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The map label for this gene is nfo [H]

Identifier: 209695970

GI number: 209695970

Start: 2732391

End: 2733239

Strand: Reverse

Name: nfo [H]

Synonym: VSAL_I2552

Alternate gene names: 209695970

Gene position: 2733239-2732391 (Counterclockwise)

Preceding gene: 209695972

Following gene: 209695969

Centisome position: 82.2

GC content: 40.28

Gene sequence:

>849_bases
ATGAAATATATTGGGGCACATGTGTCTGCGGCCGGTGGTGTTGATCAAGCGCCAAAACGTGCATATCAAATGGGGGCTAC
AGCGTTTGCATTATTTACTAAAAATCAGCGCCAATGGGTAGCTAAGCCGTTAACAAAAAAGATCATCTCTGATTTTAAAG
CAAATTGTAAGCGTTTTGGATTTGAACCTTCTCAAATATTACCGCATGACTCTTATTTAATTAATCTTGGTGCGCCAGAA
GAAGAAAAGCTAGAGAAATCTCGCGCGGCATTTATTGATGAAATGGAGCGTTGTGAACAATTAGGGCTCACTTTGCTCAA
TTTTCATCCAGGCAGCCATTTAAAGAAGATCTCAGAAGATGAGTGTCTTACCCTTATTTCTGAATCCATTAATCTCGCGC
ATCAAGCCGTGCCGAATGTGATTGCTGTGATTGAGAATACGGCTGGCCAAGGAACGAATCTTGGATGGAAATTTGAACAT
TTAGCTCAGATTATCAGTCAAGTTGAAGATAAGTCTCGTGTCGGTATATGCCTTGATACATGCCATACATTCGCCGCTGG
TTATGACTTAAGATCTAAAGAGGCTTGCGAGCATACTTTTTCTGAATTTGAACGTATTGTTGGTTTTCAATACTTGCGTG
GTATGCACATCAATGACGCCAAAATTGAGCTAGGCAGTCGTGTCGATCGCCATCAATCTTTAGGTGAAGGCACGATTGGA
TGGGATTGCTTTAAGTACATTATGCAAGATGATCGTTTTAATGGCATTCCTTTAGTATTAGAGACGATAAACCCTGATAT
TTGGAAGGATGAAATCGCTCAACTTAAAGAACTCAGTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GATATTCTCTGTTTTCCGTCCAAGAAAGCAGAGAAGTACTGACAAATAAAGCATTTACCGTAATAATGGACCGAAGTTAA
TTTTGAAAGGAAAGACAGAG

Downstream 100 bases:

>100_bases
ATGAACACATCTTGGAATGACATTTTAGAAACGGAAAAAGAAAAACCGTATTATCAAGAAATGATGACGTATATAAACGA
AGCACGTTCTCAAGGAAAAA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKYIGAHVSAAGGVDQAPKRAYQMGATAFALFTKNQRQWVAKPLTKKIISDFKANCKRFGFEPSQILPHDSYLINLGAPE
EEKLEKSRAAFIDEMERCEQLGLTLLNFHPGSHLKKISEDECLTLISESINLAHQAVPNVIAVIENTAGQGTNLGWKFEH
LAQIISQVEDKSRVGICLDTCHTFAAGYDLRSKEACEHTFSEFERIVGFQYLRGMHINDAKIELGSRVDRHQSLGEGTIG
WDCFKYIMQDDRFNGIPLVLETINPDIWKDEIAQLKELSEKK

Sequences:

>Translated_282_residues
MKYIGAHVSAAGGVDQAPKRAYQMGATAFALFTKNQRQWVAKPLTKKIISDFKANCKRFGFEPSQILPHDSYLINLGAPE
EEKLEKSRAAFIDEMERCEQLGLTLLNFHPGSHLKKISEDECLTLISESINLAHQAVPNVIAVIENTAGQGTNLGWKFEH
LAQIISQVEDKSRVGICLDTCHTFAAGYDLRSKEACEHTFSEFERIVGFQYLRGMHINDAKIELGSRVDRHQSLGEGTIG
WDCFKYIMQDDRFNGIPLVLETINPDIWKDEIAQLKELSEKK
>Mature_282_residues
MKYIGAHVSAAGGVDQAPKRAYQMGATAFALFTKNQRQWVAKPLTKKIISDFKANCKRFGFEPSQILPHDSYLINLGAPE
EEKLEKSRAAFIDEMERCEQLGLTLLNFHPGSHLKKISEDECLTLISESINLAHQAVPNVIAVIENTAGQGTNLGWKFEH
LAQIISQVEDKSRVGICLDTCHTFAAGYDLRSKEACEHTFSEFERIVGFQYLRGMHINDAKIELGSRVDRHQSLGEGTIG
WDCFKYIMQDDRFNGIPLVLETINPDIWKDEIAQLKELSEKK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=282, Percent_Identity=69.8581560283688, Blast_Score=418, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=47.5095785440613, Blast_Score=280, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6322735, Length=281, Percent_Identity=41.6370106761566, Blast_Score=241, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31771; Mature: 31771

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGAHVSAAGGVDQAPKRAYQMGATAFALFTKNQRQWVAKPLTKKIISDFKANCKRFG
CCCCCCCCCCCCCCCHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
FEPSQILPHDSYLINLGAPEEEKLEKSRAAFIDEMERCEQLGLTLLNFHPGSHLKKISED
CCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCHH
ECLTLISESINLAHQAVPNVIAVIENTAGQGTNLGWKFEHLAQIISQVEDKSRVGICLDT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
CHTFAAGYDLRSKEACEHTFSEFERIVGFQYLRGMHINDAKIELGSRVDRHQSLGEGTIG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHH
WDCFKYIMQDDRFNGIPLVLETINPDIWKDEIAQLKELSEKK
HHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYIGAHVSAAGGVDQAPKRAYQMGATAFALFTKNQRQWVAKPLTKKIISDFKANCKRFG
CCCCCCCCCCCCCCCHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
FEPSQILPHDSYLINLGAPEEEKLEKSRAAFIDEMERCEQLGLTLLNFHPGSHLKKISED
CCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCHH
ECLTLISESINLAHQAVPNVIAVIENTAGQGTNLGWKFEHLAQIISQVEDKSRVGICLDT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
CHTFAAGYDLRSKEACEHTFSEFERIVGFQYLRGMHINDAKIELGSRVDRHQSLGEGTIG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHH
WDCFKYIMQDDRFNGIPLVLETINPDIWKDEIAQLKELSEKK
HHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA