877, Blast_Score=117, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYBF_ECO57 (Q8XBW1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85958
- PIR:   D91113
- RefSeq:   NP_289569.1
- RefSeq:   NP_311903.1
- ProteinModelPortal:   Q8XBW1
- SMR:   Q8XBW1
- EnsemblBacteria:   EBESCT00000028625
- EnsemblBacteria:   EBESCT00000060160
- GeneID:   916300
- GeneID:   958485
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4345
- KEGG:   ecs:ECs3876
- GeneTree:   EBGT00050000009287
- HOGENOM:   HBG688671
- OMA:   MHEMSLC
- ProtClustDB:   PRK12380
- BioCyc:   ECOL83334:ECS3876-MONOMER
- HAMAP:   MF_00213
- InterPro:   IPR000688
- InterPro:   IPR020538
- TIGRFAMs:   TIGR00100

Pfam domain/function: PF01155 HypA

EC number: NA

Molecular weight: Translated: 12684; Mature: 12684

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
DLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
CEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
DLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTGDSLIVKSIEVE
CEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796