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The map label for this gene is yaaJ [H]

Identifier: 209695966

GI number: 209695966

Start: 2728705

End: 2730138

Strand: Reverse

Name: yaaJ [H]

Synonym: VSAL_I2548

Alternate gene names: 209695966

Gene position: 2730138-2728705 (Counterclockwise)

Preceding gene: 209695968

Following gene: 209695965

Centisome position: 82.11

GC content: 41.07

Gene sequence:

>1434_bases
GTGACAAGTATTATTAACTTACTGAATGACCTGTTATGGGGTTCAGTTCTCGTGTATTTATTAGTCGCCATTGGGATCTA
TTTTACGGTTCGTTTAGGCTTTATTCAATTTCGTCATTTTGGGCATATGTTTTCTGTTTTAAAAAACAGCCGTAAATCTG
ATTCTTCTGGTATTTCATCGTTTCAAGCACTATGCACCAGCTTAGCTGCGCGTGTTGGTACGGGGAATATGGCAGGGGTT
GCCGTCGCTTTAACCGCCGGCGGTCCAGGCGCTATTTTCTGGATGTGGCTTATTGCTATGTTGGGTATGGCAACGTCATT
TGCTGAAAGTACATTAGCACAATTATATAAAACCAAAGATGATGAAGGCAACTATCGTGGTGGCCCTGCTTATTATATGG
AAAAAGGATTGGGCATGCGCTGGATGGGCGTATTGTTTTCTGTCTTTTTGATCATCGCTTTTGGTTTGGTATTTAATGCA
GTACAAGCAAACTCGATAGCCAGTGCAATGCAGAATGCATTCGGGTGGCAACCTCTGTATGTCGGTATTGGTATTGTGAT
TATTTCTGCGATTGTTATTTTCGGTGGTTTTCGAACCATTGCTCGAACGGCTGAAATCATAGTACCTGTGATGGCATTAG
CGTACTTAGCGATTGCTTTATATATCATGGTTACAAACATAGAAAAACTACCGGGAGTGGTTACTCTGATCTTTAAGAGT
GCTTTTGGACTTCAAGAAGCCGCTGCTGGTGGTTTAGGTTACGCAATTGCTCAAGCAATGATTAATGGTATTAAGCGCGG
TCTATTCTCAAATGAAGCAGGTATGGGGTCTGCACCTAATGCAGCAGCCTCTGCAACACCTTATCCGCCGCATCCTGCTT
CACAAGGTTATGTTCAAATGCTTGGTGTGTTTATGGATACGATTGTTATCTGTTCATCTACTGTCGCGATTATCTTAATG
TCGGGTGAATATGTACCGCATGGCGAAGTCACTGGTATTGAGTTAACTCAGTTAGCATTAACAAGCCAAGTGGGGGATTG
GGGCGGAATATTTATTGCGGTTGCTATTTTCTTCTTTGCTTTTACCTCTATCATTGCAAATTATTCGTACGCTGAGACTA
ATTTGATTTTCTTGGAGCACAATCATAAAGCCGGGATCGGTATTTTCCGTATCGTGGTTTTAGGTATGGTTATGTTTGGG
GCGTTATCATCATTACCTACGGTATGGGCTTTAGCTGATGTTTCTATGGGGATGATGGCAATAGTGAATTTAATCGCAAT
TTCATTATTGTCAGGAACCGTTATCAAGCTGGCGAAAGATTACAATAAGCAGCTTAGTGAGGGTAAATTACCGACCTTTG
ATAGTAAAGATTACCCTGAATTACATTCTCAATTAGAAGAAGGTATTTGGGATAATAACAAAGAGAAAAACTAA

Upstream 100 bases:

>100_bases
GAATTCCTACATAGATGAAGACGCTTAAAGTTCGTCATTTTTTAGCCACTAAATTTATCAAGTGTGCTATGTCATAACCA
AACTTAGCTGAGAGGTAAGC

Downstream 100 bases:

>100_bases
TTAATTGCTTTTACCCATCAAATAAATAGATAAAGCCACGCTACCTAGCGTGGCTTTTTTGTATCTGAATCGTTACTATC
TTTATATAAATCAGATAAAG

Product: amino-acid transporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTSIINLLNDLLWGSVLVYLLVAIGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDSSGISSFQALCTSLAARVGTGNMAGV
AVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKDDEGNYRGGPAYYMEKGLGMRWMGVLFSVFLIIAFGLVFNA
VQANSIASAMQNAFGWQPLYVGIGIVIISAIVIFGGFRTIARTAEIIVPVMALAYLAIALYIMVTNIEKLPGVVTLIFKS
AFGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFMDTIVICSSTVAIILM
SGEYVPHGEVTGIELTQLALTSQVGDWGGIFIAVAIFFFAFTSIIANYSYAETNLIFLEHNHKAGIGIFRIVVLGMVMFG
ALSSLPTVWALADVSMGMMAIVNLIAISLLSGTVIKLAKDYNKQLSEGKLPTFDSKDYPELHSQLEEGIWDNNKEKN

Sequences:

>Translated_477_residues
MTSIINLLNDLLWGSVLVYLLVAIGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDSSGISSFQALCTSLAARVGTGNMAGV
AVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKDDEGNYRGGPAYYMEKGLGMRWMGVLFSVFLIIAFGLVFNA
VQANSIASAMQNAFGWQPLYVGIGIVIISAIVIFGGFRTIARTAEIIVPVMALAYLAIALYIMVTNIEKLPGVVTLIFKS
AFGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFMDTIVICSSTVAIILM
SGEYVPHGEVTGIELTQLALTSQVGDWGGIFIAVAIFFFAFTSIIANYSYAETNLIFLEHNHKAGIGIFRIVVLGMVMFG
ALSSLPTVWALADVSMGMMAIVNLIAISLLSGTVIKLAKDYNKQLSEGKLPTFDSKDYPELHSQLEEGIWDNNKEKN
>Mature_476_residues
TSIINLLNDLLWGSVLVYLLVAIGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDSSGISSFQALCTSLAARVGTGNMAGVA
VALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKDDEGNYRGGPAYYMEKGLGMRWMGVLFSVFLIIAFGLVFNAV
QANSIASAMQNAFGWQPLYVGIGIVIISAIVIFGGFRTIARTAEIIVPVMALAYLAIALYIMVTNIEKLPGVVTLIFKSA
FGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFMDTIVICSSTVAIILMS
GEYVPHGEVTGIELTQLALTSQVGDWGGIFIAVAIFFFAFTSIIANYSYAETNLIFLEHNHKAGIGIFRIVVLGMVMFGA
LSSLPTVWALADVSMGMMAIVNLIAISLLSGTVIKLAKDYNKQLSEGKLPTFDSKDYPELHSQLEEGIWDNNKEKN

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=476, Percent_Identity=50.6302521008403, Blast_Score=464, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51150; Mature: 51018

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSIINLLNDLLWGSVLVYLLVAIGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDSSGISS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FQALCTSLAARVGTGNMAGVAVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKD
HHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEGNYRGGPAYYMEKGLGMRWMGVLFSVFLIIAFGLVFNAVQANSIASAMQNAFGWQPLY
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VGIGIVIISAIVIFGGFRTIARTAEIIVPVMALAYLAIALYIMVTNIEKLPGVVTLIFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQM
HHCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LGVFMDTIVICSSTVAIILMSGEYVPHGEVTGIELTQLALTSQVGDWGGIFIAVAIFFFA
HHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
FTSIIANYSYAETNLIFLEHNHKAGIGIFRIVVLGMVMFGALSSLPTVWALADVSMGMMA
HHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IVNLIAISLLSGTVIKLAKDYNKQLSEGKLPTFDSKDYPELHSQLEEGIWDNNKEKN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503 [H]