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The map label for this gene is speE [H]

Identifier: 209395821

GI number: 209395821

Start: 139951

End: 140817

Strand: Reverse

Name: speE [H]

Synonym: ECH74115_0128

Alternate gene names: 209395821

Gene position: 140817-139951 (Counterclockwise)

Preceding gene: 209397732

Following gene: 209397177

Centisome position: 2.53

GC content: 51.21

Gene sequence:

>867_bases
ATGGCCGAAAAAAAACAGTGGCATGAAACGCTACACGACCAGTTTGGGCAGTACTTTGCGGTAGATAACGTTCTGTATCA
TGAAAAGACCGATCACCAGGATCTGATCATTTTTGAGAACGCTGCATTTGGTCGCGTAATGGCGCTGGATGGCGTAGTAC
AAACCACCGAGCGCGACGAGTTTATCTATCATGAGATGATGACCCATGTTCCGCTACTGGCCCACGGTCACGCGAAACAT
GTGCTGATTATCGGCGGCGGCGACGGTGCCATGCTGCGTGAAGTAACCCGACATAAAAACGTTGAGTCAATCACGATGGT
GGAAATCGATGCGGGTGTCGTGTCGTTCTGCCGTCAGTATCTACCCAACCATAACGCCGGTAGCTACGACGATCCGCGCT
TTAAGCTGGTGATCGACGATGGCGTCAATTTCGTTAATCAAACCAGCCAGACCTTTGATGTCATTATCTCCGACTGCACC
GATCCTATCGGTCCCGGCGAAAGCCTTTTCACTTCGGCATTTTATGAAGGCTGCAAACGTTGCCTGAATCCTGGCGGTAT
CTTCGTCGCACAAAACGGCGTCTGCTTTTTACAGCAGGAAGAAGCCATCGACAGCCATCGCAAACTCAGCCATTACTTCA
GCGACGTTGGCTTTTATCAGGCGGCGATCCCGACCTATTACGGCGGTATCATGACTTTTGCATGGGCGACAGATAACGAC
GCCTTACGCCATCTCTCAACCGAAATTATTCAGGCGCGTTTTCTCGCCTCTGGCCTGAAATGCCGTTATTACAATCCGGC
AGTCCATACGGCAGCTTTTGCCTTACCCCAGTATCTGCAAGACGCACTGGCTTCACAGCCGTCCTAA

Upstream 100 bases:

>100_bases
GATACCTTTTTGTAGAAATATTGACCGTGCATCCGCGGTCAATGTTAGCTATTATGTTGCGCCCTTTTTTTACGGGTGTT
AACAAAGGAGGTATCAACCC

Downstream 100 bases:

>100_bases
GGAGAAGATAAGAAATTGAAAAAACTGAAACTGCATGGCTTTAATAATCTGACCAAAAGTCTGAGTTTTTGTATTTACGA
TATCTGCTACGCCAAAACTG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKH
VLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
ALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALPQYLQDALASQPS

Sequences:

>Translated_288_residues
MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKH
VLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
ALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALPQYLQDALASQPS
>Mature_287_residues
AEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHV
LIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCTD
PIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDNDA
LRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALPQYLQDALASQPS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=286, Percent_Identity=36.7132867132867, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1786312, Length=288, Percent_Identity=99.6527777777778, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25154106, Length=279, Percent_Identity=40.8602150537634, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI212645616, Length=223, Percent_Identity=42.6008968609865, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=38.4615384615385, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6325326, Length=271, Percent_Identity=36.5313653136531, Blast_Score=188, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24645443, Length=267, Percent_Identity=38.2022471910112, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=36.2790697674419, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32308; Mature: 32176

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDE
CCCHHHHHHHHHHHCCCEEECCCEEECCCCCCCCEEEEECCCCCEEEECCCHHHCCCCHH
FIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQY
HHHHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCTDPIGPGESLFTSAFYEGCKR
CCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
HCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALPQYLQDALASQPS
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDE
CCHHHHHHHHHHHCCCEEECCCEEECCCCCCCCEEEEECCCCCEEEECCCHHHCCCCHH
FIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQY
HHHHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCTDPIGPGESLFTSAFYEGCKR
CCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
HCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALPQYLQDALASQPS
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA