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The map label for this gene is wcaB

Identifier: 209395739

GI number: 209395739

Start: 2772277

End: 2772765

Strand: Reverse

Name: wcaB

Synonym: ECH74115_2993

Alternate gene names: 209395739

Gene position: 2772765-2772277 (Counterclockwise)

Preceding gene: 209395746

Following gene: 209398209

Centisome position: 49.76

GC content: 53.78

Gene sequence:

>489_bases
ATGCTGGAAGATCTACGCGCCAACAGCTGGAGTTTACGCCCGTGCTGCATGGTTCTTGCCTATCGCGTCGCTCATTTTTG
TTCGGTGTGGCGCAAAAAGAACGTTCTCAACAATCTTTGGGCGGCCCCGCTGCTGGTGCTGTATCGCATTATCACCGAAT
GCTTTTTCGGTTATGAAATCCAGGCTGCCGCGACCATTGGCCGCCGCTTTACTATCCATCACGGTTACGCCGTAGTGATC
AATAAAAACGTGGTAGCGGGGGATGATTTCACCATTCGCCACGGCGTCACTATCGGCAATCGTGGTGCCGATAACATGGC
ATGTCCACACATTGGCAACGGCGTCGAACTCGGTGCCAACGTCATTATTCTTGGTGATATCACGCTTGGTAACAACGTCA
CCGTGGGCGCGGGCAGCGTGGTGCTCGACTCTGTCCCGGACAACGCGCTGGTGGTGGGAGAAAAAGCGCGAGTGAAGGTA
ATTAAATGA

Upstream 100 bases:

>100_bases
ACCAGATCCGCAATAAACGTATGACCTGGCGCACTTTGCTGACGCTGCTGTCGGTGCGTAACGGCAAACGTCTGGCTGAT
GGTATTCGGGGGCGCTAAAT

Downstream 100 bases:

>100_bases
ATATTCTGCAATTTAATGTGCGACTGGCGGAAGGCGGGGCGGCAGGTGTGGCGTTAGATCTCCACCAGCGTGCGCTGCAA
CAGGGGCTGGCGTCACATTT

Product: putative colanic acid biosynthesis acetyltransferase WcaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPLLVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVI
NKNVVAGDDFTIRHGVTIGNRGADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
IK

Sequences:

>Translated_162_residues
MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPLLVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVI
NKNVVAGDDFTIRHGVTIGNRGADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
IK
>Mature_162_residues
MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPLLVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVI
NKNVVAGDDFTIRHGVTIGNRGADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
IK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG1045

COG function: function code E; Serine acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1788371, Length=162, Percent_Identity=100, Blast_Score=329, Evalue=6e-92,
Organism=Escherichia coli, GI1790035, Length=140, Percent_Identity=32.8571428571429, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WCAB_ECO57 (P0ACD0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85832
- PIR:   G90986
- RefSeq:   NP_288564.1
- RefSeq:   NP_310890.1
- ProteinModelPortal:   P0ACD0
- SMR:   P0ACD0
- EnsemblBacteria:   EBESCT00000026931
- EnsemblBacteria:   EBESCT00000056890
- GeneID:   912644
- GeneID:   958129
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3222
- KEGG:   ecs:ECs2863
- GeneTree:   EBGT00050000009073
- HOGENOM:   HBG754554
- OMA:   TIGDHVE
- ProtClustDB:   PRK10191
- BioCyc:   ECOL83334:ECS2863-MONOMER
- InterPro:   IPR018357
- InterPro:   IPR005881
- InterPro:   IPR011004
- PIRSF:   PIRSF000441

Pfam domain/function: SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 17616; Mature: 17616

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPLLVLYRIITECFFGYEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
QAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRGADNMACPHIGNGVELGAN
EEEECCCCEEEEECCEEEEEECCEEECCCEEEEECEEECCCCCCCCCCCCCCCCEECCCC
VIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKVIK
EEEEEEEEECCCEEECCCCEEEECCCCCEEEECCCCEEEEEC
>Mature Secondary Structure
MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPLLVLYRIITECFFGYEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
QAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRGADNMACPHIGNGVELGAN
EEEECCCCEEEEECCEEEEEECCEEECCCEEEEECEEECCCCCCCCCCCCCCCCEECCCC
VIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKVIK
EEEEEEEEECCCEEECCCCEEEECCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796