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The map label for this gene is wcaA [H]

Identifier: 209395746

GI number: 209395746

Start: 2772768

End: 2773607

Strand: Reverse

Name: wcaA [H]

Synonym: ECH74115_2994

Alternate gene names: 209395746

Gene position: 2773607-2772768 (Counterclockwise)

Preceding gene: 209396474

Following gene: 209395739

Centisome position: 49.78

GC content: 51.07

Gene sequence:

>840_bases
ATGAAAGACAATCCGCTGATCTCAATCTATATGCCGACCTGGAACCGCCAACAACTGGCGATTCGGGCGATAAAATCGGT
CTTGCGCCAGGACTACAGCAACTGGGAGATGATCATCGTGGATGATTGCTCCACCTCCTGGGAACAACTGCAACAGTACG
TCACCGCCCTCAACGATCCGCGTATTACCTACATTCACAACGACATTAACAGCGGGGCGTGCACGGTACGTAACCAGGCG
ATTATGCTGGCGCAAGGGGAATACATTACCGGGATCGATGACGATGATGAATGGACGCCCAACCGCCTGAGCGTCTTCCT
CGCCCATAAACAGCAACTGGTCACTCACGCCTTTTTGTACGCTAACGATTACGTTTGCCAGGGCGAAGTTTATTCCCAAC
CGGCAAGCTTACCACTGTATCCCAAATCGCCGTACTCACGCCGCCTGTTCTATAAACGCAATATCATTGGCAATCAGGTC
TTTACCTGGGCATGGCGTTTCAAAGAGTGTTTGTTCGATACCGAACTGAAAGCGGCACAGGATTACGACATCTTCCTGCG
GATGGTGGTGGAGTATGGCGAACCGTGGAAAGTGGAAGAGGCGACGCAGATCCTGCATATCAATCACGGTGAAATGCAGA
TCACCTCGTCACCGAAAAAATTCTCCGGATACTTCCACTTTTACCGCAAGCACAAAGACAAATTCGACCGTGCCAGTAAA
AAATATCAGCTGTTTACCCTCTACCAGATCCGCAATAAACGTATGACCTGGCGCACTTTGCTGACGCTGCTGTCGGTGCG
TAACGGCAAACGTCTGGCTGATGGTATTCGGGGGCGCTAA

Upstream 100 bases:

>100_bases
CGAAATAACGAGGCCTGCATTCGCACCGCCCCGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCAAGCAAACCAGCTC
ATAAGCCGGGAGTACAACCT

Downstream 100 bases:

>100_bases
ATATGCTGGAAGATCTACGCGCCAACAGCTGGAGTTTACGCCCGTGCTGCATGGTTCTTGCCTATCGCGTCGCTCATTTT
TGTTCGGTGTGGCGCAAAAA

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR

Sequences:

>Translated_279_residues
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR
>Mature_279_residues
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti exoO [H]

Homologues:

Organism=Escherichia coli, GI1788372, Length=279, Percent_Identity=99.2831541218638, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 33066; Mature: 33066

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
RITYIHNDINSGACTVRNQAIMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLY
EEEEEECCCCCCEEEEECCEEEEECCCEEECCCCCCCCCCCHHEEEHHHHHHHHHHHHHH
ANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKECLFDTELKAAQ
CCCEEECCHHHCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHCCHHCCCC
DYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
CHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR
CEEEEEEEEHHCCCHHHHHHHHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
RITYIHNDINSGACTVRNQAIMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLY
EEEEEECCCCCCEEEEECCEEEEECCCEEECCCCCCCCCCCHHEEEHHHHHHHHHHHHHH
ANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKECLFDTELKAAQ
CCCEEECCHHHCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHCCHHCCCC
DYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
CHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR
CEEEEEEEEHHCCCHHHHHHHHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]